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Point-of-Care Testing System of Uric Acid for the Prevention from Urolithiasis Recurrence

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Future Trends in Biomedical and Health Informatics and Cybersecurity in Medical Devices (ICBHI 2019)

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Abstract

In this study, a simple, novel and inexpensive third generation electrochemical uric acid (UA) biosensor based on three-electrode point-of-care test strip was proposed. On the low-cost screen-printed three silver-based electrodes test strip, the Ag/AgCl reference electrode was first formed by simple electrodeposition with one silver electrode, then the working electrode was prepared by the successive coating of redox mediator, polymer hydrogel, and uricase onto the surface of another silver electrode, the third bare silver electrode was served as the counter electrode to complete the uric acid test strip, which has the advantages of lower oxidation potential, faster response time, higher sensitivity, and wider detecting range.

The three-electrode uric acid test strip specifically and directly senses uric acid in the test sample, and the signal is promptly transferred to the amperometric readout circuit that a stable bias voltage is applied by a bandgap circuit. After initial processing, the analog signal is converted to a digital signal which is calculated with the algorithm of microcontroller to produce a value of user-readable mode. Finally, the digital result is displayed on the liquid-crystal-display (LCD) panel. The complete set of uric acid point-of-care testing system will be used to assess the uric acid condition in the urine of a urolithiasis patient and will be helpful in the diagnosis of urolithiasis.

This uric acid biosensor can be used for testing uric acid either in urine or blood specimen. As combined with other biosensors such as calcium ions, pH, and conductivity, etc., it can be extended to develop a multi-parameter detection system and apply for the prevention of urolithiasis recurrence.

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References

  1. Silverio, A.A.: A multi-sensor readout interface circuit with system-on-chip implementation applied to urine quality analysis (Doctoral dissertation). Chung Yuan Christian University, Taoyuan, Taiwan (2016)

    Google Scholar 

  2. Kao, C.-Y.: Amperometric uric acid biosensor and two-dimensional liquid chromatography-mass spectrometer for urinary uric acid assay (Master’s thesis). Chung Yuan Christian University, Taoyuan, Taiwan (2016)

    Google Scholar 

  3. Healthy.io at. https://healthy.io/

  4. Yue, Y., Guojun, W., Jing, Z., Jianjun, L., Jian, Z., Junwu, Z.: Progresses of preparation and applications of paper-based surface-enhanced raman scattering substrate. Acta Agronomica Sinica 55(3), 0307011 (2018). https://doi.org/10.3788/cjl201845.0307011

    Article  Google Scholar 

  5. Chauhan, N., Pundir, C.S.: An amperometric uric acid biosensor based on multiwalled carbon nanotube–gold nanoparticle composite. Anal. Biochem. 2011(413), 97–103 (2011)

    Article  Google Scholar 

  6. Tsai, C.-M.: The improved study of miniature IrO2/Ta2O5-base potentiometric sensor to carbon dioxide (Master’s thesis). National Chiao Tung University, Hsinchu, Taiwan (2009)

    Google Scholar 

  7. Chauhan, N., Preeti, P., Pundir, C.S.: covalent immobilization of uricase inside a plastic vial for uric acid determination in serum and urine. Anal. Sci. 30, 501–506 (2014)

    Article  Google Scholar 

  8. Bhawna, Pundir, C.S.: Fabrication of dissolved O_2 metric uric acid biosensor based on uricase bound to PVC membrane. J. Sci. Ind. Res. 69, 695–699 (2010)

    Google Scholar 

  9. Li, C.X., Zeng, Y.L., Tang, C.R.: Glucose biosensor based on Carbon/PVC-COOH/Ferrocene composite with covalently immobilized enzyme. Chin. Chem. Lett. 16(10), 1357–1360 (2005)

    Google Scholar 

  10. Chang, S.-Y.: Multi-parameter system-on-a-chip (SoC) design for the prevention of urolithiasis recurrence (Master’s thesis). Chung Yuan Christian University, Taoyuan, Taiwan (2016)

    Google Scholar 

  11. Chang, S-Y.: Multi-parameter system-on-a-chip (SoC) design for the prevention of urolithiasis recurrence (Master’s thesis). Chung Yuan Christian University, Taoyuan, Taiwan (2016)

    Google Scholar 

  12. Cheng, S.-C.: A novel mixed-mode signal processor design by current-mode circuits for biomedical sensing system (Master’s thesis). Chung Yuan Christian University, Taoyuan, Taiwan (2009)

    Google Scholar 

  13. Chang, S.-K.: Compound circuit for the prevention of urolithiasis recurrence (Master’s thesis). Chung Yuan Christian University, Taoyuan, Taiwan (2017)

    Google Scholar 

  14. Kuo, C.-N.: Microelectrode arrays for biosensor application (Master’s thesis). National Chung Hsing University, Taichung, Taiwan (2014)

    Google Scholar 

  15. Chao, C.-T.: Fabrication and test of an amperometric uric acid biosensor (Master’s thesis). National Taiwan University of Science and Technology, Taipei, Taiwan (2009)

    Google Scholar 

  16. Martin, S.M., Gebara, F.H., Strong, T.D., Brown, R.B.: A low-voltage, chemical sensor interface for systems-on-chip: the fully-differential potentiostat. IEEE Cat. No. 04CH37512 (2004). https://doi.org/10.1109/iscas.2004.1329148

  17. Chu, C.-H.: System design and implementation for amperometric electrochemical sensor (Master’s thesis). Chung Yuan Christian University, Taoyuan, Taiwan (2011)

    Google Scholar 

  18. Lin, L-S.: Selective determination of uric acid in the presence of ascorbic acid at screen-printed carbon electrode modified with electrochemically pretreated carbon nanotube. National Sun Yat-sen University, Kaohsiung, Taiwan (2010)

    Google Scholar 

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Acknowledgment

The authors would like to acknowledge Bioptik Technology Inc. Taiwan for the technical support.

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Correspondence to Lin-Chen Yen .

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Yen, LC., Cheng, C., Chung, WY., Tsai, V. (2020). Point-of-Care Testing System of Uric Acid for the Prevention from Urolithiasis Recurrence. In: Lin, KP., Magjarevic, R., de Carvalho, P. (eds) Future Trends in Biomedical and Health Informatics and Cybersecurity in Medical Devices. ICBHI 2019. IFMBE Proceedings, vol 74. Springer, Cham. https://doi.org/10.1007/978-3-030-30636-6_9

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  • DOI: https://doi.org/10.1007/978-3-030-30636-6_9

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  • Publisher Name: Springer, Cham

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  • Online ISBN: 978-3-030-30636-6

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