Controlling the Optical Properties of Biological Materials

  • Luís Manuel Couto Oliveira
  • Valery Victorovich Tuchin
Part of the SpringerBriefs in Physics book series (SpringerBriefs in Physics)


To overcome the high light-scattering problem that occurs in biological tissues, we present in this chapter the different clearing methods known today. Most of these methods have benefits and downsides, depending on the application for which they are used. The optical immersion method is introduced as a better, reliable, and reversible way to turn tissues clear. The major benefits and advantages of this method such as its reversibility, the lack of side effects, and application in large wavelength range will be presented. A description of the molecular diffusion of optical clearing agents is given to explain the reduction in the refractive index mismatch that natural tissues have.


Control of optical properties Temperature control Mechanical control Tissue optical clearing Immersion optical clearing Scattering reduction Refractive index matching 


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Copyright information

© The Author(s), under exclusive license to Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Luís Manuel Couto Oliveira
    • 1
  • Valery Victorovich Tuchin
    • 2
    • 3
    • 4
    • 5
  1. 1.Physics Department and Center for Innovation in Engineering and Industrial TechnologyPolytechnic Institute of Porto – School of EngineeringPortoPortugal
  2. 2.Department of Optics and BiophotonicsSaratov State UniversitySaratovRussia
  3. 3.Institute of Precision Mechanics and Control of the RASSaratovRussia
  4. 4.Bach Institute of BiochemistryResearch Center of Biotechnology of the RASMoscowRussia
  5. 5.Tomsk State University, Tomsk & ITMO UniversitySt. PetersburgRussia

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