Light Filtering in Microscopy

Abstract

In digital photography and photomicrography, colorization and other quality determining parameters can be influenced by post-processing. Thus, in general, the current “importance” of light filtering seems to be somewhat lower than in the past. Nevertheless, light filters are relevant for optimization of image quality up-till now, especially in microscopy—in visual observations as well as in photomicrography. This chapter gives an introduction to the use of colorless (neutral) and color modulating filters. Results achievable are demonstrated by photomicrographs taken with a standard laboratory microscope. The filters presented are capable of optimizing the intermediate image so that they can be regarded as complementary tools promising substantial improvements of visual observations and photomicrographs—regardless of whether post-processing is additionally carried out or not.