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Salivary Biomarkers in Welfare Studies

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Saliva in Health and Disease

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Abstract

Welfare is a concept with an increasing interest in both humans and animals. The human welfare can be understood in three general ways: (1) the individual experiences of happiness; (2) satisfaction of desires; and (3) enjoyment of certain objective goods, such as health, education, personal relationships and recreation. Meanwhile, animal welfare was defined by five major pillars which remark that welfare is achieved when an animal is free of (1) hunger and thirst; (2) discomfort; (3) pain, injury and disease; (4) fear and distress; and is (5) free to express its normal behavior. In this chapter, salivary biomarkers used to evaluate welfare in humans and animals are reviewed being organized in relation with the major system or axis to which these have been associated.

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References

  • Allgrove JE, Gomes E, Hough J, Gleeson M (2008) Effects of exercise intensity on salivary antimicrobial proteins and markers of stress in active men. J Sports Sci 6:653–661

    Article  Google Scholar 

  • Amico JA, Mantella RC, Vollmer RR, Li X (2004) Anxiety and stress responses in female oxytocin deficient mice. J Neuroendocrinol 16:319–324

    Article  CAS  PubMed  Google Scholar 

  • Aschbacher K, O’Donovan A, Wolkowitz OM, Dhabhar FS, Su Y, Epel E (2013) Good stress, bad stress and oxidative stress: insights from anticipatory cortisol reactivity. Psychoneuroendocrinology 38:1698–1708

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ashina M, Bendtsen L, Jensen R, Sakai F, Olesen J (2000) Possible mechanisms of glyceryl-trinitrate-induced immediate headache in patients with chronic tension-type headache. Cephalalgia 20:919–924

    Article  CAS  PubMed  Google Scholar 

  • Aukrust P, Frøland SS, Liabakk NB, Müller F, Nordøy I, Haug C, Espevik T (1994) Release of cytokines, soluble cytokine receptors, and interleukin-1 receptor antagonist after intravenous immunoglobulin administration in vivo. Blood 84:2136–2143

    Article  CAS  PubMed  Google Scholar 

  • Aydin N, Searchfield GD (2019) Changes in tinnitus and physiological biomarkers of stress in response to short-term broadband noise and sounds of nature. Complement Ther Med 46:62–68

    Article  PubMed  Google Scholar 

  • Bae YJ, Reinelt J, Netto J, Uhlig M, Willenberg A, Ceglarek U, Villringer A, Thiery J, Gaebler M, Kratzsch J (2019) Salivary cortisone, as a biomarker for psychosocial stress, is associated with state anxiety and heart rate. Psychoneuroendocrinology 101:35–41

    Article  CAS  PubMed  Google Scholar 

  • Barranco T, Rubio CP, Tvarijonaviciute A, Rubio M, Damia E, Lamy E, Cugat R, Cerón JJ, Tecles F, Escribano D (2019) Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage. Biochemia Med 29(1):010706

    Google Scholar 

  • Bartley EJ, Palit S, Kuhn BL, Kerr KL, Terry EL, DelVentura JL, Rhudy JL (2015) Natural variation in testosterone is associated with hypoalgesia in healthy women. Clin J Pain 31:730–739

    Article  PubMed  Google Scholar 

  • Benton LA, Yates FE (1990) Ultradian adrenocortical and circulatory oscillations in conscious dogs. Am J Phys 258:R578–R590

    Article  CAS  Google Scholar 

  • Blagrove M, Fouquet NC, Baird AL, Pace-Schott EF, Davies AC, Neuschaffer JC, Henley-Einion JA, Weidemann CT, Thome J, McNamara P, Turnbull OH (2012) Association of Salivary-Assessed Oxytocin and Cortisol Levels with Time of Night and Sleep Stage. J Neural Transm 119:1223–1232

    Article  CAS  PubMed  Google Scholar 

  • Bleakman D, Alt A, Nisenbaum ES (2006) Glutamate receptors and pain. Semin Cell Dev Biol 17:592–604

    Article  CAS  PubMed  Google Scholar 

  • Bosch JA, Veerman EC, de Geus EJ, Proctor GB (2011) α-Amylase as a reliable and convenient measure of sympathetic activity: don’t start salivating just yet! Psychoneuroendocrinology 36:449–453

    Article  CAS  PubMed  Google Scholar 

  • Brown SN, Knowles TG, Wilkins LJ, Chadd SA, Warriss PD (2005) The response of pigs to being loaded or unloaded onto commercial animal transporters using three systems. Vet J 170:91–100

    Article  CAS  PubMed  Google Scholar 

  • Bushong DM, Friend TH, Knabe DA (2000) Salivary and plasma cortisol response to adrenocorticotropin administration in pigs. Lab Anim 34:171–181

    Article  CAS  PubMed  Google Scholar 

  • Carlesso LC, Sturgeon JA, Zautra AJ (2016) Exploring the relationship between disease-related pain and cortisol levels in women with osteoarthritis. Osteoarthr Cartil 24:2048–2054

    Article  CAS  Google Scholar 

  • Carpenter GH, Proctor GB, Anderson LC, Zhang XS, Garrett JR (2000) Immunoglobulin A secretion into saliva during dual sympathetic and parasympathetic nerve stimulation of rat submandibular glands. Exp Physiol 85:281–286

    Article  CAS  PubMed  Google Scholar 

  • Carter C, Pournajafi-Nazarloo SH, Kramer KM, Ziegler TE, White-Traut R, Bello D, Schwertz D (2007) Oxytocin: behavioral associations and potential as a salivary biomarker. Ann N Y Acad Sci 1098:312–322

    Article  CAS  PubMed  Google Scholar 

  • Casal N, Manteca X, Escribano D, Cerón JJ, Fàbrega E (2017) Effect of environmental enrichment and herbal compound supplementation on physiological stress indicators (chromogranin A, cortisol and tumour necrosis factor-α) in growing pigs. Animal 11:1228–1236

    Article  CAS  PubMed  Google Scholar 

  • Chennaoui M, Bougard C, Drogou C, Langrume C, Miller C, Gomez-Merino D, Vergnoux F (2016) Stress biomarkers, mood states, and sleep during a major competition: “success” and “failure” athlete’s profile of high-level swimmers. Front Physiol 14:7–94

    Google Scholar 

  • Choi JC, Chung MI, Lee YD (2012) Modulation of pain sensation by stress-related testosterone and cortisol. Anaesthesia 67:1146–1151

    Article  CAS  PubMed  Google Scholar 

  • Cobb ML, Iskandarani K, Chinchilli VM, Dreschel NA (2016) A systematic review and meta-analysis of salivary cortisol measurement in domestic canines. Domest Anim Endocrinol 57:31–42

    Article  CAS  PubMed  Google Scholar 

  • Contreras-Aguilar MD, Escribano D, Martínez-Subiela S, Martínez-Miró S, Rubio M, Tvarijonaviciute A, Tecles F, Cerón JJ (2017a) Influence of the way of reporting alpha-Amylase values in saliva in different naturalistic situations: a pilot study. PLoS One 12(6):e0180100

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Contreras-Aguilar MD, Tecles F, Martínez-Subiela S, Escribano D, Bernal LJ, Cerón JJ (2017b) Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation. BMC Vet Res 13:266

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Contreras-Aguilar MD, Escribano D, Martín-Cuervo M, Tecles F, Cerón JJ (2018a) Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study. BMC Vet Res 14:156

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Contreras-Aguilar MD, Escribano D, Martínez-Subiela S, Martínez-Miró S, Cerón JJ, Tecles F (2018b) Changes in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory study. BMC Vet Res 14:256

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Contreras-Aguilar MD, Escribano D, Martínez-Miró S, López-Arjona M, Rubio CP, Martínez-Subiela S, Cerón JJ, Tecles F (2019a) Application of a score for evaluation of pain, distress and discomfort in pigs with lameness and prolapses: correlation with saliva biomarkers and severity of the disease. Res Vet Sci 6(126):155–163

    Article  CAS  Google Scholar 

  • Contreras-Aguilar MD, Escribano D, Quiles A, López-Arjona M, Cerón JJ, Martínez-Subiela S, Hevia ML, Tecles F (2019b) Evaluation of new biomarkers of stress in saliva of sheep. Animal 13:1278–1286

    Article  CAS  PubMed  Google Scholar 

  • Contreras-Aguilar MD, Martínez-Subiela S, Cerón JJ, Martín-Cuervo M, Tecles F, Escribano D (2019c) Salivary alpha-amylase activity and concentration in horses with acute abdominal disease: association with outcome. Equine Vet J 51:569–574

    CAS  PubMed  Google Scholar 

  • Contreras-Aguilar MD, Monkeviciene I, Ceron JJ, Silinskas I, Vallejo-Mateo PJ, Tecles F, Martinez-Subiela S, Tvarijonaviciute A, Zelvyte R (2019d) Biochemical changes in saliva of cows with inflammation: a pilot study. Res Vet Sci 124:383–386

    Article  CAS  PubMed  Google Scholar 

  • Contreras-Aguilar MD, Vialaret J, de Périère DD, Escribano D, Lehmann S, Tecles F, Cerón JJ, Hirtz C (2019e) Variation of human salivary alpha-amylase proteoforms in three stimulation models. Clin Oral Investig. https://doi.org/10.1007/s00784-019-03021-9

  • Cook NJ, Schaefer AL, Lepage P, Morgan Jones SD (1996) Salivary vs. serum cortisol for the assessment of adrenal activity in swine. Can J Anim Sci 76:329–335

    Article  CAS  Google Scholar 

  • Coulon M, Lévy F, Ravel C, Nowak R, Boissy A (2014) Mild effects of gestational stress and social reactivity on the onset of mother-young interactions and bonding in sheep. Stress 17:460–470

    Article  PubMed  Google Scholar 

  • Cruz-Almeida Y, Aguirre M, Sorenson H, Tighe P, Wallet SM, Riley JL (2017) 3rd Age differences in salivary markers of inflammation in response to experimental pain: does venipuncture matter? J Pain Res 10:2365–2372

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • da Silva Campos MJ, Souza Alves CC, Barbosa Raposo NR, Ferreira AP, Farinazzo Vitral RW (2010) Influence of salivary secretory immunoglobulin A level on the pain experienced by orthodontic patients. Med Sci Monit 16:CR405–CR409

    PubMed  Google Scholar 

  • Deminice R, Sicchieri T, Payão PO, Jordão AA (2010) Blood and salivary oxidative stress biomarkers following an acute session of resistance exercise in humans. Int J Sports Med 31:599–603

    Article  CAS  PubMed  Google Scholar 

  • Den R, Toda M, Nagasawa S (2007) Circadian rhythm of human salivary chromogranin A. Biomed Res 28:57–60

    Article  CAS  PubMed  Google Scholar 

  • Diez-Ruiz A, Tilz GP, Zangerle R, Baier-Bitterlich G, Wachter H, Fuchs D (1995) Soluble receptors for tumour necrosis factor in clinical laboratory diagnosis. Eur J Haematol 54:1–8

    Article  CAS  PubMed  Google Scholar 

  • Dimsdale JE, O’Connor DT, Ziegler M, Mills P (1992) Chromogranin A correlates with norepinephrine release rate. Life Sci 51:519–525

    Article  CAS  PubMed  Google Scholar 

  • Drouet JB, Fauvelle F, Maunoir-Regimbal S, Fidier N, Maury R, Peinnequin A, Denis J, Buguet A, Canini F (2015) Differences in prefrontal cortex GABA/glutamate ratio after acute restraint stress in rats are associated with specific behavioral and neurobiological patterns. Neuroscience 29:155–165

    Article  CAS  Google Scholar 

  • Duncan IJH (2005) Science-based assessment of animal welfare: farm animals. Sci Tech Rev 24:483–492

    Article  CAS  Google Scholar 

  • Eckersall PD (1995) Acute phase proteins as markers of inflammatory lesions. Comp Haematol Int 5:93–97

    Article  CAS  Google Scholar 

  • Edwards KL, Bansiddhi P, Paris S, Galloway M, Brown JL (2019) The development of an immunoassay to measure immunoglobulin A in Asian elephant feces, saliva, urine and serum as a potential biomarker of well-being. Conserv Physiol 7:coy077

    Article  PubMed  PubMed Central  Google Scholar 

  • Engeland CG, Hugo FN, Hilgert JB, Nascimento GG, Junges R, Lim HJ, Marucha PT, Bosch JA (2016) Psychological distress and salivary secretory immunity. Brain Behav Immun 52:11–17

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Soler L, Gutiérrez AM, Martínez-Subiela S, Cerón JJ (2013) Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress. Animal 7(4):640–647

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Campos PH, Gutiérrez AM, Le Floc’h N, Cerón JJ, Merlot E (2014a) Effect of repeated administration of lipopolysaccharide on inflammatory and stress markers in saliva of growing pigs. Vet J 200:393–397

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Fuentes-Rubio M, Cerón JJ (2014b) Salivary testosterone measurements in growing pigs: validation of an automated chemiluminescent immunoassay and its possible use as an acute stress marker. Res Vet Sci 97:20–25

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Gutiérrez AM, Fuentes-Rubio M, Cerón JJ (2014c) Saliva chromogranin A in growing pigs: a study of circadian patterns during daytime and stability under different storage conditions. Vet J 199(3):355–359

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Gutiérrez AM, Tecles F, Cerón JJ (2015) Changes in saliva biomarkers of stress and immunity in domestic pigs exposed to a psychosocial stressor. Res Vet Sci 102:38–44

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Horvatić A, Contreras-Aguilar MD, Guillemin N, Cerón JJ, Tecles F, Martinez-Miró S, Eckersall PD, Manteca X, Mrljak V (2019a) Changes in saliva proteins in two conditions of compromised welfare in pigs: an experimental induced stress by nose snaring and lameness. Res Vet Sci 125:227–234

    Article  CAS  PubMed  Google Scholar 

  • Escribano D, Ko HL, Chong Q, Llonch L, Manteca X, Llonch P (2019b) Salivary biomarkers to monitor stress due to aggression after weaning in piglets. Res Vet Sci 123:178–183

    Article  CAS  PubMed  Google Scholar 

  • Fàbrega E, Marcet-Rius M, Vidal R, Escribano D, Cerón JJ, Manteca X, Velarde A (2019) The effects of environmental enrichment on the physiology, behaviour, productivity and meat quality of pigs raised in a hot climate. Animals (Basel) 9:E235

    Article  Google Scholar 

  • Fischer M, Wille G, Klien S, Shanib H, Holle D, Gaul C, Broessner G (2012) Brain-derived neurotrophic factor in primary headaches. J Headache Pain 13:469–475

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Forsyth IA, Margaret CN (2009) Introduction: the myoepithelial cell and milk letdown; entrance to the multifunctional role of oxytocin. J Mammary Gland Biol Neoplasia 14:221–222

    Article  PubMed  Google Scholar 

  • Fuentes M, Tecles F, Gutiérrez A, Otal J, Martínez-Subiela S, Cerón JJ (2011) Validation of an automated method for salivary alpha-amylase measurements in pigs (Sus scrofa domesticus) and its application as a stress biomarker. J Vet Diagn Investig 23:282–287

    Article  Google Scholar 

  • Fuentes-Rubio M, Fuentes F, Otal J, Quiles A, Luisa HM (2016) Validation of an assay for quantification of alpha-amylase in saliva of sheep. Can J Vet Res 80:197–202

    CAS  PubMed  PubMed Central  Google Scholar 

  • Gatti R, De Palo EF (2011) An update: salivary hormones and physical exercise. Scand J Med Sci Sports 21:157–169

    Article  CAS  PubMed  Google Scholar 

  • Geiss A, Rohleder N, Anton F (2012) Evidence for an association between an enhanced reactivity of interleukin-6 levels and reduced glucocorticoid sensitivity in patients with fibromyalgia. Psychoneuroendocrinology 37:671–684

    Article  CAS  PubMed  Google Scholar 

  • Gerdle B, Ghafouri B, Ernberg M, Larsson B (2014) Chronic musculoskeletal pain: review of mechanisms and biochemical biomarkers as assessed by the microdialysis technique. J Pain Res 7:313–326

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Giltay EJ, Enter D, Zitman FG, Penninx BW, van Pelt J, Spinhoven P, Roelofs K (2012) Salivary testosterone: associations with depression, anxiety disorders, and antidepressant use in a large cohort study. J Psychosom Res 72:205–213

    Article  PubMed  Google Scholar 

  • Goedert M, Nagy JI, Emson PC (1982) The origin of substance P in the rat submandibular gland and its major duct. Brain Res 252:327–333

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez D, Marquina R, Rondon N, Rodriguez-Malaver A, Reyes R (2008) Effects of aerobic exercise on uric acid, total antioxidant activity, oxidative stress, and nitric oxide in human saliva. Res Sport Med 16:128–137

    Article  Google Scholar 

  • González LA, Schwartzkopf-Genswein KS, Caulkett NA, Janzen E, McAllister TA, Fierheller E, Schaefer AL, Haley DB, Stookey JM, Hendrick S (2010) Pain mitigation after band castration of beef calves and its effects on performance, behavior, Escherichia coli, and salivary cortisol. J Anim Sci 88:802–810

    Article  PubMed  CAS  Google Scholar 

  • Goodin BR, Smith MT, Quinn NB, King CD, McGuire L (2012) Poor sleep quality and exaggerated salivary cortisol reactivity to the cold pressor task predict greater acute pain severity in a non-clinical sample. Biol Psychol 91:36–41

    Article  PubMed  PubMed Central  Google Scholar 

  • Greco R, Tassorelli C, Sandrini G, Di Bella P, Buscone S, Nappi G (2008) Role of calcitonin gene-related peptide and substance P in different models of pain. Cephalalgia: an international journal of. Headache 28:114–126

    CAS  Google Scholar 

  • Green TC, Mellor DJ (2011) Extending ideas about animal welfare assessment to include ‘quality of life’ and related concepts. N Z Vet J 59:263–271

    Article  CAS  PubMed  Google Scholar 

  • Griffin J (1986) Well-being: its meaning, measurement, and moral importance. Clarendon paperbacks, pp 10–16

    Google Scholar 

  • Groza P, Zamfir V, Lungu D (1971) Postoperative salivary amylase changes in children. Rev Roum Physiol 8:307–312

    CAS  PubMed  Google Scholar 

  • Guglielminotti J, Dehoux M, Mentré F, Bedairia E, Montravers P, Desmonts JM, Longrois D (2012) Assessment of salivary amylase as a stress biomarker in pregnant patients. Int J Obstet Anesth 21:35–39

    Article  CAS  PubMed  Google Scholar 

  • Häger C, Biernot S, Buettner M, Glage S, Keubler LM, Held N, Bleich EM, Otto K, Müller CW, Decker S, Talbot SR, Bleich A (2017) The Sheep Grimace Scale as an indicator of post-operative distress and pain in laboratory sheep. PLoS One 12(4):e0175839

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Hart KA (2012) The use of cortisol for the objective assessment of stress in animals: pros and cons. Vet J 192:137–139

    Article  CAS  PubMed  Google Scholar 

  • Hernandez CE, Thierfelder T, Svennersten-Sjaunja K, Berg C, Orihuela A, Lidfors L (2014) Time lag between peak concentrations of plasma and salivary cortisol following a stressful procedure in dairy cattle. Acta Vet Scand 9:56–61

    Google Scholar 

  • Hill-Soderlund AL, Mills-Koonce WR, Propper C, Calkins SD, Granger DA, Moore GA, Gariepy JL, Cox MJ (2008) Parasympathetic and sympathetic responses to the strange situation in infants and mothers from avoidant and securely attached dyads. Dev Psychobiol 50:361–376

    Article  PubMed  Google Scholar 

  • Holt-Lunstad J, Birmingham W, Light KC (2011) The influence of depressive symptomatology and perceived stress on plasma and salivary oxytocin before, during and after a support enhancement intervention. Psychoneuroendocrinology 36:1249–1256

    Article  CAS  PubMed  Google Scholar 

  • Horvat-Gordon M, Granger DA, Schwartz EB, Nelson VJ, Kivlighan KT (2005) Oxytocin is not a valid biomarker when measured in saliva by immunoassay. Physiol Behav 84:445–448

    Article  CAS  PubMed  Google Scholar 

  • Huang Y, Liu Z, Liu W, Yin C, Ci L, Zhao R, Yang X (2017) Short communication: salivary haptoglobin and chromogranin A as non-invasive markers during restraint stress in pigs. Res Vet Sci 114:27–30

    Article  CAS  PubMed  Google Scholar 

  • Jafari MJ, Khosrowabadi R, Khodakarim S, Khodagholi F, Mohammadian F (2019) The effects of combined exposure to noise and heat on human salivary cortisol and blood pressure. Int J Occup Saf Ergon 22:1–22

    Article  Google Scholar 

  • Jang MU, Park JW, Kho HS, Chung SC, Chung JW (2011) Plasma and saliva levels of nerve growth factor and neuropeptides in chronic migraine patients. Oral Dis 17:187–193

    Article  PubMed  Google Scholar 

  • Jarvis S, Moinard C, Robson SK, Baxter E, Ormandy E, Douglas AJ, Seckl JR, Russell JA, Lawrence AB (2006) Programming the offspring of the pig by prenatal social stress: neuroendocrine activity and behaviour. Horm Behav 49:68–80

    Article  CAS  PubMed  Google Scholar 

  • Jasim H, Carlsson A, Hedenberg-Magnusson B, Ghafouri B, Ernberg M (2018) Saliva as a medium to detect and measure biomarkers related to pain. Sci Rep 8(1):3220

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jenkins BN, Granger DA, Roemer RJ, Martinez A, Torres TK, Fortier MA (2018) Emotion regulation and positive affect in the context of salivary alpha-amylase response to pain in children with cancer. Pediatr Blood Cancer 65(6):e26973

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kaiser M, Jacobsen S, Andersen PH, Bækbo P, Cerón JJ, Dahl J, Escribano D, Theil PK, Jacobson M (2018) Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome. BMC Vet Res 14(1):334

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kallman TF, Ghafouri B, Bäckryd E (2018) Salivary beta-endorphin and substance P are not biomarkers of neuropathic chronic painpropensity. Heliyon 4(8):e00718

    Article  PubMed  PubMed Central  Google Scholar 

  • Kanno T, Asada N, Yanase H, Iwanaga T, Ozaki T, Nishikawa Y, Iguchi K, Mochizuki T, Hoshino M, Yanaihara N (1999) Salivary secretion of highly concentrated chromogranin A in response to noradrenaline and acetylcholine in isolated and perfused rat submandibular glands. Exp Physiol 84:1073–1083

    Article  CAS  PubMed  Google Scholar 

  • Kennedy B, Dillon E, Mills PJ, Ziegler MG (2001) Catecholamines in human saliva. Life Sci 69:87–99

    Article  CAS  PubMed  Google Scholar 

  • Kikkawa A, Uchida Y, Nakade T, Taguchi K (2003) Salivary secretory IgA concentrations in beagle dogs. Vet Med Sci 65:689–693

    Article  Google Scholar 

  • Klentrou P, Cieslak T, MacNeil M, Vintinner A, Plyley M (2002) Effect of moderate exercise on salivary immunoglobulin A and infection risk in humans. Eur J Appl Physiol 87:153–158

    Article  CAS  PubMed  Google Scholar 

  • Koopmans SJ, van der Staay FJ, Le Floc’h N, Dekker R, van Diepen JT, Jansman AJ (2012) Effects of surplus dietary L-tryptophan on stress, immunology, behavior, and nitrogen retention in endotoxemic pigs. J Anim Sci 90:241–251

    Article  CAS  PubMed  Google Scholar 

  • Kovács L, Kézér FL, Kulcsár-Huszenicza M, Ruff F, Szenci O, Jurkovich V (2016) Hypothalamic-pituitary-adrenal and cardiac autonomic responses to transrectal examination differ with behavioral reactivity in dairy cows. J Dairy Sci 99:7444–7457

    Article  PubMed  CAS  Google Scholar 

  • La Marca-Ghaemmaghami P, La Marca R, Dainese SM, Haller M, Zimmermann R, Ehlert U (2013) The association between perceived emotional support, maternal mood, salivary cortisol, salivary cortisone, and the ratio between the two compounds in response to acute stress in second trimester pregnant women. J Psychosom Res 75:314–320

    Article  PubMed  Google Scholar 

  • Lee T, Shimizu T, Iijima M, Obinata K, Yamashiro Y, Nagasawa S (2006) Evaluation of psychosomatic stress in children by measuring salivary chromogranin A. Acta Paediatr 95:935–939

    Article  PubMed  Google Scholar 

  • Lokesh K, Kannabiran J, Rao MD (2016) Salivary lactate dehydrogenase (LDH)- a novel technique in oral cancer detection and diagnosis. J Clin Diagn Res 10(2):ZC34–ZC37

    CAS  PubMed  PubMed Central  Google Scholar 

  • López-Arjona M, Mateo SV, Manteca X, Escribano D, Cerón JJ, Martínez-Subiela S (2019) Oxytocin in saliva of pigs: an assay for its measurement and changes after farrowing. Domest Anim Endocrinol 70:106384

    Article  PubMed  CAS  Google Scholar 

  • Mach N, Foury A, Kittelmann S, Reigner F, Moroldo M, Ballester M, Esquerré D, Rivière J, Sallé G, Gérard P, Moisan MP, Lansade L (2017) The effects of weaning methods on gut microbiota composition and horse physiology. Front Physiol 8:535

    Article  PubMed  PubMed Central  Google Scholar 

  • MacLean EL, Gesquiere LR, Gee N, Levy K, Martin WL, Carter CS (2018) Validation of salivary oxytocin and vasopressin as biomarkers in domestic dogs. J Neurosci Methods 293:67–76

    Article  CAS  PubMed  Google Scholar 

  • Mandel AL, Peyrot d, Gachons C, Plank KL, Alarcon S, Breslin PA (2010) Individual differences in AMY1 gene copy number, salivary α-amylase levels, and the perception of oral starch. PLoS One 5:e13352

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Marti S, Devant M, Amatayakul-Chantler S, Jackson JA, Lopez E, Janzen ED, Schwartzkopf-Genswein KS (2015) Effect of anti-gonadotropin-releasing factor vaccine and band castration on indicators of welfare in beef cattle. J Anim Sci 93:1581–1591

    Article  CAS  PubMed  Google Scholar 

  • Marti S, Meléndez DM, Pajor EA, Moya D, Heuston CEM, Gellatly D, Janzen ED, Schwartzkopf-Genswein KS (2017) Effect of band and knife castration of beef calves on welfare indicators of pain at three relevant industry ages: II. Chronic pain. J Anim Sci 95:4367–4380

    Article  CAS  PubMed  Google Scholar 

  • Martínez-Miró S, Tecles F, Ramón M, Escribano D, Hernández F, Madrid J, Orengo J, Martínez-Subiela S, Manteca X, Cerón JJ (2019) Causes, consequences and biomarkers of stress in swine: an update. BMC Vet Res 125:227–234

    Google Scholar 

  • Masih T, Dimmock JA, Guelfi KJ (2019) The effect of a single, brief practice of progressive muscle relaxation after exposure to an acute stressor on subsequent energy intake. Stress Health. https://doi.org/10.1002/smi.2891. [In press]

  • Mehta PH, Josephs RA (2010) Testosterone and cortisol jointly regulate dominance: evidence for a dual-hormone hypothesis. Horm Behav 58:898–906

    Article  CAS  PubMed  Google Scholar 

  • Merlot E, Mounier AM, Prunier A (2011) Endocrine response of gilts to various common stressors: a comparison of indicators and methods of analysis. Physiol Behav 102:259–265

    Article  CAS  PubMed  Google Scholar 

  • Miller SC, Kennedy C, DeVoe D, Hickey M, Nelson T, Kogan L (2009) An examination of changes in oxytocin levels in men and women before and after interaction with a bonded dog. Anthrozoös 22(2009):31–42

    Article  Google Scholar 

  • Mitsui S, Yamamoto M, Nagasawa M, Mogi K, Kikusui T, Ohtani N, Ohta M (2011) Urinary oxytocin as a noninvasive biomarker of positive emotion in dogs. Horm Behav 60:239–243

    Article  CAS  PubMed  Google Scholar 

  • Montoya ER, Terburg D, Bos PA, van Honk J (2012) Testosterone, cortisol, and serotonin as key regulators of social aggression: a review and theoretical perspective. Motiv Emot 36:65–73

    Article  PubMed  Google Scholar 

  • Morrison WE, Haas EC, Shaffner DH, Garrett ES, Fackler JC (2003) Noise, stress, and annoyancein a pediatric intensive care unit. Crit Care Med 31:113

    Article  PubMed  Google Scholar 

  • Moya SL, Boyle L, Arkins S, Lynch PB (2006) Effects of husbandry and low-dose lipopolysaccharide challenge on the acute phase response of young pigs, pp 24–30. https://t-stor.teagasc.ie/handle/11019/982. Accessed 13 July 19.

  • Moya D, González LA, Janzen E, Caulkett NA, Fireheller E, Schwartzkopf-Genswein KS (2014) Effects of castration method and frequency of intramuscular injections of ketoprofen on behavioral and physiological indicators of pain in beef cattle. J Anim Sci 92:1686–1697

    Article  CAS  PubMed  Google Scholar 

  • Müller MJ (2011) Helplessness and perceived pain intensity: relations to cortisol concentrations after electrocutaneous stimulation in healthy young men. Biopsychosocial Med 30:5–8

    Google Scholar 

  • Muneta Y, Yoshikawa T, Minagawa Y, Shibahara T, Maeda R, Omata Y (2010) Salivary IgA as a useful non-invasive marker for restraint stress in pigs. Vet Med Sci 72:1295–1300

    Article  Google Scholar 

  • Muneta Y, Minagawa Y, Nakane T, Shibahara T, Yoshikawa T, Omata Y (2011) Interleukin-18 expression in pig salivary glands and salivary content changes during acute immobilization stress. Stress 14:549–556

    Article  CAS  PubMed  Google Scholar 

  • Nam JW, Chung JW, Kho HS, Chung SC, Kim YK (2007) Nerve growth factor concentration in human saliva. Oral Dis 13:187–192

    Article  PubMed  Google Scholar 

  • Nater UM, Rohleder N (2009) Salivary alpha-amylase as a non-invasive biomarker for the sympathetic nervous system: current state of research. Psychoneuroendocrinology 34:486–496

    Article  CAS  PubMed  Google Scholar 

  • Naumova EA, Sandulescu T, Al Khatib P, Thie M, Lee WK, Zimmer S, Arnold WH (2012) Acute short-term mental stress does not influence salivary flow rate dynamics. PLoS One 7:e51323

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ng V, Koh D, Ong HY, Chia SE, Ong CN (1999) Are salivary immunoglobulin A and lysozyme biomarkers of stress among nurses? J Occup Environ Med 41:920–927

    Article  CAS  PubMed  Google Scholar 

  • Ninomiya S, Sato S (2011) The assessment of the effect of presenting a companion’s face picture on social isolation stress using saliva sampling in cow. Anim Sci J 82:787–791

    Article  CAS  PubMed  Google Scholar 

  • Nussbaum MC (2000) Women and human development: the capabilities approach. Cambridge University Press, New York, pp 78–80

    Book  Google Scholar 

  • Obayashi K (2013) Salivary mental stress proteins. Clin Chim Acta 425:196–201

    Article  CAS  PubMed  Google Scholar 

  • Obmiński Z, Wojtkowiak M, Stupnicki R, Golec L, Hackney AC (1997) Effect of acceleration stress on salivary cortisol and plasma cortisol and testosterone levels in cadet pilots. J Physiol Pharmacol 48:193–200

    PubMed  Google Scholar 

  • Odendaal J, Meintjes RA (2003) Neurophysiological correlates of affiliative behaviour between humans and dogs. Vet J 165:296–301

    Article  CAS  PubMed  Google Scholar 

  • Okamoto Y, Ohtani N, Uchiyama H, Ohta M (2009) The feeding behavior of dogs correlates with their responses to commands. J Vet Med Sci 71:1617–1621

    Article  PubMed  Google Scholar 

  • Ooishi Y, Mukai H, Watanabe K, Kawato S, Kashino M (2017) Increase in salivary oxytocin and decrease in salivary cortisol after listening to relaxing slow-tempo and exciting fast-tempo music. PLoS One 12(12):e0189075

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ott S, Soler L, Moons CP, Kashiha MA, Bahr C, Vandermeulen J, Janssens S, Gutiérrez AM, Escribano D, Cerón JJ, Berckmans D, Tuyttens FA, Niewold TA (2014) Different stressors elicit different responses in the salivary biomarkers cortisol, haptoglobin, and chromogranin A in pigs. Res Vet Sci 97:124–128

    Article  CAS  PubMed  Google Scholar 

  • Pang WY, Earley B, Sweeney T, Crowe MA (2006) Effect of carprofen administration during banding or burdizzo castration of bulls on plasma cortisol, in vitro interferon-gamma production, acute-phase proteins, feed intake, and growth. J Anim Sci 84:351–359

    Article  CAS  PubMed  Google Scholar 

  • Pang WY, Earley B, Murray M, Sweeney T, Gath V, Crowe MA (2011) Banding or Burdizzo castration and carprofen administration on peripheral leukocyte inflammatory cytokine transcripts. Res Vet Sci 90:127–132

    Article  CAS  PubMed  Google Scholar 

  • Parfit D (1984) Reasons and persons. Oxford University Press, Oxford, pp 493–502

    Google Scholar 

  • Parris WC, Kambam JR, Naukam RJ, Rama Sastry BV (1990) Immunoreactive substance P is decreased in saliva of patients with chronic back pain syndromes. Anesth Analg 70:63–67

    Article  CAS  PubMed  Google Scholar 

  • Parrott RF, Misson BH (1989) Changes in pig salivary cortisol in response to transport simulation, food and water deprivation, and mixing. Br Vet J 145:501–505

    Article  CAS  PubMed  Google Scholar 

  • Paszynska E, Dmitrzak-Weglarz M, Tyszkiewicz-Nwafor M, Slopien A (2016) Salivary alpha-amylase, secretory IgA and free cortisol as neurobiological components of the stress response in the acute phase of anorexia nervosa. World J Biol Psychiatry 17(4):266–273

    Google Scholar 

  • Peeters M, Sulon J, Beckers J, Ledoux D, Vandeheede M (2011) Comparison between blood serum and salivary cortisol concentrations in horses using an adrenocorticotropic hormone challenge. Equine Vet J 43:487–493

    Article  CAS  PubMed  Google Scholar 

  • Posner EA (2008) Human Welfare, Not Human Rights. Columbia Law Rev 108:1758–1802

    Google Scholar 

  • Pressman SD, Cohen S (2005) Does positive affect influence health? Psychol Bull 131:925–971

    Article  PubMed  Google Scholar 

  • Radaković M, Borozan S, Djelić N, Ivanović S, Miladinović DĆ, Ristanić M, Spremo-Potparević B, Stanimirović Z (2018) Nitroso-oxidative stress, acute phase response, and cytogenetic damage in wistar rats treated with adrenaline. Oxidative Med Cell Longev 21:1805354. eCollection 2018

    Google Scholar 

  • Rai B, Kaur J (2011) Salivary stress markers and psychological stress in simulated microgravity: 21 days in 6° head-down tilt. J Oral Sci 53:103–107

    Article  PubMed  Google Scholar 

  • Rey-Salgueiro L, Martinez-Carballo E, Fajardo P, Chapela MJ, Espiñeira M, Simal-Gandara J (2018) Meat quality in relation to swine well-being after transport and during lairage at the slaughterhouse. Meat Sci 142:38–43

    Article  CAS  PubMed  Google Scholar 

  • Ring RH, Malberg JE, Potestio L, Ping J, Boikess S, Luo B et al (2006) Anxiolytic-like activity of oxytocin in male mice: behavioral and autonomic evidence, therapeutic implications. Psychopharmacology 185(2):218–225

    Article  CAS  PubMed  Google Scholar 

  • Robles TF, Sharma R, Park KS, Harrell L, Yamaguchi M, Shetty V (2012) Utility of a salivary biosensor for objective assessment of surgery-related stress. J Oral Maxillofac Surg 70:2256–2263

    Article  PubMed  PubMed Central  Google Scholar 

  • Rohleder N, Wolf JM, Maldonado EF, Kirschbaum C (2006) The psychosocial stress-induced increase in salivary alpha-amylase is independent of saliva flow rate. Psychophysiology 43:645–652

    Article  PubMed  Google Scholar 

  • Romero-Martínez A, González-Bono E, Lila M, Moya-Albiol L (2013) Testosterone/ cortisol ratio in response to acute stress: a possible marker of risk for marital violence. Soc Neurosci 8:240–247

    Article  PubMed  Google Scholar 

  • Rubio CP, Mainau E, Cerón JJ, Contreras-Aguilar MD, Martínez-Subiela S, Navarro E, Tecles F, Manteca X, Escribano D (2019a) Biomarkers of oxidative stress in saliva in pigs: analytical validation and changes in lactation. BMC Vet Res 15:144

    Article  PubMed  PubMed Central  Google Scholar 

  • Rubio CP, Contreras-Aguilar MD, Quiles A, López-Arjona M, Cerón JJ, Martínez-Subiela S, Hevia ML, Escribano D, Tecles F (2019b) Biomarkers of oxidative stress in saliva of sheep: analytical performance and changes after an experimentally induced stress. Res Vet Sci 123:71–76

    Article  CAS  PubMed  Google Scholar 

  • Ruis MA, Te Brake JH, Engel B, Ekkel ED, Buist WG, Blokhuis HJ, Koolhaas JM (1997) The circadian rhythm of salivary cortisol in growing pigs: effects of age, gender, and stress. Physiol Behav 62:623–630

    Article  CAS  PubMed  Google Scholar 

  • Russell JA, Carroll JM (1999) On the bipolarity of positive and negative affect. Psychol Bull 125:3–30

    Article  CAS  PubMed  Google Scholar 

  • Russell G, Henley D, Leendertz J, Douthwaite J, Wood S, Stevens A, Woltersdorf W, Peeters B, Ruigt G, White A, Velhuis J, Lightman S (2010) Rapid glucocorticoid receptor-mediated inhibition of hypothalamic–pituitary– adrenal ultradian activity in healthy males. J Neurosci 30:6106–6115

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saruta J, Tsukinoki K, Sasaguri K, Ishii H, Yasuda M, Osamura YR, Watanabe Y, Sato S (2005) Expression and localization of chromogranin A gene and protein in human submandibular gland. Cells Tissues Organs 180:237–244

    Article  CAS  PubMed  Google Scholar 

  • Sato F, Kanno T, Nagasawa S, Yanaihara N, Ishida N, Hasegawa T, Iwanaga T (2002) Immunohistochemical localization of chromogranin A in the acinar cells of equine salivary glands contrasts with rodent glands. Cells Tissues Organs 172:29–36

    Article  CAS  PubMed  Google Scholar 

  • Scannapieco FA, Torres G, Levine MJ (1993) Salivary alpha-amylase: role in dental plaque and caries formation. Crit Rev Oral Biol Med 4:301–307

    Article  CAS  PubMed  Google Scholar 

  • Schäffer L, Burkhardt T, Müller-Vizentini D, Rauh M, Tomaske M, Mieth RA, Bauersfeld U, Beinder E (2008) Cardiac autonomic balance in small-for-gestational-age neonates. Am J Physiol Heart Circ Physiol 294:H884–H890

    Article  PubMed  CAS  Google Scholar 

  • Schönbom H, Kassens A, Hopster-Iversen C, Klewitz J, Piechotta M, Martinsson G, Kißler A, Burger D, Sieme H (2015) Influence of transrectal and transabdominal ultrasound examination on salivary cortisol, heart rate, and heart rate variability in mares. Theriogenology 83:749–756

    Article  PubMed  CAS  Google Scholar 

  • Schönreiter S, Huber H, Lohmüller V, Zanella AJ, Unshelm J, Henke J, Erhardt W (1999) [Salivary cortisol as a stress parameter in piglets]. [Article in German]. Tierarztl Prax Ausg G Grosstiere Nutztiere 27:175–179

    PubMed  Google Scholar 

  • Schwab KO, Heubel G, Bartels H (1992) Free epinephrine, norepinephrine and dopamine in saliva and plasma of healthy adults. Eur J Clin Chem Clin Biochem 30:541–544

    CAS  PubMed  Google Scholar 

  • Shin YJ, Shin NS (2016) Evaluation of effects of olfactory and auditory stimulation on separation anxiety by salivary cortisol measurement in dogs. J Vet Sci 17:153–158

    Article  PubMed  PubMed Central  Google Scholar 

  • Shin YJ, Shin NS (2017) Relationship between sociability toward humans and physiological stress in dogs. J Vet Med Sci 79:1278–1283

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shubayev V, Kato K, Myers RR (2010) Cytokines in pain. In: Kruger L, Light AR (eds) Source translational pain research: from mouse to man, Chapter 8. Frontiers in Neuroscience. CRC Press/Taylor & Francis, Boca Raton

    Google Scholar 

  • Sjörs A, Ljung T, Jonsdottir IH (2012) Long-term follow-up of cortisol awakening response in patients treated for stressrelated exhaustion. Br Med J Open 2:e001091

    Google Scholar 

  • Slavish DC, Graham-Engeland JE, Smyth JM, Engeland CG (2015) Salivary markers of inflammation in response to acute stress. Brain Behav Immun 44:253–269

    Article  CAS  PubMed  Google Scholar 

  • Smulders D, Verbeke G, Mormède P, Geers R (2006) Validation of a behavioral observation tool to assess pig welfare. Physiol Behav 89:438–447

    Article  CAS  PubMed  Google Scholar 

  • Soler L, Gutiérrez A, Escribano D, Fuentes M, Cerón JJ (2013) Response of salivary haptoglobin and serum amyloid A to social isolation and short road transport stress in pigs. Res Vet Sci 95:298–302

    Article  CAS  PubMed  Google Scholar 

  • Sordillo LM, Aitken SL (2009) Impact of oxidative stress on the health and immune function of dairy cattle. Vet Immunol Immunopathol 128:104–109

    Article  CAS  PubMed  Google Scholar 

  • Spiropoulos K, Lymberopoulos D, Garantziotis G, Gogos C (1993) Salivary immunoglobulin A production in chronic bronchitis patients given an orally administered bacterial extract. Respiration 60:313–318

    Article  CAS  PubMed  Google Scholar 

  • Spruijt BM, van den Bos R, Pijlman FTA (2001) A concept of welfare based on reward evaluating mechanisms in the brain: antic- ipatory behaviour as an indicator for the state of reward systems. Appl Anim Behav Sci 72:145–171

    Article  PubMed  Google Scholar 

  • Svobodová I, Chaloupková H, Končel R, Bartoš L, Hradecká L, Jebavý L (2014) Cortisol and secretory immunoglobulin A response to stress in German shepherd dogs. PLoS One 9(3):e90820

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Symons F, ElGhazi I, Reilly BG, Barney CC, Hanson L, Panoskaltsis-Mortari A, Armitage IM, Wilcox GL (2015) Can biomarkers differentiate pain and no pain subgroups of nonverbal children with cerebral palsy? A preliminary investigation based on noninvasive saliva sampling. Pain Med 16:249–256

    Article  PubMed  Google Scholar 

  • Syrjänen S, Piironen P, Yli-Urpo A (1984) Salivary content of patients with subjective symptoms resembling galvanic pain. Oral Surg Oral Med Oral Pathol 58:387–393

    Article  PubMed  Google Scholar 

  • Takatsuji K, Sugimoto Y, Ishizaki S, Ozaki Y, Matsuyama E, Yamaguchi Y (2008) The effects of examination stress on salivary cortisol, immunoglobulin A, and chromogranin A in nursing students. Biomed Res 29:221–224

    Article  CAS  PubMed  Google Scholar 

  • Takiyyuddin MA, Brown MR, Dinh TQ, Cervenka JH, Braun SD, Parmer RJ, Kennedy B, O’Connor DT (1994) Sympatho–adrenal secretion in humans: factors governing catecholamine and storage vesicle peptide co-release. J Auton Pharmacol 14:187–200

    Article  CAS  PubMed  Google Scholar 

  • Tecles F, Tvarijonaviciute A, De Torre C, Carrillo JM, Rubio M, García M, Cugat R, Cerón JJ (2016) Total esterase activity in human saliva: validation of an automated assay, characterization and behaviour after physical stress. Scand J Clin Lab Invest 76:324–330

    Article  CAS  PubMed  Google Scholar 

  • Tecles F, Contreras-Aguilar MD, Martínez-Miró S, Tvarijonaviciute A, Martínez-Subiela S, Escribano D, Cerón JJ (2017) Total esterase measurement in saliva of pigs: validation of an automated assay, characterization and changes in stress and disease conditions. Res Vet Sci 114:170–176

    Article  CAS  PubMed  Google Scholar 

  • Tecles F, Escribano D, Contreras-Aguilar MD, Rubio CP, Szczubiał M, Cerón JJ, Dąbrowski R, Tvarijonaviciute A (2018) Evaluation of adenosine deaminase in saliva and serum, and salivary α-amylase, in canine pyometra at diagnosis and after ovariohysterectomy. Vet J 236:102–110

    Article  CAS  PubMed  Google Scholar 

  • Toda M, Den R, Nagasawa S, Kitamura K, Morimoto K (2007) Relationship between lifestyle score and salivary stress markers cortisol and chromogranin A. Arch Environ Occup Health 60:266–269

    Article  Google Scholar 

  • Tóthová L, Kamodyová N, Červenka T, Celec P (2015) Salivary markers of oxidative stress in oral diseases. Front Cell Infect Microbiol 5:73

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ulmer-Yaniv A, Djalovski A, Priel A, Zagoory-Sharon O, Feldman R (2018) Maternal depression alters stress and immune biomarkers in mother and child. Depress Anxiety 35:1145–1157

    Article  PubMed  Google Scholar 

  • Vahedi M, Mazdeh M, Hajilooi M, Farhadian M, Barakian Y, Sadr P (2018) The Relationship between salivary alpha amylase activity and score of McGill pain questionnaire in patients with tension type headache. Basic Clin Neurosci 9:59–64

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • van der Staay FJ, de Groot J, Schuurman T, Korte SM (2008) Repeated social defeat in female pigs does not induce neuroendocrine symptoms of depression, but behavioral adaptation. Physiol Behav 93:453–460

    Article  PubMed  CAS  Google Scholar 

  • van Stegeren AH, Wolf OT, Kindt M (2008) Salivary alpha amylase and cortisol responses to different stress tasks: impact of sex. Int J Psychophysiol 69:33–40

    Article  PubMed  Google Scholar 

  • Walker LJ, Aldhous MC, Drummond HE, Smith BR, Nimmo ER, Arnott ID, Satsangi J (2004) Anti-Saccharomyces cerevisiae antibodies (ASCA) in Crohn’s disease are associated with disease severity but not NOD2/CARD15 mutations. Clin Exp Immunol 135:490–496

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Weisman O, Zagoory-Sharon O, Feldman R (2012) Intranasal oxytocin administration is reflected in human saliva. Psychoneuroendocrinology 37:1582–1586

    Article  CAS  PubMed  Google Scholar 

  • Wetherell MA, Hyland ME, Harris JE (2004) Secretory immunoglobulin A reactivity to acute and cumulative acute multi-tasking stress: relationships between reactivity and perceived workload. Biol Psychol 66:257–270

    Article  PubMed  Google Scholar 

  • White-Traut R, Watanabe K, Pournajafi-Nazarloo H, Schwertz D, Bell A, Carter CS (2009) Detection of salivary oxytocin levels in lactating women. Dev Psychobiol 51:367–373

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Windle RJ, Kershaw YM, Shanks N, Wood SA, Lightman SL, Ingram CD (2004) Oxytocin attenuates stress-induced c-fos mRNA expression in specific forebrain regions associated with modulation of hypothalamo-pituitary-adrenal activity. J Neurosci 24(12):2974–2982

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Woolston JL, Gainfredi S, Gertner JM, Paugus JA, Mason JW (1983) Salivary cortisol: a non traumatic sampling technique for assaying cortisol dynamics. J Am Acad Child Psychiatry 22:474–476

    Article  CAS  PubMed  Google Scholar 

  • Wozniak KM, Rojas C, Wu Y, Slusher BS (2012) The role of glutamate signaling in pain processes and its regulation by GCP II inhibition. Curr Med Chem 19:1323–1334

    Article  CAS  PubMed  Google Scholar 

  • Yeates JW, Main DCJ (2008) Assessment of positive welfare: a review. Vet J 175:293–300

    Article  CAS  PubMed  Google Scholar 

  • Yoto A, Fukui N, Kaneda C, Torita S, Goto K, Nanjo F, Yokogoshi H (2018) Black tea aroma inhibited increase of salivary chromogranin-A after arithmetic tasks. J Physiol Anthropol 37(1):3

    Article  PubMed  PubMed Central  Google Scholar 

  • Yun J, Björkman S, Pöytäkangas M, Peltoniemi O (2017) The effects of ovarian biopsy and blood sampling methods on salivary cortisol and behaviour in sows. Res Vet Sci 114:80–85

    Article  CAS  PubMed  Google Scholar 

  • Zidverc-Trajkovic J, Stanimirovic D, Obrenovic R, Tajti J, Vécsei L, Gardi J, Németh J, Mijajlovic M, Sternic N, Jankovic L (2009) Calcitonin gene-related peptide levels in saliva of patients with burning mouth syndrome. J Oral Pathol Med 38:29–33

    Article  PubMed  Google Scholar 

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Acknowledgements

Damian Escribano has a post-doctoral fellowshisp (IJC2018-035105-I) “Juan de la Cierva Incorporación” supported by the “Ministerio de Economía y Competitividad”, Spain.

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  1. Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain

    Damián Escribano & Fernando Tecles

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  1. Damián Escribano
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  2. Fernando Tecles
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Correspondence to Damián Escribano .

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  1. Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain

    Asta Tvarijonaviciute

  2. Interlab-UMU, University of Murcia, Murcia, Spain

    Silvia Martínez-Subiela

  3. Department of Oral Medicine, Faculty of Medicine and Dentistry, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain

    Pia López-Jornet

  4. MED - Mediterranean Institute for Agriculture Environment and Development, IIFA - Instituto de Investigação e Formação Avançada, University of Évora, Évora, Portugal

    Elsa Lamy

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Escribano, D., Tecles, F. (2020). Salivary Biomarkers in Welfare Studies. In: Tvarijonaviciute, A., Martínez-Subiela, S., López-Jornet, P., Lamy, E. (eds) Saliva in Health and Disease. Springer, Cham. https://doi.org/10.1007/978-3-030-37681-9_14

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