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Current Advancements in Recombinant Technology for Industrial Cellulases: Part-I

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Approaches to Enhance Industrial Production of Fungal Cellulases

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Cellulases are one of the widely used enzymes in different industries such as pharma, paper, detergent, agriculture, and food. These are gaining significant interest nowadays owing to their ability to convert the cellulosic biomass in to valuable products including second-generation biofuels and other bioprocess-based products. There has been a significant advancement in the commercial production of cellulase enzyme by using recombinant DNA technology. Recombinant DNA technology facilitates the conversion of noncellulolytic-producing microbes to enzyme-producing one by transferring the desired genes from known microbes. The gene responsible for cellulase production can be extracted from the cellulolytic microbes and can be inserted and expressed in target bacterium using a suitable vector. The gene of interest can be transferred by direct or indirect gene transfer method in the fast replicating microbe. Fungi and molds are predominant sources of cellulase enzyme, but their production and recovery cost is higher which makes them unsuitable for commercial production. The potential recombinant microbe producing cellulase can enhance the overall product yield by showing enormous growth potential and production on inexpensive substrate such as agro-industrial waste in lesser time making the process economical. This chapter deals with the new advancement in cellulase production by recombinant strains. The chapter comprises the information regarding different gene construct expressing cellulase enzyme, their fermentative production, recovery, and potential applications at industrial level.

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Authors and Affiliations

  1. Centre of Food Science and Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India

    Abhishek Dutt Tripathi, Kamlesh Kumar Maurya, Sadhna Mishra & Diksha Shaw

  2. School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, India

    Suresh Kumar Srivastava

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  1. Abhishek Dutt Tripathi
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  2. Suresh Kumar Srivastava
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  3. Kamlesh Kumar Maurya
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  4. Sadhna Mishra
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  5. Diksha Shaw
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Editors and Affiliations

  1. Department of Physics and Astrophysics, University of Delhi, Delhi, Delhi, India

    Manish Srivastava

  2. Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, Varanasi, Uttar Pradesh, India

    Neha Srivastava

  3. Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, Uttar Pradesh, India

    Pramod W. Ramteke

  4. Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, Varanasi, Uttar Pradesh, India

    Pradeep Kumar Mishra

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Tripathi, A.D., Srivastava, S.K., Maurya, K.K., Mishra, S., Shaw, D. (2019). Current Advancements in Recombinant Technology for Industrial Cellulases: Part-I. In: Srivastava, M., Srivastava, N., Ramteke, P., Mishra, P. (eds) Approaches to Enhance Industrial Production of Fungal Cellulases . Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-14726-6_10

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