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Convegno Nazionale Sensori

CNS 2018: Sensors pp 309–316Cite as

DNA-Based Biosensor on Flexible Nylon Substrate by Dip-Pen Lithography for Topoisomerase Detection

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Part of the Lecture Notes in Electrical Engineering book series (LNEE,volume 539)

Abstract

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

Keywords

  • Flexible device
  • Molecular printing
  • Biosensor
  • Topoisomerase

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Authors and Affiliations

  1. Department of Chemical Science, University of Catania, v.le A. Doria 6, 95125, Catania, Italy

    V. Ferrara & Giovanni Marletta

  2. Department of Biology, University of Rome Tor Vergata, via della Ricerca Scientifica, 00133, Rome, Italy

    A. Ottaviani & A. Desideri

  3. Dipartimento di Fisica e Chimica, Università di Palermo, v.le delle Scienze, 90128, Palermo, Italy

    F. Cavaleri, G. Arrabito, C. Pellerito & B. Pignataro

  4. Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, v.le delle Scienze, 90128, Palermo, Italy

    P. Cancemi & S. Feo

  5. Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China

    Y.-P. Ho

  6. Department of Molecular Biology and Genetics and INANO, Aarhus University, Møllers Allé 3, Aarhus, Denmark

    B. R. Knudsen & M. S. Hede

Authors
  1. V. Ferrara
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  2. A. Ottaviani
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  3. F. Cavaleri
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  4. G. Arrabito
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  5. P. Cancemi
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  6. Y.-P. Ho
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  7. B. R. Knudsen
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  8. M. S. Hede
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  9. C. Pellerito
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  10. A. Desideri
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  11. S. Feo
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  12. Giovanni Marletta
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  13. B. Pignataro
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Corresponding author

Correspondence to B. Pignataro .

Editor information

Editors and Affiliations

  1. University of Catania, Catania, Italy

    Prof. Bruno Andò

  2. IFAC-CNR, Sesto Fiorentino, Florence, Italy

    Dr. Francesco Baldini

  3. University of Rome Tor Vergata, Rome, Italy

    Prof. Corrado Di Natale

  4. Department of Information Engineering (DII), University of Brescia, Brescia, Italy

    Prof. Vittorio Ferrari

  5. University of Catania, Catania, Italy

    Dr. Vincenzo Marletta

  6. Department of Chemistry, University of Florence, Sesto Fiorentino, Florence, Italy

    Prof. Giovanna Marrazza

  7. University of Palermo, Palermo, Italy

    Prof. Valeria Militello

  8. University of Padova, Padua, Italy

    Prof. Giorgia Miolo

  9. Sapienza University of Rome, Rome, Italy

    Prof. Marco Rossi

  10. Università Politecnica delle Marche (UNIVPM), Ancona, Italy

    Dr. Lorenzo Scalise

  11. IMM-CNR, Lecce, Italy

    Pietro Siciliano

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Ferrara, V. et al. (2019). DNA-Based Biosensor on Flexible Nylon Substrate by Dip-Pen Lithography for Topoisomerase Detection. In: , et al. Sensors. CNS 2018. Lecture Notes in Electrical Engineering, vol 539. Springer, Cham. https://doi.org/10.1007/978-3-030-04324-7_39

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  • DOI: https://doi.org/10.1007/978-3-030-04324-7_39

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-04323-0

  • Online ISBN: 978-3-030-04324-7

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