Abstract
Offering many fundamental and practical advantages of relevance for today’s chemical and biological industry, microreactors are rapidly replacing round-bottomed flasks in modern biochemical labs. Compared to the batch processing, microreactors can offer precise control of reaction variables, high-throughput scanning of reaction conditions, increased safety parameters and they require small quantities of reagents. These miniaturized devices are widely used to facilitate routine work in biochemical analysis and also have applications in biocatalysis where they are termed as enzymatic microreactors. In this work, enzymatic microreactors are used to ease characterization of CaLB enzyme activity as a function of a substrate concentration. The work demonstrates potential of Lab-on-a-Chip devices with enzymatic microreactors as an evolving platforms for performing reliable on-chip biocatalyctic measurements and a possible foundation for future flow-chemistry based industrialization.
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References
Calabrese, G.S., Pissavini, S.: From batch to continuous flow processing in chemicals manufacturing. AIChE J. (2011). https://doi.org/10.1002/aic.12598
Watts, P., Wiles, C.: Recent advances in synthetic micro reaction technology. Chem. Commun. (2007). https://doi.org/10.1039/B609428G
Bisswanger, H.: Enzyme Kinetics. Wiley-Blackwell (2005). https://doi.org/10.1002/3527601759.ch2
Bogdan, A., Poe, S., Kubis, D., Broadwater, S., McQuade, D.: The continuous-flow of ibuprofen. Angew. Chem. Int. Ed. (2009). https://doi.org/10.1002/anie.200903055
Balogh-Weiser, D., Nmeth, C., Ender, F., Gyarmati, B., Szilgyi, A., Poppe, L.: Electrospun nanofibers for entrapment of biomolecules. IntechOpen (2018). https://doi.org/10.5772/intechopen.76068
Bartsch, S., Bornscheuer, U.: A single residue influences the reaction mechanism of ammonia lyases and mutases. Angew. Chem. Int. Ed. (2009). https://doi.org/10.1002/anie.200900337
Fletcher, P.D.I., Haswell, S.J., Pombo Villar, E., Warrington, B., Watts, P., Wong, S.Y., Zhang, X.: Micro reactors: principles and applications in organic synthesis (2002). https://doi.org/10.1016/S0040-4020(02)00432-5
Weiler, A., Junkers, M.: Using microreactors in chemical synthesis: Batch process versus continuous. Flow. Sigma Aldrich ChemFIles
Urban, P.L., Goodall, D.M., Bruce, N.C.: Enzymatic microreactors in chemical analysis and kinetic studies. Biotechnol. Adv. https://doi.org/10.1016/j.biotechadv.2005.06.001
Datta, S., Christena, L.R., Rajaram, Y.R.S.: Enzyme immobilization: an overview on techniques and support materials (2013). https://doi.org/10.1007/s13205-012-0071-7
Strnia, F., Baji, M., Panjan, P., Plazl, I., Sesay, A.M., Žnidar Plazl, P.: Characterization of an enzymatic packed-bed microreactor: Experiments and modeling. Chem. Eng. J. (2018). https://doi.org/10.1016/j.cej.2018.05.028
Sharma, N.: Calb lipase. Fermenta Biotech Limited (2011). https://www.fermentabiotech.com/calb-lipase.php
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Hamidović, M., Ender, F., Springer, A. (2020). A Novel Enzymatic Microreactor: Towards Transforming the Pharmaceutical Industry. In: Badnjevic, A., Škrbić, R., Gurbeta Pokvić, L. (eds) CMBEBIH 2019. CMBEBIH 2019. IFMBE Proceedings, vol 73. Springer, Cham. https://doi.org/10.1007/978-3-030-17971-7_46
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DOI: https://doi.org/10.1007/978-3-030-17971-7_46
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