Abstract
Myolaminar Layer Arrangement plays an essential role in cardiac biomechanics. In this preliminary study, we investigate the local 3D arrangement of the myocytes inside the sheets (layers) in three LV human heart transparietal samples imaged by X-ray phase contrast micro-tomography. We extract the large cleavage planes (CPs) of the extracellular matrix, manually select the middle wall region within each sample and compute the skeleton surface (chamfer distance and nonwitness-points selection) of the layers containing the myocytes. We compute the transverse angles of the myocytes in windows (32 × 32 × 32 voxels i.e. 112 × 112 × 112 μm3) centered on the 3D skeleton surface. Our results show that the myocytes are organized (i) in two populations in a LV samples close to the base with an angular distribution alternatively changing from one layer to the next and (ii) in a continuous angular evolution in samples located close to the apex. We find a mean angular difference between the two populations of about 8° in the two LV posterior samples and about 13° in the LV anterior sample. It is too early to statistically confirm that values as “universal” therefore we currently pursue our analysis of other available human LV samples to assess those first results.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Weber, K.T.: Cardiac interstitium in health and disease: the fibrillar collagen network. J. Am. Coll. Cardiol. 13, 1637–1652 (1989)
LeGrice, I.J., Takayama, Y., Covell, J.W.: Transverse shear along myocardial cleavage planes provides a mechanism for normal systolic wall thickening. Circ. Res. 77, 182–193 (1995)
Costa, K.D., Takayama, Y., McCulloch, A.D., Covell, J.W.: Laminar fiber architecture and three-dimensional systolic mechanics in canine ventricular myocardium. Am. J. Physiol. 276, H595–H607 (1999)
Spotnitz, H.M., Spotnitz, W.D., Cottrell, T.S., Spiro, D., Sonnenblick, E.H.: Cellular basis for volume related wall thickness changes in the rat left ventricle. J. Mol. Cell Cardiol. 6, 317–331 (1974)
Takayama, Y., Costa, K.D., Covell, J.W.: Contribution of laminar myofiber architecture to load-dependent changes in mechanics of LV myocardium. Am. J. Physiol. Circ. Physiol. 51, H1510 (2002)
Harrington, K.B., et al.: Direct measurement of transmural laminar architecture in the anterolateral wall of the ovine left ventricle: new implications for wall thickening mechanics. Am. J. Physiol. Circ. Physiol. 288, H1324–H1330 (2005)
Jouk, P.S., et al.: Analysis of the fiber architecture of the heart by quantitative polarized light microscopy. Accuracy, limitations and contribution to the study of the fiber architecture of the ventricles during fetal and neonatal life. Eur. J. Cardio-Thoracic Surg. 31, 916–922 (2007)
LeGrice, I.J., Smaill, B.H., Chai, L.Z., Edgar, S.G., Gavin, J.B., Hunter, P.J.: Laminar structure of the heart: ventricular myocyte arrangement and connective tissue architecture in the dog. Am. J. Physiol. Circ. Physiol. 269, H571–H582 (1995)
Rademakers, F.E., et al.: Relation of regional cross-fiber shortening to wall thickening in the intact heart. Three-dimensional strain analysis by NMR tagging. Circulation 89, 1174–1182 (1994)
Mirea, I., Wang, L., Varray, F., Zhu, Y.-M., Serrano, E.E.D., Magnin, I.E.: Statistical analysis of transmural laminar microarchitecture of the human left ventricle. In: 2016 IEEE 13th International Conference on Signal Processing (ICSP), pp. 53–56. IEEE (2016)
Kung, G.L., et al.: The presence of two local myocardial sheet populations confirmed by diffusion tensor MRI and histological validation. J. Magn. Reson. Imaging 34, 1080–1091 (2011)
Gilbert, S.H., et al.: Visualization and quantification of whole rat heart laminar structure using high-spatial resolution contrast enhanced MRI. Am. J. Physiol. Circ. Physiol. 302, H287–H298 (2011)
Ubbink, S., Bovendeerd, P., Delhaas, T., Arts, T., van de Vosse, F.: Left ventricular shear strain in model and experiment: the role of myofiber orientation. In: Frangi, A.F., Radeva, P.I., Santos, A., Hernandez, M. (eds.) FIMH 2005. LNCS, vol. 3504, pp. 314–324. Springer, Heidelberg (2005). https://doi.org/10.1007/11494621_32
Streeter Jr., D.D., Bassett, D.L.: An engineering analysis of myocardial fiber orientation in pig’s left ventricle in systole. Anat. Rec. 155(4), 503–511 (1966)
Streeter Jr., D.D.: Gross morphology and fiber geometry of the heart. In: Berne, R.M. (ed.) Handbook of Physiology-The Cardiovascular System I. The Heart, vol. 1, chap. 4, pp. 61–112. American Physiology Society, Bethesda (1979)
Bovendeerd, P.H.M., Huyghe, J.M., Arts, T., Van Campen, D.H., Reneman, R.S.: Influence of endocardial-epicardial crossover of muscle fibers on left ventricular wall mechanics. J. Biomech. 27(7), 941–951 (1994)
Varray, F., Mirea, I., Langer, M., Peyrin, F., Fanton, L., Magnin, I.E.: Extraction of the 3D local orientation of myocytes in human cardiac tissue using X-ray phase-contrast micro-tomography and multi-scale analysis. Med. Image Anal. 38, 117–132 (2017)
Otsu, N.: A threshold selection method from gray-level histograms. IEEE Trans. Syst. Man Cybern. 9, 62–66 (1979)
Borgefors, G.: Distance transformations in arbitrary dimensions. Comput. Vis. Graph Image Process 27, 321–345 (1984)
Pudney, C.: Distance-ordered homotopic thinning: a skeletonization algorithm for 3D digital images. Comput. Vis. Image Underst. 72(404–413), 0680 (1998)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this paper
Cite this paper
Wang, S., Mirea, I., Varray, F., Liu, WY., Magnin, I.E. (2019). Investigating the 3D Local Myocytes Arrangement in the Human LV Mid-Wall with the Transverse Angle. In: Coudière, Y., Ozenne, V., Vigmond, E., Zemzemi, N. (eds) Functional Imaging and Modeling of the Heart. FIMH 2019. Lecture Notes in Computer Science(), vol 11504. Springer, Cham. https://doi.org/10.1007/978-3-030-21949-9_23
Download citation
DOI: https://doi.org/10.1007/978-3-030-21949-9_23
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-21948-2
Online ISBN: 978-3-030-21949-9
eBook Packages: Computer ScienceComputer Science (R0)