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Induced Pluripotent Stem Cells

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Essential Current Concepts in Stem Cell Biology

Part of the book series: Learning Materials in Biosciences ((LMB))

Abstract

Reprogramming of somatic cells from various species into induced pluripotent stem (iPS) cells has been possible using ectopic expression of pluripotency-associated transcription factors like Oct4, Sox2 and Nanog. iPS cells can model genetic diseases when generated from patient-derived cells and emerge as a source for autologous and allogenic cell therapeutic applications when combined with genome editing e.g. CRISPR/Cas9 nuclease systems. In this chapter, you will be introduced to pluripotency and reprogramming, induction of pluripotent stem cells and their characterization, strategies to allow genome editing of iPS cells and their applications.

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Acknowledgments

Our work was supported by financial contributions and grants from the German Ministry of Research and Education (BMBF), the German State of North Rhine Westphalia (NRW), the Max Planck Society (MPG) and Ruhr University Bochum (RUB), Medical Faculty.

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Authors and Affiliations

  1. Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr-University Bochum, Bochum, Germany

    Holm Zaehres

  2. Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany

    Holm Zaehres

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  1. Holm Zaehres
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Correspondence to Holm Zaehres .

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  1. Department of Anatomy & Molecular Embryology, Ruhr-University Bochum, Bochum, Nordrhein-Westfalen, Germany

    Beate Brand-Saberi

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Zaehres, H. (2020). Induced Pluripotent Stem Cells. In: Brand-Saberi, B. (eds) Essential Current Concepts in Stem Cell Biology. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-33923-4_7

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