Abstract
Proteomics-based mass spectrometry has gained increasing amounts of popularity in recent years. In particular, high resolution accurate mass measurements in mass spectrometry has gained notoriety for giving the capability of high throughput analysis with lower cost to the user. In particular, its uses in the identification of protein sequence through the utilization of bottom-up, middle-down, and top-down approaches has been widely discussed. In this chapter, we discuss the advantages of each technique as well as using the techniques in tandem to gain well-rounded structural data on our protein of interest, glucokinase. The study will focus on the use of Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry, but give insights into the advantages that may come from the utilization of other high resolution techniques.
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Acknowledgements
We would like to thank Dr. Craig Dufresne (ThermoFisher) for his support and expertise in bringing this project to light. We gratefully acknowledge the financial support of the National Institutes of Health through the National Center for Research Resources (Grant #S10-RR029517-01) for providing funding used to obtain the instrumentation used in the research and the University at Buffalo for financial support for this research.
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Sekera, E.R., Wood, T.D. (2019). Sequencing Proteins from Bottom to Top: Combining Techniques for Full Sequence Analysis of Glucokinase. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 1140. Springer, Cham. https://doi.org/10.1007/978-3-030-15950-4_6
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DOI: https://doi.org/10.1007/978-3-030-15950-4_6
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