Abstract
Spectral-domain optical coherence tomography (SD-OCT) produces high-resolution images of retinal cross sections and is becoming a method of choice for in vivo analyses of retinal morphology in rodents. We have adopted this technology to identify and analyze alterations of retinal structure, particularly those with regional and subtle changes. In this technical brief, we will demonstrate the use of SD-OCT in identifying subtle changes in retinal structure and morphology due to the effect of mosaic gene deletion in conditional knockout mice and of uneven distribution of intravitreally delivered compounds, review the application of SD-OCT in measuring pathological lesion volumes, and discuss the major benefits of SD-OCT technology over the traditional histological methods.
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References
Berger A, Cavallero S, Dominguez E, Barbe P, Simonutti M, Sahel JA, Sennlaub F, Raoul W, Paques M, Bemelmans AP (2014) Spectral-domain optical coherence tomography of the rodent eye: highlighting layers of the outer retina using signal averaging and comparison with histology. PLoS One 9:e96494
Dong S, Liu Y, Zhu M, Xu X, Le YZ (2014) Simplified system to investigate alteration of retinal neurons in diabetes. Adv Exp Med Biol 801:139–143
Fu S, Zhu M, Wang C, Le YZ (2014) Efficient induction of productive Cre-mediated recombination in retinal pigment epithelium. Mol Vis 20:480–487
Fu S, Dong S, Zhu M, Sherry DM, Wang C, You Z, Haigh JJ, Le YZ (2015) Müller glia are a major cellular source of survival signals for retinal neurons in diabetes. Diabetes 64:3554–3563
Hee MR, Izatt JA, Swanson EA, Huang D, Schuman JS, Lin CP, Puliafito CA, Fujimoto JG (1995) Optical coherence tomography of the human retina. Arch Ophthalmol 113:325–332
Huang D, Swanson EA, Lin CP, Schuman JS, Stinson WG, Chang W, Hee MR, Flotte T, Gregory K, Puliafito CA et al (1991) Optical coherence tomography. Science 254:1178–1181
Ikeda HO, Sasaoka N, Koike M, Nakano N, Muraoka Y, Toda Y, Fuchigami T, Shudo T, Iwata A, Hori S, Yoshimura N, Kakizuka A (2014) Novel VCP modulators mitigate major pathologies of rd10, a mouse model of retinitis pigmentosa. Sci Rep 4:5970
Le YZ (2011) Conditional gene targeting: dissecting the cellular mechanisms of retinal degenerations. J Ophthalmol 2011:806783
Le YZ, Zheng W, Rao PC, Zheng L, Anderson RE, Esumi N, Zack DJ, Zhu M (2008) Inducible expression of cre recombinase in the retinal pigmented epithelium. Invest Ophthalmol Vis Sci 49:1248–1253
Qiu F, Matlock G, Chen Q, Zhou K, Du Y, Wang X, Ma JX (2017) Therapeutic effects of PPARalpha agonist on ocular neovascularization in models recapitulating neovascular age-related macular degeneration. Invest Ophthalmol Vis Sci 58:5065–5075
Ryals RC, Andrews MD, Datta S, Coyner AS, Fischer CM, Wen Y, Pennesi ME, McGill TJ (2017) Long-term characterization of retinal degeneration in Royal College of surgeons rats using spectral-domain optical coherence tomography. Invest Ophthalmol Vis Sci 58:1378–1386
Zheng L, Anderson RE, Agbaga MP, Rucker EB 3rd, Le YZ (2006) Loss of BCL-XL in rod photoreceptors: increased susceptibility to bright light stress. Invest Ophthalmol Vis Sci 47:5583–5589
Acknowledgments
We declare no conflicts of interest. Our work was supported by NIH grants R01EY20900, R01EY26970, P30EY021725, and P30GM122744, grants from International Retinal Research Foundation, Research to Prevent Blindness, Presbyterian Health Foundation, Oklahoma Center for the Advancement of Science and Technology, and Oklahoma Center for Adult Stem Cell Research and endowments from Mr. Harold Hamm and Choctaw Nation.
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Qiu, F., Zhu, M., Le, YZ. (2019). Noninvasive Diagnosis of Regional Alteration of Retinal Morphology and Structure with Optical Coherence Tomography in Rodents. In: Bowes Rickman, C., Grimm, C., Anderson, R., Ash, J., LaVail, M., Hollyfield, J. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 1185. Springer, Cham. https://doi.org/10.1007/978-3-030-27378-1_25
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DOI: https://doi.org/10.1007/978-3-030-27378-1_25
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