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Reference Materials in Measurement and Technology

RMMT 2018: Reference Materials in Measurement and Technology pp 57–65Cite as

Reference Materials for Ensuring the Metrological Traceability of Enzyme Catalytic Activity Measurement (KATAL)

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Abstract

The article considers problems associated with the metrological support of catalytic activity measurement. The paper presents the characteristics of the State Primary Special Measurement Standard for the unit of catalytic activity—KATAL—and examines the role of reference materials (RMs) in ensuring the metrological traceability of measurement results. A method for measuring the concentration of the recombinant protective antigen of Bacillus anthracis and the recombinant GP-protein of the Ebola virus, involving the use of a “sandwich”-type enzyme-linked immunosorbent assay, was certified by VNIIMS as a measurement procedure. The following RMs tested by VNIIMS can be used for the development and production of appropriate control samples when performing measurements for comparisons: the RM for the mass concentration of the recombinant protective antigen of Bacillus anthracis in phosphate-saline solution, the RM for the mass concentration of the recombinant GP-protein of the Ebola virus and the RM for the mass concentration of recombinant Clostridium Difficile toxin. The paper presents the main reactions of the method for measuring the catalytic activity of catalysts for heterogeneous processes, which are also used to carry out reaction gas cleaning processes. Thus, conditions have been created for building hierarchies of calibrations of in-demand objects established on the basis of the List of Critical Technologies of the Russian Federation and state programs for the development of industrial sectors.

Keywords

  • Metrological traceability
  • State primary measurement standard KATAL
  • Catalytic activity
  • Catalytic concentration
  • Laboratory medicine
  • Reference materials of biological media
  • Heterogeneous solid-state catalysts
  • Calibration hierarchies

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Fig. 1

Abbreviations

ELISA:

Enzyme-linked immunosorbent assay

IRM:

Flow of the initial reaction mixture

FRM:

Flow of the final (contact) reaction mixture

CFR:

Circulating flow reactor

References

  1. Federal Law (2008) On ensuring the uniformity of measurements. No FZ-102 of 26/06/2008. Available at http://www.fundmetrology.ru/depository/01_npa/102-fz_2015.pdf (In Russ)

  2. International Vocabulary of Metrology (2010) Basic and general concepts and associated terms. Translation from English and French is made. D. I. Mendeleyev Institute for Metrology, Belarusian State Institute of Metrology, 82 p (In Russ)

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  3. The final report on the implementation of scientific research work. Conducting fundamental research in the field of measurements of the physicochemical composition and properties of substances with the aim of creating a State Primary Special Measurement Standard for the unit of catalytic activity of biological and chemical substances—KATAL. VNIIMS 1:4 (2016)

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Author information

Authors and Affiliations

  1. All-Russian Research Institute of Metrological Service (VNIIMS), Moscow, Russia

    Elena V. Kulyabina, Olga N. Melkova & Ekaterina A. Guskova

  2. N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation (NITSEM Named After N. F. Gamaleya of the Ministry of Health of the Russian Federation), Moscow, Russia

    Tatiana V. Grebennikova

Authors
  1. Elena V. Kulyabina
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  2. Olga N. Melkova
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  3. Ekaterina A. Guskova
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  4. Tatiana V. Grebennikova
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Corresponding authors

Correspondence to Elena V. Kulyabina or Tatiana V. Grebennikova .

Editor information

Editors and Affiliations

  1. Ural Scientific Research Institute for Metrology, Ekaterinburg, Russia

    Dr. Sergei V. Medvedevskikh

  2. Ural Scientific Research Institute for Metrology, Ekaterinburg, Russia

    Olga N. Kremleva

  3. A.P. Vinogradov Institute of Geochemistry Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    Dr. Irina E. Vasil’eva

  4. Ural Scientific Research Institute for Metrology, Ekaterinburg, Russia

    Dr. Egor P. Sobina

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Kulyabina, E.V., Melkova, O.N., Guskova, E.A., Grebennikova, T.V. (2020). Reference Materials for Ensuring the Metrological Traceability of Enzyme Catalytic Activity Measurement (KATAL). In: Medvedevskikh, S., Kremleva, O., Vasil’eva, I., Sobina, E. (eds) Reference Materials in Measurement and Technology. RMMT 2018. Springer, Cham. https://doi.org/10.1007/978-3-030-32534-3_6

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