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Laboratory diagnosis of ocular toxoplasmosis: serological and PCR based analysis of ocular fluids

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Congenital toxoplasmosis
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Abstract

Inflammation of the uvea is a sight threatening disease affecting people in the prime of their life. Infectious as well as non infectious mechanisms play a role in the pathogenesis of uveitis. Toxoplasmosis, which is caused by the protozoan parasite Toxoplasma gondii, is the most common cause of posterior uveitis in many countries [1,2]. Epidemiological studies have indicated that the incidence of toxoplasmic retinochoroiditis may range from 0.6% in an area in the USA to up to 20% in southern Brazil [3,4].

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References

  1. Holland GN, O’Connor GR, Belfort R Jr, Remington JS (1996) Toxoplasmosis. In: Pepose JS, Holland GN, Wilhelmus KR (eds) Ocular Infection and Immunity-1996. Mosby Year Book Inc St Louis, Missouri

    Google Scholar 

  2. Pavesio CE, Lightman S (1996) Toxoplasma gondii and ocular toxoplasmosis: pathogenesis. Br J Ophthalmol 80:1099–1107

    Article  PubMed  CAS  Google Scholar 

  3. Smith RE, Ganley JP (1972) Ophthalmic survey of a community. 1. Abnormalities of the ocular fundus. Am J Ophthalmol 7:11–26

    Google Scholar 

  4. Glasner PD, Silveira C, Kruszon-Moran D et al (1992) An unusually high prevalence of ocular toxoplasmosis in Southern Brazil. Am J Ophthalmol 114:136

    PubMed  CAS  Google Scholar 

  5. Teutsch SM, Juranek DD, Sulzer A et al (1979) Epidemic toxoplasmosis associated with infected cats. N Engl J Med 300:695–699

    Article  PubMed  CAS  Google Scholar 

  6. Bowie WR, King AS, Werker DH et al (1997) Outbreak of toxoplasmosis associated with municipal drinking water. Lancet 350:173

    Article  PubMed  CAS  Google Scholar 

  7. Loewer-Sieger DH, Rothova A, Koppe JG, Kijlstra A (1984) Congenital toxoplasmosis, a prospective study based on 1821 pregnant women. In: Saari KM, (ed.) Uveitis Update. Amsterdam, Elsevier Science Publishers, Amsterdam

    Google Scholar 

  8. Bosch-Driessen EH, Rothova A (1998) Sense and nonsense of corticosteroid administration in the treatment of ocular toxoplasmosis. Br J Ophthalmol 82:858–860

    Article  PubMed  CAS  Google Scholar 

  9. Rothova A, Van Knapen F, Baarsma GS, Kruit PJ, Loewer-Sieger DH, Kijlstra A (1986) Serology in ocular toxoplasmosis. Br J Ophthalmol 70:615–622

    Article  PubMed  CAS  Google Scholar 

  10. Montoya JG, Remington JS (1996) Toxoplasmic chorioretinitis in the setting of acute acquired toxoplasmosis. Clin Inf Dis 23:277–282

    Article  CAS  Google Scholar 

  11. van der Lelij A, Rothova A (1997) Diagnostic anterior chamber paracentesis in uveitis: a safe procedure? Br J Ophthalmol 81:976–979

    Article  PubMed  Google Scholar 

  12. Kijlstra A, Luyendijk L, Baarsma GS et al (1996) Aqueous humor analysis as a diagnostic tool in Toxoplasma uveitis. Int Ophthalmol 13:383–386

    Article  Google Scholar 

  13. De Boer JH, Verhagen C, Bruinenberg M et al (1996) Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis. Am J Ophthalmol 121:650–658

    PubMed  Google Scholar 

  14. Ronday MJH, Ongkosuwito JV, Rothova A, Kijlstra A (1999) Intraocular anti-Toxoplasma gondii IgA antibody production in patients with ocular toxoplasmosis. Am J Ophthalmol 127:294–300

    Article  PubMed  CAS  Google Scholar 

  15. Klaren VNA, Van Doornik CEM, Ongkosuwito JV, Feron EJ, Kijlstra A (1998) Differences between intraocular and serum antibody responses in patients with ocular toxoplasmosis. Am J Ophthalmol 126:698–706

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

I would like to thank Dr. Jenny Ongkosuwito for critically reading the manuscript.

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© 2000 Springer-Verlag France

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Kijlstra, A. (2000). Laboratory diagnosis of ocular toxoplasmosis: serological and PCR based analysis of ocular fluids. In: Ambroise-Thomas, P., Petersen, P.E. (eds) Congenital toxoplasmosis. Springer, Paris. https://doi.org/10.1007/978-2-8178-0847-5_17

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  • DOI: https://doi.org/10.1007/978-2-8178-0847-5_17

  • Publisher Name: Springer, Paris

  • Print ISBN: 978-2-287-59664-3

  • Online ISBN: 978-2-8178-0847-5

  • eBook Packages: Springer Book Archive

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