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Metabolome Profiling of Growth Hormone Transgenic Coho Salmon by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

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Abstract

Growth in fish is regulated in part by the growth hormone (GH)-insulin-like growth factor (IGF) axis, and salmon transgenic for GH are known to show dramatic increased growth. However, little is known concerning the in vivo global levels of metabolites and the mechanism of enhancement of growth in GH transgenic vertebrates. The present study examined the charged metabolites levels in GH transgenic coho salmon (Oncorhynchus kisutch) overexpressing GH by metabolomic analysis. Triplicate groups of size-matched (0 year-old, approx. 60 g) and age-matched (1.5 years-old) GH transgenic (T) and non-transgenic (NT) wild salmon were quantitatively assessed for levels of approximately 200 metabolites in both muscle and liver. The most notable difference found between T and NT fish was that glycolysis metabolite levels were increased in the muscle of transgenic fish. In addition, an increase in some metabolite levels in the transgenic fish muscle was found to be enhanced by ration-restriction. However, these effects observed in muscle were different from that seen in liver. The results suggest that GH transgenesis can improve the use of carbohydrates as a source of energy associated with rapid growth. These effects are likely to depend on the level of total digestible energy intake and type of tissue in transgenic fish.

Keywords

Metabolome Growth hormone Transgenic fish CE-TOFMS 

Notes

Acknowledgements

The authors thank staff at Center for Aquaculture and Environment Research, Fisheries and Oceans Canada, Institute for Advanced Biosciences, Keio University, Japan, and Dr. T. Fujimoto at Hokkaido University, Japan for assistance with lab work. TN acknowledges Dr. J. G. Richards, Mr. D. Chung and Miss. H. Bryant at Department of Zoology, The University of British Columbia, Canada, and Drs. M. Sato, T. Yamaguchi, Y. Ochiai, and I. Gleadall at Tohoku University, Japan for valuable discussion. This study was supported in part by KAKENHI grant (#23580277) and a fund to Core-to-Core Program (A. Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” from JSPS to TN and by the Canadian Regulatory System for Biotechnology to RHD.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Marine Biochemistry Laboratory, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  2. 2.Nutrition Laboratory, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  3. 3.Institute of Metabolic ScienceUniversity of CambridgeCambridgeUK
  4. 4.Fisheries and Oceans CanadaWest VancouverCanada
  5. 5.Institute for Advanced BiosciencesKeio UniversityMizukamiJapan

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