Abstract
This contribution aims to show the anatomical and physiological characteristics of the salivary glands as entity for the production of saliva, and to present the composition of the saliva fluid as a protective medium for the mouth, the start of digestion and as diagnostic medium. All this from a comparative point of view between humans and animals.
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References
Amado FML, Ferreira RP, Vitorino R (2013) One decade of salivary proteomics: current approaches and outstanding challenges. Clin Biochem 46(6, SI):506–517. https://doi.org/10.1016/j.clinbiochem.2012.10.024
Anderson LC, Martin DJ, Phillips DL, Killpack KJ, Bone SE, Rahimian R (2006) The influence of gender on parasympathetic vasodilatation in the submandibular gland of the rat. Exp Physiol 91(2):435–444. https://doi.org/10.1113/expphysiol.2005.032730
Asari M, Miura K, Ichihara N, Nishita T, Amasaki H (2000) Distribution of carbonic anhydrase isozyme VI in the developing bovine parotid gland. Cells Tissues Organs 167(1):18–24. https://doi.org/10.1159/000016762
Asking B, Gjorstrup P (1987) Synthesis and secretion of amylase in the rat parotid gland following autonomic nerve stimulation in vivo. Acta Physiol Scand 130(3):439–445. https://doi.org/10.1111/j.1748-1716.1987.tb08160.x
Barranco T, Tvarijonaviciute A, Tecles F, Carrillo JM, Sanchez-Resalt C, Jimenez-Reyes P et al (2018) Changes in creatine kinase, lactate dehydrogenase and aspartate aminotransferase in saliva samples after an intense exercise: a pilot study. J Sports Med Phys Fitness 58(6):910–916. https://doi.org/10.23736/S0022-4707.17.07214-0
Baum B (1993) Principles of saliva secretion. Ann N Y Acad Sci 20(694):17–23
Baum BJ, Wellner RB (1999) Receptors in salivary glands. In: Garrett JR, Ekstrom J, Anderson LC (eds) Neural mechanisms of salivary secretion, Frontiers in oral biology. Karger, Basel, pp 44–58
Beltzer EK, Fortunato CK, Guaderrama MM, Peckins MK, Garramone BM, Granger DA (2010) Salivary flow and alpha-amylase: collection technique, duration, and oral fluid type. Physiol Behav 101(2):289–296. https://doi.org/10.1016/j.physbeh.2010.05.016
Biesbrock A, Reddy M, Levine M (1991) Interaction of salivary mucin-secre- tory immunoglobulin A complex with mucosal pathogens. Infect Immun 59(10):3492–3497
Blaschko H, Comline RS, Schneider FH, Silver M, Smith AD (1967) Secretion of a chromaffin granule protein, chromogranin, from the adrenal gland after splanchnic stimulation. Nature 215:58–59
Brandtzaeg P (1998) Synthesis and secretion of human salivary immunoglobulins. In: Garrett J, Ekstrom J, Anderson LC (eds) Glandular mechanisms of salivary secretion. Karger, Basel, pp 167–199
Butterworth PJ, Warren FJ, Ellis PR (2011) Human α-amylase and starch digestion: an interesting marriage. Starch/Staerke 63(7):395–405. https://doi.org/10.1002/star.201000150
Büttler RM, Bagci E, Brand HS, den Heijer M, Blankenstein MA, Heijboer AC (2018) Testosterone, androstenedione, cortisol and cortisone levels in human unstimulated, stimulated and parotid saliva. Steroids 138:26–34. https://doi.org/10.1016/j.steroids.2018.05.013
Cappai MG, Dall’Aglio C, Sander SJ, Ratert C, Dimauro C, Pinna W, Kamphues J (2016) Different physical forms of one diet fed to growing pigs induce morphological changes in mandubular glands and local leptin (Ob) production and receptor (ObR) expression. J Anim Physiol Anim Nutr 100(6):1067–1072. https://doi.org/10.1111/jpn.12478
Carpenter GH (2013) The secretion, components, and properties of saliva. Annu Rev Food Sci Technol 4(February 2013):267–276. https://doi.org/10.1146/annurev-food-030212-182700
Carpenter GH, Garrett JR, Hartley RH, Proctor GB (1998) The influence of nerves on the secretion of immunoglobulin A into submandibular saliva in rats. J Physiol 512(2):567–573. https://doi.org/10.1111/j.1469-7793.1998.567be.x
Castle D, Castle A (1998) Intracellular transport and secretion of salivary proteins. Crit Rev Oral Biol Med 9(1):4–22. https://doi.org/10.1177/10454411980090010301
Castle J, Jamieson J, Palade G (1975) Secretion granules of the rabbit parotid gland. Isolation, subfractionation, and characterization of the membrane and content subfractions. J Cell Biol 64(1):182–210. https://doi.org/10.1083/jcb.64.1.182
Chimenos Kustner E, Ribera Uribe M, López López J (2012) Gerodontología (1a; S. E. de Gerontología, ed.). Santiago de Compostela, España: La Ibérica
Choe JK, Khan-Dawood FS, Yusoff-Dawood M (1983) Progesterone and estradiol in the saliva and plasma during the menstrual cycle. Am J Obstet Gynecol 147(5):557–562. https://doi.org/10.1016/0002-9378(83)90016-9
Contreras-Aguilar MD, Tecles F, Martínez-Subiela S, Escribano D, Bernal L, Cerón JJ (2017) Detection and measurement of alpha- amylase in canine saliva and changes after an experimentally induced sympathetic activation. BMC Vet Res 13(266):1–6. https://doi.org/10.1186/s12917-017-1191-4
Contreras-Aguilar MD, Escribano D, Martín-cuervo M, Tecles F, Cerón JJ (2018a) Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study. BMC Vet Res 14(156):1–7
Contreras-Aguilar MD, Escribano D, Martínez-subiela S, Martínez-Miró S, Cerón JJ, Tecles F (2018b) Changes in alpha-amylase activity , concentration and isoforms in pigs after an experimental acute stress model: an exploratory study. BMC Vet Res 14(256):1–8
Contreras-Aguilar M, Escribano D, Quiles A, López-Arjona M, Cerón J, Martínez-Subiela S et al (2018c) Evaluation of new biomarkers of stress in saliva of sheep. Animal 13(6):1–9. https://doi.org/10.1017/S1751731118002707
Contreras-Aguilar M, Escribano D, Martínez-Subiela S, Martín-Cuervo M, Lamy E, Tecles F, Cerón J (2019) Changes in saliva analytes in equine acute abdominal disease: a sialochemistry approach. BMC Vet Res 6:1–9
Davis R, Anson B, Budinger J, Kurth L (1956) Surgical anatomy of the facial nerve and parotid gland based upon a study of 350 cervicofacial halves. Surg Gynecol Obstet 102(4):384–412
de Sousa-Pereira P, Cova M, Abrantes J, Ferreira R, Trindade F, Barros A et al (2015) Cross-species comparison of mammalian saliva using an LC-MALDI based proteomic approach. Proteomics 15(9):1598–1607. https://doi.org/10.1002/pmic.201400083
Debono M, Harrison RF, Whitaker MJ, Eckland D, Arlt W, Keevil BG, Ross RJ (2016) Salivary cortisone reflects cortisol exposure under physiological conditions and after hydrocortisone. J Clin Endocrinol Metab 101(4):1469–1477. https://doi.org/10.1210/jc.2015-3694
DeNigris SJ, Hamosh M, Kasbekar DK, Lee TC, Hamosh P (1988) Lingual and gastric lipases: species differences in the origin of prepancreatic digestive lipases and in the localization of gastric lipase. Biochim Biophys Acta Lipids Lipid Metab 959(1):38–45. https://doi.org/10.1016/0005-2760(88)90147-6
Edgar WM (1990) Saliva and dental health. Clinical implications of saliva: report of a consensus meeting. Br Dent J 169(3–4):96–98. https://doi.org/10.1038/sj.bdj.4807284
Edgar M, Dawes C, O’Mullane D (2012) In: Edgar M, Dawes C, O’Mullane D (eds) Saliva and oral health, vol 56, 4th edn. https://doi.org/10.1590/S0104-42302010000100001
Ekström J (1999) Role of nonadrenergic, noncholinergic autonomic transmitters in salivary glandular activities in vivo. In: Garrett J, Ekström J, Anderson L (eds) Neural mechanisms of salivary gland secretion, Frontiers in oral biology, 11th edn. Karger, Basel, pp 94–130
Ekström J, Garrett J, Månsson B, Rowley P, G T (1989) Depletion of large dense-cored vesicles from parasympathetic nerve-terminals in rat parotid-glands after prolonged stimulation of the auriculotemporal nerve. Regul Pept 25(1):61–67. https://doi.org/10.1016/0167-0115(89)90248-6
Emilio Savastano L, Elizabeth Castro A, Rene Fitt M, Fredensborg Rath M, Eduardo Romeo H, Maris Munoz E (2010) A standardized surgical technique for rat superior cervical ganglionectomy. J Neurosci Methods 192(1):22–33. https://doi.org/10.1016/j.jneumeth.2010.07.007
Emmelin N (1987) Nerve interactions in salivary glands. J Dent Res 66(2):509–517. https://doi.org/10.1177/00220345870660022101
Emmelin N, Engstrom J (1960) Effect of sympathetic denervation on the sensitivity of the submaxillary gland to stimulating agents. J Physiol Lond 153(1):9–16. https://doi.org/10.1113/jphysiol.1960.sp006515
Feldstein JB, Silverman DN (1984) Purification and characterization of carbonic-anhidrase from the saliva of the rat. J Biol Chem 259(9):5447–5453
Fernley RT, Coghlan JP, Wright RD (1988) Purification and characterization of a high-Mr carbonic-anhydrase from sheep parotid-gland. Biochem J 249(1):201–207. https://doi.org/10.1042/bj2490201
Ferreira JN, Hoffman MP (2013) Interactions between developing nerves and salivary glands. Organogenesis 9(3):199–205
Funaki H, Yamamoto T, Koyama Y, Kondo D, Yaoita E, Kawasaki K et al (1998) Localization and expression of AQP5 in cornea, serous salivary glands, and pulmonary epithelial cells. Am J Phys Cell Phys 275(4):C1151–C1157
Greabu M, Battino M, Mohora M, Totan A, Didilescu A, Spinu T (2009) Saliva – a diagnostic window to the body, both in health and in disease. J Med Life 2(2):124–132
Gresz V, Kwon TH, Hurley PT, Varga G, Zelles T, Nielsen S et al (2001) Identification and localization of aquaporin water channels in human salivary glands. Am J Physiol Gastrointest Liver Physiol 281(1):G247–G254
Groschl M, Rauh M, Wagner R, Neuhuber W, Metzler M, Tamguney G et al (2001) Identification of leptin in human saliva. J Clin Endocrinol Metab 86(11):5234–5239. https://doi.org/10.1210/jc.86.11.5234
Groschl M, Topf HG, Bohlender J, Zenk J, Klussman S, Dotsch J et al (2005) Identification of ghrelin in human saliva: production by the salivary glands and potential role in proliferation of oral keratinocytes. Clin Chem 51(6):997–1006. https://doi.org/10.1373/clinchem.2004.040667
Henskens YMC, Vandervelden U, Veerman ECI, Amerongen AVN (1993) Protein, albumin and cystatin concentrations in saliva of healthy-subjects and of patients with gingivitis or periodontitis. J Periodontal Res 28(1):43–48. https://doi.org/10.1111/j.1600-0765.1993.tb01049.x
Humphrey SP, Williamson RT (2001) A review of saliva: normal composition, flow, and function. J Prosthet Dent 85(2):162–169
Huq NL, Cross KJ, Ung M, Reynolds EC (2005) A review of protein structure and gene organisation for proteins associated with mineralised tissue and calcium phosphate stabilisation encoded on human chromosome 4. Arch Oral Biol 50(7):599–609. https://doi.org/10.1016/j.archoralbio.2004.12.009
Iontcheva I, Oppenheim FG, Troxler RF (1997) Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J Dent Res 76(3):734–743. https://doi.org/10.1177/00220345970760030501
Kahle W, Frotscher M, Gerhard S (2010) Color atlas of human anatomy: nerve system and sensory organs, vol 3. Theime Medical, Stuttgart
Kaufman E, Lamster IB (2000) Analysis of saliva for periodontal sis of saliva may offer a cost-effective approach to assessment of periodontal disease in large populations. J Clin Periodontol C Munksgaard 27(27):453–465
Kaufman E, Lamster I (2002) The diagnosis application of saliva: a review. Crit Rev Oral Biol Med 13(2):197–212
Kawasaki K, Weiss KM (2003) Mineralized tissue and vertebrate evolution: the secretory calcium-binding phosphoprotein gene cluster. Proc Natl Acad Sci U S A 100(7):4060–4065. https://doi.org/10.1073/pnas.0638023100
Kim YB, Yang BH, Piao ZG, Oh SB, Kim JS, Park K (2003) Expression of Na+/HCO3- cotransporter and its role in pH regulation in mouse parotid acinar cells. Biochem BIiophys Res Commun 304(4):593–598. https://doi.org/10.1016/S0006-291X(03)00632-6
Kobashi M, Ichikawa H, Kobashi M, Funahashi M, Mitoh Y, Matsuo R (2005) The origin of sensory nerve fibers that innervate the submandibular salivary gland in the rat. Brain Res 1060(1–2):184–187. https://doi.org/10.1016/j.brainres.2005.08.012
Lamy E, da Costa G, Santos R, e Silva F, Potes J, Pereira A et al (2009) Sheep and goat saliva proteome analysis: a useful tool for ingestive behavior research? Physiol Behav 98(4):393–401. https://doi.org/10.1016/j.physbeh.2009.07.002
Lindner A, Marx S, Kissenbeck S, Mosen H (2000) Saliva collection and relationship between lactate concentration in blood and saliva of exercising horses. J Equine Vet Sci 20(1):52–54. https://doi.org/10.1016/S0737-0806(00)80322-6
Lundberg J, Anggård A, Fahrenkrug J (1981) Complementary role of vasoactive intestinal polypeptide (VIP) and acetylcholine for cat submandibular gland blood flow and secretion. II effects of cholinergic antagonists and VIP antiserum. Acta Physiol Scand 113(3):329–336. https://doi.org/10.1111/j.1748-1716.1981.tb06903.x
Luo X, Choi JY, Ko SBH, Pushkin A, Kurtz I, Ahn W et al (2001) HCO3- salvage mechanisms in the submandibular gland acinar and duct cells. J Biol Chem 276(13):9808–9816. https://doi.org/10.1074/jbc.M008548200
Mandel ID (1989) The role of saliva in maintaining oral homeostasis. J Am Dent Assoc 119(2):298–304. https://doi.org/10.14219/jada.archive.1989.0211
Marchetti P, Benzi L, Masoni A, Cecchetti P, Giannarelli R, Di Cianni G et al (1986) Salivary insulin concentrations in type 2 (non-insulin-dependent) diabetic patients and obese non-diabetic subjects: relationship to changes in plasma insulin levels after an oral glucose load. Diabetologia 29(10):695–698. https://doi.org/10.1007/BF00870278
Mathison R, Davison J, Befus A (1994) Neuroendocrine regulation of inflammation and tissue repair by submandibular gland factors. Immunol Today 15(11):527–532. https://doi.org/10.1016/0167-5699(94)90209-7
Matsuo R (2000) Role of saliva in the maintenance of taste sensitivity. Crit Rev Oral Biol Med 11(2):216–229. https://doi.org/10.1177/10454411000110020501
Matsuzaki T, Suzuki T, Koyama H, Tanaka S, Takata K (1999) Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal glands: immunolocalization and effect of secretory stimulation. Cell Tissue Res 295(3):513–521. https://doi.org/10.1007/s004410051257
McDonald RE, Fleming RI, Beeley JG, Bovell DL, Lu JR, Zhao X et al (2009) Latherin: a surfactant protein of horse sweat and saliva. PLoS One 4(5):1–12. https://doi.org/10.1371/journal.pone.0005726
Mega J, McGhee J, Kiyono H (1992) Cytokine- and Ig-producing T cells in mucosal effector tissues: analysis of IL-5- and IFN-gamma-producing T cells, T cell receptor expression, and IgA plasma cells from mouse salivary gland-associated tissues. J Immunol 148(7):2030–2039
Melvin JE, Yule D, Shuttleworth T, Begenisich T (2005) Regulation of fluid and electrolyte secretion in salivary gland acinar cells. Annu Rev Physiol 67(1):445–469. https://doi.org/10.1146/annurev.physiol.67.041703.084745
Messenger B, Clifford MN, Morgan LM (2003) Glucose-dependent insulinotropic polypeptide and insulin-like immunoreactivity in saliva following sham-fed and swallowed meals. J Endocrinol 177(3):407–412. https://doi.org/10.1677/joe.0.1770407
Mizuta K, Karita K, Izumi H (2000) Parasympathetic reflex vasodilatation in rat submandibular gland. Am J Physiol Regul Integr Comp Physiol 279(2):R677–R683
Moller K, Benz D, Perrin D, Soling HD (1996) The role of protein kinase C in carbachol-induced and of cAMP-dependent protein kinase in isoproterenol-induced secretion in primary cultured guinea pig parotid acinar cells. Biochem J 314(1):181–187
Murakami M, Ohtake T, Dorschner RA, Gallo RL (2002) Cathelicidin antimicrobial peptides are expressed in salivary glands and saliva. J Dent Res 81(12):845–850. https://doi.org/10.1177/154405910208101210
Nater UM, Rohleder N (2009) Salivary alpha-amylase as a non-invasive biomarker for the sympathetic nervous system: current state of research. Psychoneuroendocrinology 34(4):486–496. https://doi.org/10.1016/j.psyneuen.2009.01.014
Niada S, Ferreira LM, Arrigoni E, Addis A, Campagnol M, Broccaioli E, Brini AT (2013) Porcine adipose-derived stem cells from buccal fat pad and subcutaneous adipose tissue for future preclinical studies in oral surgery. Stem Cell Res Ther 4:148. https://doi.org/10.1186/scrt359
Oconnor DT (1983) Chromogranin - widespread immunoreactivity in polypeptide hormone producing tissues and in serum. Regul Pept 6(3):263–280. https://doi.org/10.1016/0167-0115(83)90145-3
Perogamvros I, Aarons L, Miller AG, Trainer PJ, Ray DW (2011) Corticosteroid-binding globulin regulates cortisol pharmacokinetics. Clin Endocrinol 74(1):30–36. https://doi.org/10.1111/j.1365-2265.2010.03897.x
Proctor G, Carpenter G (2002) Neural control of salivary secretory IgA secretion. In: Clow A, Hucklebridge F (eds) Neurobiology of the immune system. Academic, San Diego, pp 187–212
Proctor GB, Carpenter GH (2007) Regulation of salivary gland function by autonomic nerves. Auton Neurosci Basic Clin 133(1):3–18. https://doi.org/10.1016/j.autneu.2006.10.006
Roth G, Calmes R (1981) Salivary glands and saliva. In: Mosby C (ed) Oral biology. CV Mosby, St Louis, pp 196–326
Sandoval J (1998) Tratado de Anatomía Veterinaria. Tomo III: Cabeza y Sistemas viscerales, 3rd edn. Imprenta Sorles, León
Saruta J, Tsukinoki K, Sasaguri K, Ishii H, Yasuda M, Osamura YR et al (2005) Expression and localization of chromogranin A gene and protein in human submandibular gland. Cells Tissues Organs 180(4):237–244. https://doi.org/10.1159/000088939
Sato F, Kanno T, Nagasawa S, Yanaihara N, Ishida N, Hasegawa T, Iwanaga T (2002) Immunohistochemical localization of chromogranin A in the acinar cells of equine salivary glands contrasts with rodent glands. Cells Tissues Organs 172(1):29–36. https://doi.org/10.1159/000064389
Segawa A, Yamashina S (1998) The dynamics of exocytosis of preformed secretory granules from acini in rat salivary glands. In: Garrett J, Ekström J, Anderson L (eds) Glandular mechanisms of salivary secretion, Frontiers in oral biology, 10th edn. Karger, Basel, pp 80–100
Shimojo M, Ricketts ML, Petrelli MD, Moradi P, Johnson GD, Bradwell AR et al (1997) Type 2 in human mineralocorticoid target tissues: evidence for nuclear localization. Society 138(3):1305–1311
Slomiany BL, Murty VLN, Piotrowski J, Slomiany A (1996) Salivary mucins in oral mucosal defense. Gen Pharmacol 27(5):761–771. https://doi.org/10.1016/0306-3623(95)02050-0
Tamarin A (1966) Myoepithelium of rat submaxillary gland. J Ultrastruct Res 16(3–4):320–338. https://doi.org/10.1016/S0022-5320(66)80066-7
Thie NMR, Kato T, Bader G, Montplaisir JY, Lavigne GJ (2002) The significance of saliva during sleep and the relevance of oromotor movements. Sleep Med Rev 6(3):213–227. https://doi.org/10.1053/smrv.2001.0183
Van Faassen M, Bischoff R, Kema IP (2017) Relationship between plasma and salivary melatonin and cortisol investigated by LC-MS/MS. Clin Chem Lab Med 55(9):1340–1348. https://doi.org/10.1515/cclm-2016-0817
Vázquez-Autón J, Gil-Cano F, Latorre-Reviriego R, Ramírez-Zarzosa G, López-Albors O, Ayala-Florenciano M et al (2002) In: Marín D (ed) Manual de Prácticas de Anatomía Veterinaria: Sistemas Viscerales, 1st edn. Diego Marín, Murcia
Velayos J, Santana H (2007) Anatomía de la Cabeza para odontólogos (4a). Médica panamericana, Madrid
Vining RF, Mcginley RA, Symons RG (1983) Hormones in saliva: mode if entry and consequent implication for clinical interpretation. Clin Chem 29(10):1752–1756
Young J, Cook D, Lennep E, Roberts M (1987) Secretion by the major salivary glands. In: Johnson L (ed) Physiology of the gastrointestinal tract. Raven Press, New York, pp 773–816
Zanetti M (2005) The role of cathelicidins in the innate host defenses of mammals. Curr Issues Mol Biol 7:179–196
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Contreras-Aguilar, M.D., Gómez-García, F. (2020). Salivary Glands’ Anatomy and Physiology. In: Tvarijonaviciute, A., Martínez-Subiela, S., López-Jornet, P., Lamy, E. (eds) Saliva in Health and Disease. Springer, Cham. https://doi.org/10.1007/978-3-030-37681-9_1
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