The Microbial Communities of the East Mediterranean Sea Mud Volcanoes and Pockmarks

Part of the Springer Oceanography book series (SPRINGEROCEAN)


Mud volcanoes and pockmarks are sites which methane and other gases seep out towards the overlying water. This supply and the microbiologically-mediated processes of these cold seeps can be the basis of unique microbial habitats. Although mud volcanoes and pockmarks vary in the seepage rate and chemical composition of their outflows, the communities found in geographically adjacent systems seem to harbor comparable microbial communities. For the East Mediterranean Sea, only sporadic data exist on its major mud volcanoes and pockmarks collected through a few oceanographic cruises; however, further the knowledge of these systems should be gained by microbiological and sequence-based investigations.


  1. Amann R, Ludwig W, Schleifer K (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143–169Google Scholar
  2. Amend AS, Oliver TA, Amaral-Zettler LA, Boetius A et al (2013) Macroecological patterns of marine bacteria on a global scale. J Biogeogr 40:800–811CrossRefGoogle Scholar
  3. Bienhold C, Zinger L, Boetius A, Ramette A (2016) Diversity and biogeography of bathyal and abyssal seafloor bacteria. PLoS ONE 11:e0148016CrossRefGoogle Scholar
  4. Boetius A, Wenzhöfer F (2013) Seafloor oxygen consumption fueled by methane from cold seeps. Nat Geosci 6:725–734CrossRefGoogle Scholar
  5. Cita MB, Ryan WBF, Paggi L (1981) Prometheus mud-breccia: An example of shale diapirism in the western Mediterranean ridge. Ann Geol Pays Hellen 30:543–570Google Scholar
  6. Hatzenpichler R, Connon SA, Goudeau D, Malmstrom RR, Woyke T, Orphan VJ (2016) Visualizing in situ translational activity for identifying and sorting slow-growing archaeal—bacterial consortia. Proc Natl Acad Sci USA 113:E4069–E4078CrossRefGoogle Scholar
  7. Heijs SK, Haese RR, Van Der Wielen PWJJ, Forney LJ, Van Elsas JD (2007) Use of 16S rRNA gene based clone libraries to assess microbial communities potentially involved in anaerobic methane oxidation in a Mediterranean cold seep. Microb Ecol 53:384–398CrossRefGoogle Scholar
  8. Heijs SK, Laverman AM, Forney LJ, Hardoim PR, Van Elsas JD (2008) Comparison of deep-sea sediment microbial communities in the eastern Mediterranean. FEMS Microbiol Ecol 64:362–377CrossRefGoogle Scholar
  9. Heijs SK, Sinninghe Damsté JS, Forney LJ (2005) Characterization of a deep-sea microbial mat from an active cold seep at the Milano mud volcano in the eastern Mediterranean Sea. FEMS Microbiol Ecol 54:47–56CrossRefGoogle Scholar
  10. Knittel K, Boetius A (2009) Anaerobic oxidation of methane: progress with an unknown process. Ann Rev Microbiol 63:311–334CrossRefGoogle Scholar
  11. Kormas KA, Meziti A, Dahlmann A, De Lange GJ, Lykousis V (2008) Characterization of methanogenic and prokaryotic assemblages based on mcrA and 16S rRNA gene diversity in sediments of the Kazan mud volcano (Mediterranean Sea). Geobiology 6:450–460CrossRefGoogle Scholar
  12. Omoregie EO, Mastalerz V, de Lange G, Straub KL et al (2008) Biogeochemistry and community composition of iron- and sulfur-precipitating microbial mats at the Chefren mud volcano (Nile Deep Sea Fan, eastern Mediterranean). Appl Environ Microbiol 74:3198–3215CrossRefGoogle Scholar
  13. Omoregie EO, Niemann H, Mastalerz V, de Lange GJ et al (2009) Microbial methane oxidation and sulfate reduction at cold seeps of the deep eastern Mediterranean Sea. Mar Geol 261:114–127CrossRefGoogle Scholar
  14. Pachiadaki MG, Kallionaki A, Shlmann D, De Lange A, Kormas KJ (2011) Diversity and spatial distribution of prokaryotic communities along a sediment vertical profile of a deep-sea mud volcano. Microb Ecol 62:655–668CrossRefGoogle Scholar
  15. Pachiadaki MG, Kormas KA (2013) Interconnectivity versus isolation of prokaryotic communities in European deep-sea mud volcanoes. Biogeosciences 10:2821–2831CrossRefGoogle Scholar
  16. Pachiadaki MG, Lykousis V, Stefanou EG, Kormas KA (2010) Prokaryotic community structure and diversity in the sediments of an active submarine mud volcano (Kazan mud volcano, East Mediterranean Sea). FEMS Microbiol Ecol 72:429–444CrossRefGoogle Scholar
  17. Pop Ristova P, Wenzhofer F, Ramette A, Felden J, Boetius A (2015) Spatial scales of bacterial community diversity at cold seeps (eastern Mediterranean Sea). ISME J 9:1306–1318CrossRefGoogle Scholar
  18. Rinke C, Schwientek P, Sczyrba A, Ivanova NN et al (2013) Insights into the phylogeny and coding potential of microbial dark matter. Nature 499:431–437CrossRefGoogle Scholar
  19. Rubin-Blum M, Antler G, Turchyn AV, Tsadok R et al (2014) Hydrocarbon-related microbial processes in the deep sediments of the eastern Mediterranean Levantine Basin. FEMS Microbiol Ecol 87:780–796CrossRefGoogle Scholar
  20. Salazar G, Cornejo-Castillo FM, Benitez-Barrios V, Fraile-Nuez E et al (2016) Global diversity and biogeography of deep-sea pelagic prokaryotes. ISME J 10:596–608CrossRefGoogle Scholar
  21. Scheller S, Yu H, Chadwick GL, McGlynn SE, Orphan VJ (2016) Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction. Science 351:703–707CrossRefGoogle Scholar
  22. Solden L, Lloyd K, Wrighton K (2016) The bright side of microbial dark matter: Lessons learned from the uncultivated majority. Curr Opin Microbiol 31:217–226CrossRefGoogle Scholar
  23. Stach EM, Bull A (2005) Estimating and comparing the diversity of marine Actinobacteria. Antonie Van Leeuwenhoek 87:3–9CrossRefGoogle Scholar
  24. Vanreusel A, Andersen AC, Boetius A, Connelly D et al (2009) Biodiversity of cold seep ecosystems along the European margins. Oceanography 22:110–127CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.University of ThessalyVolosGreece

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