Investigation of Antibody-Drug Conjugates by Mass Spectrometry

  • Madhuri Jayathirtha
  • Costel C. DarieEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1140)


ADCs are empowered monoclonal antibodies that are designed to harness their targeting ability by linking them to cell-killing agents. They are made up of three main components, the antibody, linker and the cytotoxic drug. The specificity of the antibody with the antigen on the tumor cell surface helps with its internalization into the cell after which the active drug is released causing cell death. The investigation of ADCs can be done using a variety of MS methods. Here, we talk about the bottom-up approach, the top-down approaches such as ECD and ETD, the ESI/MS method and IM-MS. Further, we also focus on the applications of MALDI/MS such as UV-MALDI, IR-MALDI and IMS-MALDI and provide examples of the mass spectra that provide tremendous amount of information on ADC structures.


Mass spectrometry Antibody-drug conjugates Protein characterization Therapeutics 



Antibody-drug conjugates


Anaplastic large cell lymphoma


Complementary determining regions


Drug to antibody ratio


Electron capture dissociation


Electrospray ionization


Electron transfer dissociation


Hodgkin’s lymphoma


Ion mobility


Monoclonal antibodies


Matrix assisted laser desorption ionization


Monomethyl auristatin E


Mass spectrometry


Posttranslational modification


Small cell lung carcinoma


THIOMAB drug conjugates


Time of flight



We would like to thank the past and current lab members for creating a pleasant working environment.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular ScienceClarkson UniversityPotsdamUSA

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