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Sample Treatment for Saliva Proteomics

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Emerging Sample Treatments in Proteomics

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1073))

Abstract

The preanalytical phase of saliva proteomics/peptidomics, which includes sample collection, handling, and storage, represents a major challenge for any researcher that envisions sensitive and high-throughput analyses, coupled to well-controlled study design. The methodology used to collect saliva determines the contribution of each salivary gland to saliva composition with impact on data retrieved from proteomics/peptidomics. The awareness of the importance of this step in the analysis of saliva has prompted the proposal of several collection strategies. Moreover, numerous commercial devices are available in an attempt to routine the procedures. However, whatever the chosen method, procedures should be kept simple, standardized to get better reproducibility and repeatability on saliva proteomics analysis. Sample preservation is also a key step in saliva proteomics/peptidomics, and the implemented lab procedures should avoid posttranslational modifications such as proteolysis, as well as protein precipitation.

In this chapter, we provide recommendations for saliva sampling and preservation, envisaging to standardize procedures that facilitate the use of saliva in clinical applications and the translation of proteomics data to diagnosis and/or definition of therapeutic targets.

“Saliva

doesn’t have the drama of blood,

it doesn’t have the integrity of sweat and

it doesn’t have the emotional appeal of tears.”

—Irwin Mandel (professor emeritus, Columbia University)

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Authors and Affiliations

  1. QOPNA/LAQV, Departamento de Química da Universidade de Aveiro, Aveiro, Portugal

    Francisco Amado & Rita Ferreira

  2. CESPU, IINFACTS, Departamento das Ciências Dentárias do IUCS, Gandra, PRD, Portugal

    Maria João Calheiros-Lobo

  3. iBiMED, Department of Medical Sciences, Portugal, University of Aveiro, Aveiro, Portugal

    Rui Vitorino

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  1. BIOSCOPE Group, LAQV-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Proteomass Scientific Society, Rua dos Inventores, Madan Park, Caparica, Portugal

    José-Luis Capelo-Martínez

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Amado, F., Calheiros-Lobo, M.J., Ferreira, R., Vitorino, R. (2019). Sample Treatment for Saliva Proteomics. In: Capelo-Martínez, JL. (eds) Emerging Sample Treatments in Proteomics. Advances in Experimental Medicine and Biology(), vol 1073. Springer, Cham. https://doi.org/10.1007/978-3-030-12298-0_2

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