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Metrological Assurance of Dioxin Measurements. A Certified Reference Material for Measuring the Dioxin Mass Fraction in Animal Fat

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This article presents information on the development of a new type of certified reference material (CRM) of the mass fraction of dioxins in animal fat, assured with metrological traceability. A procedure for preparing and certifying the CRM is provided. GC-LRMS in combination with isotopic dilution was used as an analytical method for establishing certified characteristics. The uncertainties induced by the CRM inhomogeneity and instability were estimated in order to account for their contribution to the combined uncertainty of the obtained certified values. In addition, the results of interlaboratory comparisons (ILC) studies for an experimental batch of the developed CRM are presented. Measurement results provided by ILC participants are shown to be in good agreement with the certified characteristics of the CRM. The certified values of the mass fractions of dioxins (PCDF group) in the CRM are established to vary from 1 to 1000 ng/kg, with the relative expanded uncertainty ranging from 10 to 20%.


  • Certified reference materials
  • Dioxin
  • Certified value
  • Uncertainty
  • Traceability
  • Mass balance method
  • Inhomogeneity
  • Stability

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  • DOI: 10.1007/978-3-030-32534-3_2
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The authors express their sincere gratitude to the staff of the Scientific Production Association “Typhoon”, Scientific-Technical Centre of Radiation and Chemical Safety and Hygiene of the Federal Medical Biological Agency of Russia (STC RCSH), A. N. Severtsov Institute of Ecology and Evolution, Kuban State Agrarian University and Federal Office of Consumer Protection and Food Safety (BVL), Germany, for their participation in interlaboratory comparisons.

Author Contribution Statement

Budko A. G.: Preparation of the reference material, collection and analysis of experimental data, literature review, preparation of the manuscript draft;

Mikheeva A. Y.: Literature review, revision of the manuscript;

Krylov A. I.: Critical analysis and revision of the manuscript.

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Correspondence to Alexandra G. Budko .

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Budko, A.G., Mikheeva, A.Y., Krylov, A.I. (2020). Metrological Assurance of Dioxin Measurements. A Certified Reference Material for Measuring the Dioxin Mass Fraction in Animal Fat. In: Medvedevskikh, S., Kremleva, O., Vasil’eva, I., Sobina, E. (eds) Reference Materials in Measurement and Technology. RMMT 2018. Springer, Cham.

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