Résumé
L’ovocyte qui va être fécondé a grandi en taille au sein de son follicule et s’est entouré de la zone pellucide, structure amorphe acellulaire composée de glycoprotéines au nombre de quatre dans l’espèce humaine (1). Cette zone pellucide va avoir plusieurs fonctions: la reconnaissance et la sélection du spermatozoïde fécondant, ainsi que le déclenchement de sa réaction acrosomique lui permettant de libérer les enzymes qu’il transporte dans l’acrosome. Ceux-ci vont lui permettre de rompre les liaisons entre la ZP1 et les polymères de ZP2-ZP3 afi n de se frayer un passage et de se propulser au travers de la zone pellucide grâce à ses battements flagellaires jusque dans l’espace périvitellin (2).
Preview
Unable to display preview. Download preview PDF.
Références
Lefievre L, Conner SJ, Salpekar A et al. (2004) Four zona pellucida glycoproteins are expressed in the human. Hum Reprod 19:1580–1586
Litscher, E, Williams Z, Wassarman P (2009) Zona pellucida glycoprotein ZP3 and fertilization in mammals. Mol Reprod Dev 76:933–941
De Jonge C (2005) Biological basis for human capacitation. Hum Reprod Update 11:205–214
Yanagimachi R (1978) Sperm-egg association in animals. Curr Top Dev Biol 12:83–105
Longo FJ, Chen DY (1985) Development of cortical polarity in mouse eggs: involvement of the meiotic apparatus. Dev Biol 107:382–394
Wolf JP, Ducot B, Kunstmann JM et al. (1992) Influence of sperm parameters on outcome of subzonal insemination in the case of previous IVF failure. off. Hum Reprod 7:1407–1413
Ziyyat, A, Rubinstein E, Monier-Gavelle F et al. (2006) CD9 controls the formation of clusters that contain tetraspanins and the integrin alpha 6 beta 1, which are involved in human and mouse gamete fusion. J Cell Sci 119:416–424
Chen MS, Tung KS, Coonrod SA et al. (1999) Role of the integrin-associated protein CD9 in binding between sperm ADAM 2 and the egg integrin alpha6beta1: implications for murine fertilization. Proc Natl Acad Sci U S A. 96:11830–11835
Rubinstein E, Ziyyat A, Wolf JP et al. (2006) The molecular players of sperm-egg fusion in mammals. Semin Cell Dev Biol 17:254–263
Primakoff, P, Hyatt H, Tredick-Kline J (1987) Identification and purification of a sperm surface protein with a potential role in sperm-egg membrane fusion. J Cell Biol 104:141–149
Blobel CP, Wolfsberg TG, Turck C et al. (1992) A potential fusion peptide and an integrin ligand domain in a protein active in sperm-egg fusion. Nature 356:248–252
Evans, J. P, Kopf GS, Schultz RM (1997) Characterization of the binding of recombinant mouse sperm fertilin beta subunit to mouse eggs: evidence for adhesive activity via an egg beta1 integrin-mediated interaction. Dev Biol 187:79–93
Takahashi Y, Bigler D, Ito Y, White JM (2001) Sequencespecific interaction between the disintegrin domain of mouse ADAM 3 and murine eggs: role of betal integrin-associated proteins CD9, CD81, and CD98. Mol Biol Cell 12:809–820
Nishimura H, Cho C, Branciforte D et al. (2001) Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta. Dev Biol 233:204–213
Cho C, Ge E, Branciforte D et al. (2000) Analysis of mouse fertilin in wild-type and fertilin beta (-/-) sperm: evidence for C-terminal modification, alpha/beta dimerization, and lack of essential role of fertilin alpha in sperm-egg fusion. Dev Biol 222:289–295
Gould RJ, Polokoff MA, Friedman PA et al. (1990) Disintegrins: a family of integrin inhibitory proteins from viper venoms. Proc Soc Exp Biol Med 195:168–171
Evans JP (2002) The molecular basis of sperm-oocyte membrane interactions during mammalian fertilization. Hum Reprod Update 8:297–311
Tomczuk M, Takahashi Y, Huang J et al. (2003) Role of multiple betal integrins in cell adhesion to the disintegrin domains of ADAMs 2 and 3. Exp Cell Res 290:68–81
Ji YZ, Wolf JP, Jouannet P, Bomsel M (1998) Human gamete fusion can bypass betal integrin requirement. Hum Reprod 13:682–689
Bronson R, Fusi F (1990) Sperm-oolemmal interaction: role of the Arg-Gly-Asp (RGD) adhesion peptide. Fertil Steril. 54:527–529
Fusi FM, Lorenzetti I, Mangili F et al. (1994) Vitronectin is an intrinsic protein of human spermatozoa released during the acrosome reaction. Mol Reprod Dev 39:337–343
Sanchez-Mateos P, Cabanas C, Sanchez-Madrid F (1996) Regulation of integrin function. Semin Cancer Biol 7:99–109
Miyado K, Yamada G., Yamada S, et al. (2000) Requirement of CD9 on the egg plasma membrane for fertilization. Science 287:321–324
Almeida EA, Huovila APJ, Sutherland AE et al. (1995) Mouse egg integrin alpha 6 beta 1 functions as a sperm receptor. Cell 81:1095–1104
He ZY, Brakebusch C, Fassler R et al. (2003) None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion. Dev Biol 254:226–237
Takahashi Y, Yamakawa N, Matsumoto K et al. (2000) Analysis of the role of egg integrins in sperm-egg binding and fusion. Mol Reprod Dev 56:412–423
Barraud-Lange V, Naud-Barriant N, Saffar L et al. (2007) Alpha6beta1 integrin expressed by sperm is determinant in mouse fertilization. BMC Dev Biol 7:10
Fusi FM, Tamburini C, Mangili F et al. (1996) The expression of alpha v, alpha 5, beta 1, and beta 3 integrin chains on ejaculated human spermatozoa varies with their functional state. Mol Hum Reprod 2:169–175
Boissonnas CC, Montjean D, Lesaffre C et al. (2010) Role of sperm alphavbeta3 integrin in mouse fertilization. Dev Dyn 239:773–783
Fusi FM, Bernocchi N, Ferrari A, Bronson R. (1996) Is vitronectin the velcro that binds the gametes together? Mol Hum Reprod 2:859–866
Reddy VR, Rajeev SK, Gupta V (2003) Alpha 6 beta 1 Integrin is a potential clinical marker for evaluating sperm quality in men. Fertil Steril 79Suppl 3:1590–1596
Le Naour, F, Rubinstein E, Jasmin C et al. (2000) Severely reduced female fertility in CD9-deficient mice. Science 287:319–321
Charrin S, Le Naour F, Silvie O et al. (2009) Lateral organization of membrane proteins: tetraspanins spin their web. Biochem J 420:133–154
Hemler ME (2003) Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain. Annu Rev Cell Dev Biol 19:397–422
Zhu GZ, Miller BJ, Boucheix C, et al. (2002) Residues SFQ (173–175) in the large extracellular loop of CD9 are required for gamete fusion. Development 129:1995–2002
Boucheix C, Rubinstein E (2001) Tetraspanins. Cell Mol Life Sci 58:1189–1205
Levy S, Shoham T (2005) The tetraspanin web modulates immune-signalling complexes. Nat Rev Immunol 5:136–148
Charrin S, Le Naour F, Labas V et al. (2003) EWI-2 is a new component of the tetraspanin web in hepatocytes and lymphoid cells. Biochem J 373:409–421
Charrin S, Le Naour F, Oualid M et al. (2001) The major CD9 and CD81 molecular partner. Identification and characterization of the complexes. J Biol Chem 276:14329–14337
Kaji K, Oda S, Miyazaki S, Kudo A (2002) Infertility of CD9-deficient mouse eggs is reversed by mouse CD9, human CD9, or mouse CD81; polyadenylated mRNA injection developed for molecular analysis of sperm-egg fusion. Dev Biol 247:327–334
Inoue N, Ikawa M, Isotani A, Okabe M (2005) The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 434:234–238
Sosnik J, Miranda PV, Spiridonov NA et al. (2009) Tssk6 is required for Izumo relocalization and gamete fusion in the mouse. J Cell Sci 122:2741–2749
Ziyyat A, Naud-Barriant N, Barraud-Lange V et al. (2005) Cyclic FEE peptide increases human gamete fusion and potentiates its RGD-induced inhibition. Hum Reprod 20:3452–3458
Bronson R, Fusi FM, Calzi F et al. (1999) Evidence that a functional fertilin-like ADAM plays a role in human spermoolemmal interactions. Mol Hum Reprod 5:433–440
Barraud-Lange V, Naud-Barriant N, DUCOT B et al. (2008) Cyclic QDE peptide increases fertilization rates and provides healthy pups in mouse. Fertil Steril 9:2110–2115
Joly E, Hudrisier D (2003) What is trogocytosis and what is its purpose? Nat Immunol 4:815
Barraud-Lange V, Naud Barriant N, Bomsel M, et al. (2007) Transfer of oocyte membrane fragments to fertilizing spermatozoa. FASEB J 21:3446–3449
Miyado K, Yoshida K, Yamagata K et al. (2008) The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice. Proc Natl Acad Sci USA 105:12921–12926
Mattioli M, Gloria A, Mauro A et al. (2009) Fusion as the result of sperm-somatic cell interaction. Reproduction 138:679
Phillips DM, Shalgi R (1980) Surface architecture of the mouse and hamster zona pellucida and oocyte. J Ultrastruct Res 72:1–12
Dalo DT, Maccaffery JM, Evans JP (2008) Ultrastuructural analysis of egg menbrane abnormalities in post-ovulatory aged eggs. Int J Dev Biol 52:535–544
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer-Verlag France, Paris
About this chapter
Cite this chapter
Wolf, JP. et al. (2011). L’interaction gamétique au cours de la fécondation. In: Physiologie, pathologie et thérapie de la reproduction chez l’humain. Springer, Paris. https://doi.org/10.1007/978-2-8178-0061-5_4
Download citation
DOI: https://doi.org/10.1007/978-2-8178-0061-5_4
Publisher Name: Springer, Paris
Print ISBN: 978-2-8178-0060-8
Online ISBN: 978-2-8178-0061-5
eBook Packages: MedicineMedicine (R0)