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Trends in Analysis of Cortisol and Its Derivatives

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Book cover Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1140))

Abstract

Determination of concentration of cortisol in various biological fluids can provide extensive information about a person’s health. Historically, cortisol and its derivatives were (and still are) determined using immunoaffinity-based methods such as colorimetric ELISA assay, chemiluminescent immunoassay, fluorescence assays, radioimmunoassay, electrochemiluminescence immunoassay, immunochromatographic test, or sensors and immunosensors. Recently, mass spectrometry (MS)-based methods started to be used in determination of cortisol and its derivatives. These MS methods are net superior to immunoaffinity-based assays, but are not easily applicable and are also time-consuming and price prohibitive. Furthermore the standard MS instruments used are triple quadrupole instruments. Here we review the literature on the MS and non-MS based methods for determination of cortisol and its derivatives and also explore the use of a less used quadrupole-time of flight instrument in determination of these compounds.

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Acknowledgements

We thank Isagenix Corporation (grant #ISAG-375-100409) for funding that allowed us to determine the concentrations of caffeine and cortisol in saliva samples.

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  1. Ali Boolani and Devika Channaveerappa contributed equally to this work.

Authors and Affiliations

  1. Applied Physiology and Psychology Lab, Department of Physical Therapy, Clarkson University, Potsdam, NY, USA

    Ali Boolani & Stephanie Grobe

  2. Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Devika Channaveerappa, Emmalyn J. Dupree, Madhuri Jayathirtha, Roshanak Aslebagh, Tyler Wilkinson & Costel C. Darie

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  1. Ali Boolani
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Correspondence to Costel C. Darie .

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  1. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Alisa G. Woods

  2. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Costel C. Darie

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Boolani, A. et al. (2019). Trends in Analysis of Cortisol and Its Derivatives. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 1140. Springer, Cham. https://doi.org/10.1007/978-3-030-15950-4_39

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