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The Terpene Synthase Gene Family in Norway Spruce

  • Xue-Mei Yan
  • Shan-Shan Zhou
  • Ilga M. Porth
  • Jian-Feng MaoEmail author
Chapter
  • 134 Downloads
Part of the Compendium of Plant Genomes book series (CPG)

Abstract

Terpenes (isoprenes) represent a diverse group of chemical compounds that plants produce during normal development (e.g., phytohormones), and mostly as secondary metabolites with important roles in defense responses against diverse environmental stressors, either abiotic or biotic in nature. Studies with an aspect on conifer-derived terpenes have highlighted some of the underlying metabolic and molecular mechanisms in the implicated defense processes. Terpene synthases (TPSs), being the core enzymes for terpenes’ functional diversity, therefore, gained attention as the key elements for molecular terpene studies. Norway spruce (Picea abies) is arguably Europe’s native conifer with the most substantial economic and ecological value given its vast geographic distribution throughout the continent as an indigenous species and, introduced, as an important plantation species outside its natural range. In this study, we aimed at retrieving TPS genes from the genomes of Norway spruce and, in addition, of representative land plant lineages in order to resolve their phylogenetic relationship. We show that the majority of TPS genes from gymnosperms are distributed within the TPS-d subfamily. As expected, the “DDXXD” and the “RXR” motifs are highly conserved for TPS in general, and the structural characteristics of closely related TPS genes are highly similar. Concrete subfamily membership along with shared structural molecular properties was the main driver of gene expression variation among Norway spruce TPSs, indicating important functional divergence. In this study, the two key factors within TPS gene structure that were related to differential TPS gene expression were found to be motif composition and intron size. Our study is valuable for further in-depth functional evaluation of these additionally uncovered spruce TPSs and will support future efforts in metabolic engineering involving terpenes.

Keywords

Picea abies Terpene synthase Gene structure Conserved motif Gene expression Functional divergence 

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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Xue-Mei Yan
    • 1
  • Shan-Shan Zhou
    • 1
  • Ilga M. Porth
    • 2
    • 3
    • 4
  • Jian-Feng Mao
    • 1
    Email author
  1. 1.National Engineering Laboratory for Tree Breeding, School of Nature Conservation, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingChina
  2. 2.Département des sciences du bois et de la forêtUniversité LavalQuebec CityCanada
  3. 3.Institute for System and Integrated Biology, Pavillon Charles-Eugène-MarchandUniversité LavalQuebec CityCanada
  4. 4.Centre d’Étude de la ForêtUniversité LavalQuebec CityCanada

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