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Prefoldins: the new chaperones pp 85–94Cite as

Role of the Unconventional Prefoldin Proteins URI and UXT in Transcription Regulation

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1106))

Abstract

The Unconventional prefoldin RPB5 interacting protein (URI), also known as RPB5-Mediating Protein (RMP) has been shown to play several regulatory roles in different cellular compartments including the mitochondria, as a phosphatase binding protein; in the cytoplasm, as a chaperone-like protein; and in the nucleus, as a transcriptional regulator through binding to RPB5 and RNA polymerase II (polII). This chapter focuses on the role URI plays in transcriptional regulation in the prostate cell. In prostate cells, URI is tightly bound to another prefoldin-like protein called UXT, a known androgen receptor (AR) cofactor. Part of a multiprotein complex, URI and UXT act as transcriptional repressors, and URI regulates KAP1 through PP2A phosphatase activity. The discovery of the interaction of URI and UXT with KAP1, AR, and PP2A, as well as the numerous interactions between URI and components of the R2TP/prefoldin-like complex, RPB5, and nuclear proteins involved in DNA damage response, chromatin remodeling and gene transcription, reveal a pleiotropic effect of the URI/UXT complex on nuclear processes. The mechanisms by which URI/UXT affect transcription, chromatin structure and regulation, and genome stability, remain to be elucidated but will be of fundamental importance considering the many processes affected by alterations of URI/UXT and other prefoldins and prefoldin-like proteins.

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Author notes

  1. Authors Phillip A. Thomas and Paolo Mita have equally contributed to this chapter.

Authors and Affiliations

  1. Departments of Urology, and Biochemistry and Molecular Biology, New York University School of Medicine, New York, NY, USA

    Phillip A. Thomas, Susan Ha & Susan K. Logan

  2. Institute for Systems Genetics, New York University School of Medicine, New York, NY, USA

    Paolo Mita

Authors
  1. Phillip A. Thomas
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  2. Paolo Mita
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  3. Susan Ha
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  4. Susan K. Logan
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Corresponding author

Correspondence to Susan K. Logan .

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Editors and Affiliations

  1. Centro Nacional de Investigaciones Oncológicas, CNIO, Madrid, Spain

    Nabil Djouder

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Thomas, P.A., Mita, P., Ha, S., Logan, S.K. (2018). Role of the Unconventional Prefoldin Proteins URI and UXT in Transcription Regulation. In: Djouder, N. (eds) Prefoldins: the new chaperones. Advances in Experimental Medicine and Biology, vol 1106. Springer, Cham. https://doi.org/10.1007/978-3-030-00737-9_6

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