Abstract
Immunoassays are biochemical tests that utilize antibodies as reagents to detect the presence and/or concentration of ANALYTES. This chapter focuses on immunoassays; however the principles apply also to other ligand binding assays that do not utilize antibodies but instead bind the ANALYTE with ligands, receptors, binding proteins, or other macromolecules. The basic principle of the immunoassay is antibody-antigen interaction, which typically is of high AFFINITY and specificity. This feature allows the detection and quantification of low-abundant ANALYTES. ANALYTES measured by immunoassays can be small molecule drugs, hormones, glycans/saccharides, lipids, peptides, proteins, and even larger structures like viruses and bacteria. Immunoassays measure ANALYTES not only in pure systems (e.g., buffer, cell medium) but also in complex MATRICES including cell lysate, serum, plasma, urine, synovial fluid, bronchoalveolar lavage, sputum, and amniotic fluid. The result can be available in a few minutes (e.g., pregnancy tests); however, it is more typical that a few hours pass between sample preparation and result.
Final manuscript submitted on September 07, 2017.
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References
Yalow RS, Berson SA. Assay of plasma insulin in human subjects by immunological methods. Nature. 1959;184. (Suppl 21:1648–9.
Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975;256(5517):495–7.
Corren J, Lemanske RF, Hanania NA, Korenblat PE, Parsey MV, Arron JR, et al. Lebrikizumab treatment in adults with asthma. N Engl J Med. 2011;365(12):1088–98.
Wild D, editor. The immunoassay handbook. 4th ed. Amsterdam: Elsevier Science; 2013.
Dinis-Oliveira RJ. Heterogeneous and homogeneous immunoassays for drug analysis. Bioanalysis. 2014;6(21):2877–96.
Schwickart M, Vainshtein I, Lee R, Schneider A, Liang M. Interference in immunoassays to support therapeutic antibody development in preclinical and clinical studies. Bioanalysis. 2014;6(14):1939–51.
Ian Weeks LK, Wild D. Signal generation and detection systems (excluding homogenous assays). In: The immunoassay handbook. Amsterdam: Elsevier Science; 2013.
Ullman EF. Homogenous immunoassays, Chapter 2.3. In: The immunoassay handbook. 4th ed. Amsterdam: Elsevier Science; 2013.
Scott RAW. Enhanced chemiluminescent immunoassays. In: The protein protocols handbook. Basel: Springer Nature Switzerland AG; 2009.
Huang X, Aguilar ZP, Xu H, Lai W, Xiong Y. Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: a review. Biosens Bioelectron. 2016;75:166–80.
Chang L, Li J, Wang L. Immuno-PCR: an ultrasensitive immunoassay for biomolecular detection. Anal Chim Acta. 2016;910:12–24.
Riedel T, Majek P, Rodriguez-Emmenegger C, Brynda E. Surface plasmon resonance: advances of label-free approaches in the analysis of biological samples. Bioanalysis. 2014;6(24):3325–36.
Lee JW, Devanarayan V, Barrett YC, Weiner R, Allinson J, Fountain S, et al. Fit-for-purpose method development and validation for successful biomarker measurement. Pharm Res. 2006;23(2):312–28.
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Schwickart, M., Vainshtein, I. (2019). Immunoassays. In: Parnham, M., Nijkamp, F., Rossi, A. (eds) Nijkamp and Parnham's Principles of Immunopharmacology. Springer, Cham. https://doi.org/10.1007/978-3-030-10811-3_15
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DOI: https://doi.org/10.1007/978-3-030-10811-3_15
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