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Intraluminal Middle Cerebral Artery Occlusion Model in Mice

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Animal Models of Acute Neurological Injury

Part of the book series: Springer Series in Translational Stroke Research ((SSTSR))

Abstract

Intraluminal middle cerebral artery occlusion (MCAO) model in rodents has been widely used for cerebral ischemia studies. The procedure is relatively simple and non-invasive compared to the other focal cerebral ischemia models, and the ischemic insult is highly reproducible. The use of genetically modified mice has been attracting attention because it allows us to dissect molecular and cellular mechanisms of various pathological conditions in the brain and therefore, the mouse intraluminal MCAO model has become more prevalent. The authors describe here our method of mouse intraluminal MCAO and characterized its effects on infarct areas, local cerebral blood flow, extracellular amino acids concentration and physiological parameters.

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Authors and Affiliations

  1. Division of Neurosurgery, Department of Neuro and Locomotor Science, Akita University School of Medicine, Akita, Japan

    Masaya Oda, Taku Sugawara, Kohei Kokubun & Kazuo Mizoi

  2. Department of Neurosurgery, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi, Japan

    Hiroyuki Kinouchi

Authors
  1. Masaya Oda
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  2. Taku Sugawara
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  3. Kohei Kokubun
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  4. Kazuo Mizoi
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  5. Hiroyuki Kinouchi
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Corresponding author

Correspondence to Hiroyuki Kinouchi .

Editor information

Editors and Affiliations

  1. Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Jun Chen

  2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA

    Zao C. Xu

  3. Indiana University School of Medicine, SNRI SCBIRG, Indianapolis, IN, USA

    Xiao Ming Xu

  4. Center for Neuroscience Research, Loma Linda University School of Medicine, Loma Linda, CA, USA

    John H. Zhang

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Oda, M., Sugawara, T., Kokubun, K., Mizoi, K., Kinouchi, H. (2019). Intraluminal Middle Cerebral Artery Occlusion Model in Mice. In: Chen, J., Xu, Z., Xu, X., Zhang, J. (eds) Animal Models of Acute Neurological Injury. Springer Series in Translational Stroke Research. Springer, Cham. https://doi.org/10.1007/978-3-030-16082-1_11

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  • DOI: https://doi.org/10.1007/978-3-030-16082-1_11

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-16080-7

  • Online ISBN: 978-3-030-16082-1

  • eBook Packages: MedicineMedicine (R0)

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