Discussion and Summary

Abstract

FLIO adds a number of benefits to traditional fundus autofluorescence imaging. There is emerging evidence that with FLIO hidden details of the visual cycle can be exposed. Thus, FLIO potentially provides information about the viability of photoreceptors in retinal diseases. In the last years, reports on fluorescence lifetime imaging of macular pigment, retinal dystrophies, and several vascular and degenerative diseases have been published and have brought new insights into the use of FLIO for retinal diagnostics. Important steps have been made to establish acquisition protocols and standards for data analysis and interpretation.