In this paper, a new preprocessing algorithm to qualify images of different pollen grains for further processing is proposed. This algorithm provides a score related to the sharpness of the image and will be used to automatically adjust the focal length of a microscope that magnifies the image. The obtained score has been compared to four quality metrics generally used to estimate the clarity of an image and to a reference made by a human. The results of the simulations show that the proposed algorithm combines better performance with low complexity on the set of images.
- Microscope slide image acquisition
- Sharp image detection
- Fourier transform