Abstract
Studies of arthropod microbiota and arthropod-microbe interactions are helping elucidate the strategies adopted by arthropods to colonize and succeed in complex environments, as well as leading to the development of unique pest management approaches. Tetranychoidea (Acariformes: Trombidiformes) are important pests of several crops due to their feeding habits and transmission of pathogens. In tetranychoid mites, the endosymbiont bacterium Cardinium represents a tantalizing first target for pest management research because it affects mite reproduction. In this study, we used previously published 16S ribosomal RNA sequence data of the microbiome bacteria in Brevipalpus yothersi (Baker 1949), Raoiella indica Hirst, 1924, and Oligonychus sp. and the PICRUSt pipeline to predict the content of genes with metabolic function in the bacteriome of the mites. Our results indicate that the bacteriomes of B. yothersi and Oligonychus sp. (which harbor Cardinium) contain significantly more genes involved in the metabolism of indole-alkaloids, glutamine, and biotin when compared with R. indica (which has no Cardinium). The genes for metabolism of biotin and nicotinate are also more abundant in adult B. yothersi and Oligonychus sp. than in their eggs, which is associated with lower abundance of Cardinium in the eggs. The metabolic specialization of Cardinium-dominated bacteriomes could also lead to lack of resistance to β-lactam antibiotics and DDT. While these results are predictive, they highlight the necessity of testing these variations in laboratory. We also present initial data on fungal microbial diversity associated with four different strains of the phytophagous mite vector Brevipalpus, which showed significant variation between strains, while all are dominated by the skin- and surface-specialist genus Malassezia.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Aksoy S (2000) Tsetse—a haven for microorganisms. Parasitol Today 16:114–118
Angleró-Rodríguez YI, Blumberg BJ, Dong Y, Sandiford SL, Pike A, Clayton AM et al (2016) A natural Anopheles-associated Penicillium chrysogenum enhances mosquito susceptibility to Plasmodium infection. Sci Rep 6:34084. https://doi.org/10.1038/srep34084
Boekhout T, Theelen B, Houbraken J, Robert V, Scorzetti G, Gafni A, Gerson U, Sztejnberg A (2003) Novel anamorphic mite-associated fungi belonging to the Ustilaginomycetes: Meirageulakonigii gen. nov., sp. nov., Meira argovae sp. nov. and Acaromyces ingoldii gen. nov., sp. nov. Int J Syst Evol Microbiol 53:1655–1664
Busby PE, Soman C, Wagner MR, Friesen ML, Kremer J, Bennett A, Morsy M, Eisen JA, Leach JE, Dangl JL (2017) Research priorities for harnessing plant microbiomes in sustainable agriculture. PLoS One 15(3):e2001793. https://doi.org/10.1371/journal.pbio.2001793
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010a) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336
Caporaso JG, Bittinger K, Bushman FD, Desantis TZ, Andersen GL, Knight R (2010b) PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26:266–267
Crotti E, Balloi A, Hamdi C, Sansonno L, Marzorati M, Gonella E, Favia G, Cherif A, Bandi C, Alma et al (2012) Microbial symbionts: a resource for the management of insect-related problems. Microb Biotechnol 5:307–317
Douglas AE (2007) Symbiotic microorganisms: untapped resources for insect pest control. Trends Biotechnol 25:338–342
Duarte AP, Ferro M, Rodrigues A, Bacci M Jr, Nagamoto NS, Forti LC, Pagnocca FC (2016) Prevalence of the genus Cladosporium on the integument of leaf-cutting ants characterized by 454 pyrosequencing. Antonie Van Leeuwenhoek 109(9):1235–1243
Feldhaar H, Straka J, Krischke M, Berthold K, Stoll S, Mueller MJ, Gross R (2007) Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia. BMC Biol 5:48
Hawksworth DL (2012) Global species numbers of fungi: are tropical studies and molecular approaches contributing to a more robust estimate? Biodivers Conserv 21:2425–2433
Hart BJ, Douglas AE (1991) The relationship between house-dust mites and fungi. In: The Acari. Springer, Dordrecht
Ihrmark K, Bödeker, ITM, Cruz-Martinez K, Friberg H, Kubartova A, Schenck J, Strid Y, Stenlid J, Brandström-Durling M, Clemmensen KE, Lindahl BD (2012) New primers to amplify the fungal ITS2 region— evaluation by 454-sequencing of artificial and natural communities. FEMS Microbiol Ecol 82(3):666–677. https://doi.org/10.1111/j.1574-6941.2012.01437.x
Iturbe-Ormaetxe I, Walker T, O’Neill SL (2011) Wolbachia and the biological control of mosquito-borne disease. EMBO Rep 12(6):508–518. http://doi.org/10.1038/embor.2011.84
Kitajima EW, Rezende JAM, Rodrigues JCV (2003) Passion fruit green spot virus vectored by Brevipalpus phoenicis (Acari: Tenuipalpidae) on passion fruit in Brazil. Exp Appl Acarol 30:225–231
Langille MGI, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes J, Clemente JC, Burkepile DE, Vega Thurber RL et al (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31:814–821
Lustgraaf B (1978) Ecological relationships between xerophilic fungi and house-dust mites (Acarida: Pyroglyphidae). Oecologia 33:351–359. https://doi.org/10.1007/BF00348118
Malacrinò A, Schena L, Campolo O, Laudani F, Mosca S, Giunti G, Strano CP, Palmeri V (2017) A metabarcoding survey on the fungal microbiota associated to the olive fruit fly. Microb Ecol 73(3):677–684
Maoz Y, Gal S, Argov Y, Coll M, Palevsky E (2011) Biocontrol of persea mite, Oligonychus perseae, with an exotic spider mite predator and an indigenous pollen feeder. Biol Control 59:147–157
Nakabachi A, Ishikawa H (1999) Provision of riboflavin to the host aphid, Acyrthosiphon pisum, by endosymbiotic bacteria, Buchnera. J Insect Physiol 45:1–6
Ospina O, Massey SE, Rodrigues JCV (2016) Reduced diversity in the bacteriome of the phytophagous mite Brevipalpus yothersi (Acari: Tenuipalpidae). Insects 7:80
Parks DH, Tyson GW, Hugenholtz P, Beiko RG (2014) STAMP: statistical analysis of taxonomic and functional profiles. Bioinformatics 30:3123–3124
Quan AS, Eisen MB (2018) The ecology of the Drosophila-yeast mutualism in wineries. PLoS One 13(5):e0196440
Rodrigues JCV (2000) Relações patógeno-vetor-planta no sistema leprose dos citros. PhD dissertation, São Paulo University Piracicaba, Brazil
Rodrigues JCV, Childers CC (2013) Brevipalpus mites (Acari: Tenuipalpidae): vectors of invasive, non-systemic cytoplasmic and nuclear viruses in plants. Exp Appl Acarol 59:165–175
Rodrigues JCV, Gallo-Meagher M, Ochoa R, Childers CC, Adams BJ (2004) Mitochondrial DNA and RAPD polymorphisms in the haploid mite Brevipalpus phoenicis (Acari: Tenuipalpidae). Exp Appl Acarol 34:275–290
Rodrigues JCV, Ochoa R, Kane EC (2007) First report of Raoiella indica Hirst (Acari: Tenuipalpidae) and its damage to coconut palms in Puerto Rico and Culebra Island. Int J Acarol 33:3–5
Rodrigues JCV, Childers CC, Kitajima EW (2016) Brevipalpus spp. (Acari: Tenuipalpidae): vectors of cytoplasmic and nuclear viruses in plants. In: Brown JK (ed) Vector-mediated transmission of plant pathogens. American Phytopathological Society, St. Paul, MN, pp 309–318
Sabree ZL, Kambhampati S, Moran NA (2009) Nitrogen recycling and nutritional provisioning by Blattabacterium, the cockroach endosymbiont. Proc Natl Acad Sci U S A 106:19521–19526
Saldaña MA, Hegde S, Hughes GL (2017) Microbial control of arthropod-borne disease. Memórias do Instituto Oswaldo Cruz 112(2):81–93
Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA 109:6241–6246
Smith TA, Driscoll T, Gillespie JJ, Raghavan R (2015) A Coxiella-like endosymbiont is a potential vitamin source for the lone star tick. Genome Biol Evol 7:831–838
Tanaka E, Shimizu K, Imanishi Y, Yasuda Y, Tanaka C (2008) Isolation of basidiomycetous anamorphic yeast-like fungus Meira argovae found on Japanese bamboo. Mycoscience 49(5):329–333
The Human Microbiome Project (HMP) Consortium (2012) Structure, function and diversity of the healthy human microbiome. Nature 486:207–214
Wang JJ, Dong P, Xiao LS, Dou W (2008) Effects of removal of Cardinium infection on fitness of the stored-product pest Liposcelis bostrychophila (Psocoptera: Liposcelididae). J Econ Entomol 101:1711–1717
Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73:5261–5267
Weeks AR, Marec F, Breeuwer JAJ (2001) A mite species that consists entirely of haploid females. Science 292:2479–2482
Weeks AR, Velten R, Stouthamer R (2003) Incidence of a new sex-ratio-distorting endosymbiotic bacterium among arthropods. Proc R Soc B Biol Sci 270:1857–1865
Zalar P, Sybren de Hoog G, Schroers HJ, Frank JM, Gunde-Cimerman N (2005) Antonie Van Leeuwenhoek 87:311–328. https://doi.org/10.1007/s10482-004-6783-x
Zchori-Fein E, Perlman SJ (2004) Distribution of the bacterial symbiont Cardinium in arthropods. Mol Ecol 13:2009–2016
Acknowledgements
To USDA/APHIS 8130-0059-CA and USDA-Hatch 427 for partial financing of this work and NIFA-06242 grant for infrastructural improvement.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Rodrigues, J.C.V., Ospina, O.E., Massey, S.E. (2019). Mycobiome of Brevipalpus Mite Strains and Insights on Metabolic Function in the Bacteriome of the Tetranychoidea Mites. In: Skvarla, M., Ochoa, R., Verle Rodrigues, J., Hutcheson, H. (eds) Contemporary Acarology . Springer, Cham. https://doi.org/10.1007/978-3-030-17265-7_5
Download citation
DOI: https://doi.org/10.1007/978-3-030-17265-7_5
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-17264-0
Online ISBN: 978-3-030-17265-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)