Abstract
Determining the order of nucleotide in the biological macromolecules has laid the foundation for understanding the biology of an organism. Over the last 50 years, genomics has been revolutionized with the researchers producing newer techniques and technologies leading to innovations in the field of DNA sequencing. This period has witnessed revolutionary developments in terms of progressing from simple oligonucleotide sequencing to massive parallel sequencing of millions of bases, from struggling to map the coding sequences of single gene to whole genome-wide annotations. The use of sequencing has increased tremendously, and an advance in sequencing technologies over the years has made it accessible to research and clinical labs all over the world. With the introduction of massive parallel sequencing technologies, the overload of data onto the scientists has become the biggest challenge. The Human Genome Project took around 13 years to draft the first human genome sequence. But, now with the advancement of the sequencing technology and bioinformatics tools, it could be done in few hours to days. The DNA sequencing techniques are key tools in the scientific world revolutionizing many fields of science and are increasingly used in health care especially in the field of oncology, inherited disorders, and infectious diseases. The current chapter traverses in the chronological order, describing different generations of sequencing technology, underlining few key discoveries, scientists, and sequences along the way.
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References
Ambardar S, Gupta R et al (2016) High throughput sequencing: an overview of sequencing chemistry. Indian J Microbiol 56(4):394–404
Ansorge WJ (2009) Next-generation DNA sequencing techniques. New Biotechnol 25(4):195–203
Ari Ş, Arikan M (2016) Next-generation sequencing: advantages, disadvantages, and future. In: Plant omics: trends and applications. Springer, Berlin, pp 109–135
Branton D, Deamer D, Marziali A, Bayley H, Benner S, Butler T, Di Ventra M, Garaj S, Hibbs A, Huang X, Jovanovich S, Krstic P, Lindsay S, Ling X, Mastrangelo C, Meller A, Oliver J, Pershin Y, Ramsey J, Riehn R, Soni G, Tabard-Cossa V, Wanunu M, Wiggin M, Schloss J (2008) The potential and challenges of nanopore sequencing. Nat Biotechnol 26(10):1146–1153
Bragg L, Tyson GW (2014) Metagenomics using next-generation sequencing. Methods Mol Biol 1096:183–201
Brown SM, Goecks J (2015) RNA sequencing with next-generation sequencing Chapter 13: RNA sequencing with next-generation sequencing. Cold Spring Harbor Laboratory press, Second Edition
Buermans HPJ, den Dunnen JT (2014) Next generation sequencing technology: advances and applications. Biochim Biophys Acta (BBA) - Mol Basis Dis 1842(10):1932–1941
Deamer D, Akeson M, Branton D (2016) Three decades of nanopore sequencing. Nat Biotechnol 34(5):518–524
Der Sarkissian C, Allentoft ME et al (2015) Ancient genomics. Philos Trans R Soc Lond B Biol Sci 370(1660):20130387
Dewey FE, Pan S et al (2012) DNA sequencing: clinical applications of new DNA sequencing technologies. Circulation 125(7):931–944
Feng Y, Zhang Y et al (2015) Nanopore-based fourth-generation DNA sequencing technology. Genomics Proteomics Bioinformatics 13(1):4–16
França LTC, Carrilho E, Kist TBL (2002) A review of DNA sequencing techniques. Q Rev Biophys 35(02)
Gaastra W (1985) Chemical cleavage (Maxam and Gilbert) method for DNA sequence determination. Methods Mol Biol 2:333–341
Golan D, Medvedev P (2013) Using state machines to model the Ion Torrent sequencing process and to improve read error rates. Bioinformatics 29(13):i344–i351
Grada A, Weinbrecht K (2013) Next-generation sequencing: methodology and application. J Invest Dermatol 133(8):e11
Guzvic M (2013) The History of DNA Sequencing. J Med Biochem 32(4):301–312
Harrington CT, Lin EI et al (2013) Fundamentals of pyrosequencing. Arch Pathol Lab Med 137(9):1296–1303
Heather JM, Chain B (2016) The sequence of sequencers: the history of sequencing DNA. Genomics 107(1):1–8
Huang Y, Chen S, Chiang Y, Chen T, Chiu K (2012) Palindromic sequence impedes sequencing-by-ligation mechanism. BMC Systems Biology 6(Suppl 2):S10
Lu H, Giordano F, Ning Z (2016) Oxford Nanopore MinION sequencing and genome assembly. Genomics Proteomics Bioinformatics 14(5):265–279
Masser DR et al (2015) Targeted DNA methylation analysis by next-generation sequencing. J Vis Exp (96):e52488. https://doi.org/10.3791/52488
Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci U S A 74(2):560–564
Men AE, Wilson P, Siemering K, Forrest S (2008) Sanger DNA sequencing. In: Janitz M (ed) Next generation genome sequencing: towards personalized medicine, 1st edn. Wiley-VCH Verlag GmbH & Co, Weinheim
Ravi I, Baunthiyal M, Saxena J (2014) Advances in biotechnology. Springer, New York
Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes JC et al (1977) Nucleotide sequence of bacteriophage |[phi]|X174 DNA. Nature 265(5596):687–695
Slatko BE, Albright LM et al (2001) DNA sequencing by the dideoxy method. Curr Protoc Mol Biol Chapter 7: Unit7 4A
Voelkerding KV, Dames SA, Durtschi JD (2009) Next-generation sequencing: from basic research to diagnostics. Clin Chem 55(4):641–658
Wallis Y, Morrell N (2011) Automated DNA sequencing. Methods Mol Biol 688:173–185
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Shetty, P.J., Amirtharaj, F., Shaik, N.A. (2019). Introduction to Nucleic Acid Sequencing. In: Shaik, N., Hakeem, K., Banaganapalli, B., Elango, R. (eds) Essentials of Bioinformatics, Volume I. Springer, Cham. https://doi.org/10.1007/978-3-030-02634-9_6
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DOI: https://doi.org/10.1007/978-3-030-02634-9_6
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