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Comparisons Between Related and Unrelated Cord Blood Collection and/or Banking for Transplantation or Research: The UK NHS Blood and Transplant Experience

  • Suzanne M. Watt
  • Katherine Coldwell
  • Jon Smythe
Chapter

Abstract

Following the pioneering work of Gluckman and colleagues, umbilical cord blood (UCB) has become an important source of hematopoietic stem cells for clinical transplantation, and banked UCB units are now readily available for this purpose. UCB banks now operate in many countries, with more than 10,000 allogeneic transplants having been performed worldwide. Many of the procedures for processing and storage of UCB in use in England today were established at the New York Cord Blood Bank in the 1990s and modified in the National Blood Service (now National Health Service Blood and Transplant or NHSBT). UCB banking within NHSBT was first instigated in 1995 as part of the South East Regional Blood Transfusion Service. The first cord blood units were then banked in February 1996 in dedicated facilities at Edgware in London and, for the following 7 years, altruistic donations were principally obtained through maternity units from two hospitals, Northwick Park and Barnet General NHS Trust hospitals. These hospitals were selected on the basis of their closeness to Edgware, and their high proportion of ethnic minority groups, which were inadequately represented in UK bone marrow donor registries. In 2001, with the nationalization of the blood transfusion services in England and North Wales and prior to the merger of the National Blood Service (NBS) for England and North Wales with UK Transplant to form NHSBT, cord blood collections and banking for clinical transplantation and research were integrated into the Stem Cells and Immunotherapies Department. This Department, now within NHSBT, comprises a network of eight Human Tissue Authority (HTA)-licensed GMP-grade facilities including the NHS Cord Blood Bank (previously the London Cord Blood Bank), specializing in the procurement, testing, processing, banking, and issuing of stem cell harvests or products from umbilical cord blood, mobilized peripheral blood, and bone marrow. The laboratories are also at the forefront for the development and delivery of advanced cellular therapies or related diagnostics using these cell sources. The NHSBT now sources unrelated UCB units from four hospitals in London, collects designated or directed family UCB units throughout England, and also obtains UCB units for research. This chapter will review current UCB collection and banking practices within NHSBT and will summarize the significant changes that have occurred since their inception and integration into the first public cord blood bank for transplantation and research in the UK.

Keywords

Cord Blood Umbilical Cord Blood Total Nucleate Cell Cord Blood Bank Cord Blood Unit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors would like to thank all those staff within the NHSBT specialist services who provided data for this review, and Ms Milly Lymer for her assistance with references.

Grant Funding: The authors wish to acknowledge the support of NHS Blood and Transplant, The Oxford Biomedical Research Centre, The Experimental Cancer Medicine Centre – Oxford, National Institutes of Research UK, Restore Burns & Wound Healing Trust, the Royal College of Surgeons, the Cord Blood Charity, the British Heart Foundation, the Leukaemia Research Fund, and E.U. Framework VI Thercord and VII Cascade Programme Grants.

References

  1. 1.
    Gluckman E, Broxmeyer HE, Auerbacm AD, et al. Haematopoietic reconstitution in a patient with Fanconi’s anaemia by means of umbilical cord from an HLA-identical sibling. N Engl J Med. 1989;321:1174-1178.CrossRefPubMedGoogle Scholar
  2. 2.
    Watt SM, Contreras M. Stem cell medicine: umbilical cord blood and its stem cell potential. Sem Fetal Neonat Med. 2005;28:1-12.Google Scholar
  3. 3.
    Ballen KK. New trends in umbilical cord blood transplantation. Blood. 2005;105:3786-3792.CrossRefPubMedGoogle Scholar
  4. 4.
    Rubinstein P. Why cord blood? Hum Immunol. 2006;67:398-404.CrossRefPubMedGoogle Scholar
  5. 5.
    Brunstein CG, Wagner J. Umbilical cord blood transplantation and banking. Ann Rev Med. 2006;57:403-417.CrossRefPubMedGoogle Scholar
  6. 6.
    Brown JA, Boussiotis VA. Umbilical cord blood transplantation: basic biology and clinical challenges to immune reconstitution. Clin Immunol. 2008;127(3):286-297.CrossRefPubMedGoogle Scholar
  7. 7.
    Pinto FO, Roberts I. Cord blood stem cell transplantation for hematoglobinopathies. Br J Haematol. 2008;141(3):309-324.PubMedGoogle Scholar
  8. 8.
    Tse WW, Zang SL, Bunting KD, Laughlin MJ. Umbilical cord blood transplantation in adult myeloid leukemia. Bone Marrow Transplant. 2008;41(5):465-472.CrossRefPubMedGoogle Scholar
  9. 9.
    Sullivan MJ. Banking on cord blood stem cells. Nat Rev Cancer. 2008;8(7):555-563.CrossRefPubMedGoogle Scholar
  10. 10.
    Tse W, Bunting KD, Laughlin MJ. New insights into cord blood stem cell transplantation. Curr Opin Hematol. 2008;15(4):279-284.CrossRefPubMedGoogle Scholar
  11. 11.
    Smythe J, Armitage S, McDonald D, et al. Directed sibling cord blood banking for transplantation: the 10-year experience in the national blood service in England. Stem Cells. 2007;25(8):2087-2093.CrossRefPubMedGoogle Scholar
  12. 12.
    Meyer EA, Hanna K, Gebbie K. Cord Blood: Establishing a National Hematopoietic Stem Cell Bank Program. Washington, DC: The National Academies Press; 2005.Google Scholar
  13. 13.
    Rocha V, Wagner JE, Sobocinski K, et al. Comparison of graft versus host disease in children transplanted with HLA identical sibling umbilical cord blood versus HLA identical sibling bone marrow transplant. N Engl J Med. 2000;342:1846-1854.CrossRefPubMedGoogle Scholar
  14. 14.
    Broxmeyer HE. Cord Blood: Biology, Immunology, Banking and Clinical Transplantation. Bethesda, MD: AABB Press; 2004.Google Scholar
  15. 15.
    Schoemans H, Theunissen K, Maertens J, et al. Adult umbilical cord blood transplantation: a comprehensive review. Bone Marrow Transplant. 2006;38:83-93.CrossRefPubMedGoogle Scholar
  16. 16.
    Fernandez MN, Regidor C, Cabrera R, et al. Unrelated umbilical cord blood transplants in adults: early recovery of neutrophils by supportive co-transplantation of a low number of highly purified peripheral blood CD34+ cells from an HLA-haploidentical donor. Exp Hematol. 2003;31:535-544.CrossRefPubMedGoogle Scholar
  17. 17.
    Magro E, Regidor C, Cabrera R, et al. Early hematopoietic recovery after single unit unrelated cord blood transplantation in adults supported by co-infusion of mobilized stem cells from a third party. Haematologica. 2006;91:640-648.PubMedGoogle Scholar
  18. 18.
    www.NETCORD.org – Virtual Office.
  19. 19.
    Reed W, Smith R, Dekovic F, et al. Comprehensive banking of sibling donor cord blood for children with malignant and nonmalignant disease. Blood. 2003;101:351-357.CrossRefPubMedGoogle Scholar
  20. 20.
    Walters MC, Quirolo L, Trachtenberg ET, et al. Sibling donor cord blood transplantation for thalassemia major: experience of the sibling donor cord blood program. Ann NY Acad Sci. 2005;1054:206-213.CrossRefPubMedGoogle Scholar
  21. 21.
    Cohen Y, Nagler A. Umbilical cord blood transplantation – how, when and for whom? Blood Rev. 2004;18:167-179.CrossRefPubMedGoogle Scholar
  22. 22.
    Dyer C. Couple is given go-ahead to use embryo selection to help existing child. BMJ. 2006;332:1114.CrossRefPubMedGoogle Scholar
  23. 23.
    Dyer C. HFEA widens its criteria for preimplantation genetic diagnosis. BMJ. 2006;332:1174.CrossRefPubMedGoogle Scholar
  24. 24.
    Liao C, Li D, Wei J, Tang X, Li Y, Huang Y. Prenatal HLA-typing in beta-thalassemia before the collection of sibling cord blood. Prenat Diagn. 2006;26:89-90.CrossRefPubMedGoogle Scholar
  25. 25.
    Qureshi N, Foote D, Walters MC, Singer ST, Quirolo K, Vichinsky EP. Outcomes of preimplantation genetic diagnosis therapy in treatment of {beta}-thalassemia: a retrospective analysis. Ann NY Acad Sci. 2005;1054:500-503.CrossRefPubMedGoogle Scholar
  26. 26.
    Human Tissue Authority Codes of Practice. 2006. Department of Health. London, UK.Google Scholar
  27. 27.
    EU Tissues and Cells Directive (2004/23/E). 2004. Official Journal of the European Union.Google Scholar
  28. 28.
    Austin EB, Guttridge M, Pamphilon D, Watt SM. The role of blood services and regulatory bodies in stem cell transplantation. Vox Sang. 2008;94(1):6-17.PubMedGoogle Scholar
  29. 29.
    Watt SM, Austin E, Armitage S. Cryopreservation of hematopoietic stem/progenitor cells for therapeutic use. Methods Mol Biol. 2007;368:237-259.CrossRefPubMedGoogle Scholar
  30. 30.
    Davey S, Armitage S, Vanderson R, et al. The London cord blood bank; analysis of banking and transplantation outcome. Br J Haematol. 2004;125:358-365.CrossRefGoogle Scholar
  31. 31.
    Warwick R, Armitage S. Cord blood banking. Best Pract Res Clin Obs Gyn. 2004;18:995-1011.CrossRefGoogle Scholar
  32. 32.
    Ashford P, Distler P, Gee A, et al. Terminology and labeling of cellular products: 1. Standards. Bone Marrow Transplant. 2007;40(11):1075-1083.CrossRefPubMedGoogle Scholar
  33. 33.
    Ashford P, Distler P, Gee A, et al. Terminology and labeling of cellular products-2: implementation plan. Bone Marrow Transplant. 2007;40(11):1085-1090.CrossRefPubMedGoogle Scholar
  34. 34.
    Takahashi TA, Rebulla P, Armitage S, et al. Multi-laboratory evaluation of procedures for reducing the volume of cord blood: influence on cell recoveries. Cytotherapy. 2006;8(3):254-264.CrossRefPubMedGoogle Scholar
  35. 35.
    Brown C, Ellis J, Davey S, Brown J, Green A, Smythe J, Ord J, Regan F, Watt S, Navarrete CV. HLA profile and TNC and CD34 content of the NHS Cord Blood Collected and Issued Units. Abstract S135. Trans Med. 2008;18 (suppl. 2): 22.Google Scholar
  36. 36.
    Guidance for the Blood Transfusion Service in the United Kingdom. 7th ed. Norwich, UK: The UK Government Stationary Office; 2005.Google Scholar
  37. 37.
    International Standards for Cord Blood Collection, Processing, Testing, Banking, Selection and Release. 3rd ed. Omaha, NE: Foundation for the Accreditation of Hematopoietic Cell Therapy; 2006.Google Scholar
  38. 38.
    Bunce M, O’Neill CM, Barnardo MC, et al. Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP). Tissue Antigens. 1995;46:355-367.CrossRefPubMedGoogle Scholar
  39. 39.
    Forsi L, Brown J, Railton D, et al. HLA typing of chorionic villus samples: assessment of potential for cord blood collection for the treatment of siblings. Eur J Immunogenetics. 2002;29:363-368.Google Scholar
  40. 40.
    Verlinsky Y, Rechitsky S, Sharapova T, Morris R, Taranissi M, Kuliev A. Preimplantation HLA testing. JAMA. 2004;291:2079.CrossRefPubMedGoogle Scholar
  41. 41.
    The Human Fertilisation and Embryology Authority Code of Practice. 6th ed. London, UK: Department of Health; 2003.Google Scholar
  42. 42.
    Allan DS, Keeney M, Howson-Jan K, et al. Number of viable CD34+ cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation. Bone Marrow Transplant. 2002;29:967-972.CrossRefPubMedGoogle Scholar
  43. 43.
    Rules and Guidance for Pharmaceutical Manufacturers and Distributors. Annex 1- Manufacture of Sterile Medicinal Products. Norwich, UK: The UK Department of Health Stationary Office; 2002.Google Scholar
  44. 44.
    Patel R, Davey S, Turner D, Brown C. The use of STR analysis to confirm identity of a cord blood unit prior to transplantation (abstract). Eur J Immunogenet. 2003;30:310.Google Scholar
  45. 45.
    Nissen-Druey C, Tichelli A, Meyer-Monard S. Human haematopoietic colonies in health and disease. Acta Haematologica. 2005;113:1-96.CrossRefGoogle Scholar
  46. 46.
    Fisk NM, Roberts IA, Markwald R, Mironov V. Can routine commercial cord blood banking be scientifically and ethically justified? PLoS Med. 2005;2:87-90.CrossRefGoogle Scholar
  47. 47.
    Royal College of Obstetricians and Gynaecologists. Umbilical cord blood banking. Scientific advisory committee opinion paper 2. Revised June 2006.Google Scholar
  48. 48.
    Cord Blood Banking for Potential Future Transplantation. Policy Statement. Pediatrics. 2007;119:165-170.CrossRefGoogle Scholar
  49. 49.
    Welte K, Costeas P, Brunoehler D et al. The world marrow donor association (WMDA) policy statement for the utility of autologous or family cord blood unit storage. Approved May 2006.Google Scholar
  50. 50.
    Wagner JE, Barker JN, DeFor TE, et al. Transplantation of unrelated donor umbilical cord blood in 102 patients with malignant and nonmalignant diseases: influence of CD34 cell dose and HLA disparity on treatment-related mortality and survival. Blood. 2002;100:1611-1618.PubMedGoogle Scholar
  51. 51.
    Hamza NS, Lisgaris M, Yadavalli G, et al. Kinetics of myeloid and lymphocyte recovery and infectious complications after unrelated umbilical cord blood versus HLA-matched unrelated donor allogeneic transplantation in adults. Br J Haematol. 2004;124:488-498.CrossRefGoogle Scholar
  52. 52.
    Montagna D, Locatelli F, Moretta A, et al. T lymphocytes of recipient origin may contribute to the recovery of specific immune response toward viruses and fungi in children undergoing cord blood transplantation. Blood. 2004;103:4322-4329.CrossRefPubMedGoogle Scholar
  53. 53.
    Wall DA, Noffsinger JM, Mueckl KA, et al. Feasibility of an obstetrician-based cord blood collection network for unrelated donor umbilical cord blood banking. J Maternal-Fetal Med. 1997;6:320-323.Google Scholar
  54. 54.
    Surbek DV, Visca E, Steinmann C, et al. Umbilical cord blood collection before placental delivery during cesarean delivery increases cord blood volume and nucleated cell number available for transplantation. Am J Obstet Gynecol. 2000;183:218-221.PubMedGoogle Scholar
  55. 55.
    Wada RK, Bradford A, Moogk M, et al. Cord blood units collected at a remote site: a collaborative endeavor to collect umbilical cord blood through the Hawaii Cord Blood Bank and store the units at the Puget Sound Blood Center. Transfusion. 2004;44:111-118.CrossRefPubMedGoogle Scholar
  56. 56.
    Solves P, Moraga R, Saucedo E, et al. Comparison between two strategies for umbilical cord blood collection. Bone Marrow Transplant. 2003;31:269-273.CrossRefPubMedGoogle Scholar
  57. 57.
    Guttridge M, Sidders C, Booth-Davey E, Pamphilon D, Watt SM. Factors affecting volume reduction and red blood cell depletion of bone marrow using the Cobe Spectra cell separator prior to haematopoietic stem cell transplantation. Bone Marrow Transplant. 2006;38:175-181.CrossRefPubMedGoogle Scholar
  58. 58.
    RodrÍguez L, Azqueta C, Azzalin S, García J, Querol S. Washing of cord blood grafts after thawing: high cell recovery using an automated and closed system. Vox Sang. 2004;87:165-172.CrossRefPubMedGoogle Scholar
  59. 59.
    Stanworth S, Warwick R, Fehily D, et al. An international survey of unrelated umbilical cord blood banking. Vox Sang. 2001;80:236-243.CrossRefPubMedGoogle Scholar
  60. 60.
    Ljungman P, Urbano-Ispizua A, Cavazzana-Calvo et al. Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: definitions and current practice in Europe. Bone Marrow Transplant. 2006;37:439–449Google Scholar
  61. 61.
    Bielorai B, Hughes MR, Auerbach AD, et al. Successful umbilical cord blood transplantation for Fanconi anemia using preimplantation genetic diagnosis for HLA-matched donor. Am J Hematol. 2004;77:397-399.CrossRefPubMedGoogle Scholar
  62. 62.
    Grewal SS, Kahn JP, MacMillan ML, Ramsay NK, Wagner JE. Successful hematopoietic stem cell transplantation for Fanconi anemia from an unaffected HLA-genotype-identical sibling selected using preimplantation genetic diagnosis. Blood. 2004;103:1147-1151.CrossRefPubMedGoogle Scholar
  63. 63.
    Van de Velde H, Georgiou I, De Rycke M, et al. Novel universal approach for preimplantation genetic diagnosis of beta-thalassaemia in combination with HLA matching of embryos. Hum Reprod. 2004;19:700-708.CrossRefPubMedGoogle Scholar
  64. 64.
    Bhattacharya A, Slatter MA, Chapman CE, et al. Single centre experience of umbilical cord stem cell transplantation for primary immunodeficiency. Bone Marrow Transplant. 2005;36:295-299.CrossRefPubMedGoogle Scholar
  65. 65.
    Broxmeyer HE, Cooper S. High-efficiency recovery of immature haematopoietic progenitor cells with extensive proliferative capacity from human cord blood cryopreserved for 10 years. Clin Exp Immunol. 1997;107:45-53.PubMedGoogle Scholar
  66. 66.
    Krishnamurti L, Abel S, Maiers M, Flesch S. Availability of unrelated donors for hematopoietic stem cell transplantation for hemoglobinopathies. Bone Marrow Transplant. 2003;31:547-550.CrossRefPubMedGoogle Scholar
  67. 67.
    Gluckman E, Rocha V. Cord blood transplantation for children with acute leukaemia: a Eurocord registry analysis. Blood Cells Mol Dis. 2004;33:271-273.CrossRefPubMedGoogle Scholar
  68. 68.
    Locatelli F, Rocha V, Reed W, et al. Related umbilical cord blood transplantation in patients with thalassemia and sickle cell disease. Blood. 2003;101:2137-2143.CrossRefPubMedGoogle Scholar
  69. 69.
    Rocha V, Cornish J, Sievers EL, et al. Comparison of unrelated bone marrow and umbilical cord blood transplants in children with acute leukemia. Blood. 2001;97:2962-2971.CrossRefPubMedGoogle Scholar
  70. 70.
    Bitan M, Or R, Shapira MY, et al. Fludarabine-based reduced intensity conditioning for stem cell transplantation of Fanconi anemia patients from fully matched related and unrelated donors. Biol Blood Marrow Transplant. 2006;12:712-718.CrossRefPubMedGoogle Scholar
  71. 71.
    Rubinstein P, Taylor PE, Scaradavou A, et al. Unrelated placental cord blood for bone marrow reconstitution organisation of the placental blood programme. Blood Cells. 2004;20:587-600.Google Scholar
  72. 72.
  73. 73.
    Ballen KK, Barker JN, Stewart SK, Greene MF, Lane TA. American Society of Blood and Marrow Transplantation. Collection and preservation of cord blood for personal use. Biol Blood Marrow Transplant. 2008;14(3):356-363.CrossRefPubMedGoogle Scholar
  74. 74.
    Hunt CJ, Pegg DE, Armitage SE. Optimising cryopreservation protocols for haematopoietic progenitor cells: a methodological approach for umbilical cord blood. Cryo Lett. 2006;27(2):73-86.Google Scholar
  75. 75.
    Zhang Y, Fisher N, Newey SE, et al. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev. 2009;18(2):359-375.CrossRefPubMedGoogle Scholar
  76. 76.
    Martin-Rendon E, Sweeney D, Lu F, Girdlestone J, Navarrete C, Watt SM. 5-Azacytidine-treated human mesenchymal stem/progenitor cells derived from umbilical cord, cord blood and bone marrow do not generate cardiomyocytes in vitro at high frequencies. Vox Sang. 2008;95(2):137-148.CrossRefPubMedGoogle Scholar
  77. 77.
    Smythe J, Fox A, Fisher N, Frith E, Harris AL, Watt SM. Measuring angiogenic cytokines, circulating endothelial cells, and endothelial progenitor cells in peripheral blood and cord blood: VEGF and CXCL12 correlate with the number of circulating endothelial progenitor cells in peripheral blood. Tissue Eng Part C Methods. 2008;14(1):59-67.CrossRefPubMedGoogle Scholar
  78. 78.
    Fox A, Gal S, Fisher N, et al. Quantification of circulating cell-free plasma DNA and endothelial gene RNA in patients with burns and relation to acute thermal injury. Burns. 2008;34(6):809-816.CrossRefPubMedGoogle Scholar
  79. 79.
    Martin-Rendon E, Hale SJ, Ryan D, et al. Transcriptional profiling of human cord blood CD133+ and cultured bone marrow mesenchymal stem cells in response to hypoxia. Stem Cells. 2007;25(4):1003-1012.CrossRefPubMedGoogle Scholar
  80. 80.
    Cheung JO, Casals-Pascual C, Roberts DJ, Watt SM. A small-scale serum-free liquid cell culture model of erythropoiesis to assess the effects of exogenous factors. J Immunol Methods. 2007;319(1–2):104-117.CrossRefPubMedGoogle Scholar
  81. 81.
    Casals-Pascual C, Kai O, Cheung JO, et al. Suppression of erythropoiesis in malarial anemia is associated with hemozoin in vitro and in vivo. Blood. 2006;108(8):2569-2577.CrossRefPubMedGoogle Scholar
  82. 82.
    Forde S, Tye BJ, Newey SE, et al. Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133+ cells. Blood. 2007;109(5):1825-1833.CrossRefPubMedGoogle Scholar
  83. 83.
    Jorgensen-Tye B, Levesque JP, Royle L, et al. Epitope recognition of antibodies that define the sialomucin, endolyn (CD164), a negative regulator of haematopoiesis. Tissue Antigens. 2005;65(3):220-239.CrossRefPubMedGoogle Scholar
  84. 84.
    McGuckin CP, Pearce D, Forraz N, Tooze JA, Watt SM, Pettengell R. Multiparametric analysis of immature cell populations in umbilical cord blood and bone marrow. Eur J Haematol. 2003;71(5):341-350.CrossRefPubMedGoogle Scholar
  85. 85.
    McGuckin CP, Forraz N, Baradez MO, et al. Colocalization analysis of sialomucins CD34 and CD164. Stem Cells. 2003;21(2):162-170.CrossRefPubMedGoogle Scholar
  86. 86.
    Zannettino AC, Roubelakis M, Welldon KJ, et al. Novel mesenchymal and haematopoietic cell isoforms of the SHP-2 docking receptor, PZR: identification, molecular cloning and effects on cell migration. Biochem J. 2003;370(pt 2):537-549.CrossRefPubMedGoogle Scholar
  87. 87.
    Connolly NP, Jones M, Watt SM. Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions. Br J Haematol. 2002;119(1):221-238.CrossRefPubMedGoogle Scholar
  88. 88.
    Hunt CJ, Armitage SE, Pegg DE. Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing. Cryobiology. 2003;46(1):76-87.CrossRefPubMedGoogle Scholar
  89. 89.
    Hunt CJ, Armitage SE, Pegg DE. Cryopreservation of umbilical cord blood: 1. Osmotically inactive volume, hydraulic conductivity and permeability of CD34(+) cells to dimethyl sulphoxide. Cryobiology. 2003;46(1):61-75.CrossRefPubMedGoogle Scholar
  90. 90.
    Tunstall-Pedoe O, Roy A, Karadimitris A, et al. Abnormalities in the myeloid progenitor compartment in Down syndrome fetal liver precede acquisition of GATA1 mutations. Blood. 2008;112(12):4507-4511.CrossRefPubMedGoogle Scholar
  91. 91.
    Hamlett I, Draper J, Strouboulis J, Iborra F, Porcher C, Vyas P. Characterization of megakaryocyte GATA1-interacting proteins: the corepressor ETO2 and GATA1 interact to ­regulate terminal megakaryocyte. Blood. 2008;112(7):2738-2749.CrossRefPubMedGoogle Scholar
  92. 92.
    Gupta R, Hong D, Iborra F, Sarno S, Enver T. NOV (CCN3) functions as a regulator of human hematopoietic stem or progenitor cells. Science. 2007;316(5824):590-593.CrossRefPubMedGoogle Scholar
  93. 93.
    Hill AJ, Zwart I, Tam HH, et al. Human umbilical cord blood-derived mesenchymal stem cells do not differentiate into neural cells types or integrate into the retina after intravitreal grafting in neonatal rats. Stem Cells Dev. 2009;18(3):399-409.CrossRefPubMedGoogle Scholar
  94. 94.
    Zwart I, Hill AJ, Girdlestone J, et al. Analysis of neural potential of human umbilical cord blood-derived multipotent mesenchymal stem cells in response to a range of neurogenic stimuli. J Neurosci Res. 2008;86(9):1902-1915.CrossRefPubMedGoogle Scholar
  95. 95.
    Manca MF, Zwart I, Beo J, et al. Characterization of mesenchymal stromal cells derived from full-term umbilical cord blood. Cytotherapy. 2008;10(1):54-68.CrossRefPubMedGoogle Scholar
  96. 96.
    Tisato V, Naresh K, Girdlestone J, Navarrete C, Dazzi F. Mesenchymal stem cells of cord blood origin are effective at preventing but not treating graft-versus-host disease. Leukemia. 2007;21(9):1992-1999.CrossRefPubMedGoogle Scholar
  97. 97.
    Friese MA, Jensen LT, Willcox N, Fugger L. Humanized mouse models for organ-specific autoimmune diseases. Curr Opin Immunol. 2006;18(6):704-709.CrossRefPubMedGoogle Scholar

Copyright information

© Springer London 2011

Authors and Affiliations

  • Suzanne M. Watt
    • 1
    • 2
  • Katherine Coldwell
  • Jon Smythe
  1. 1.Stem Cell Laboratory, NHS Blood and TransplantJohn Radcliffe HospitalOxfordUK
  2. 2.Nuffield Department of Clinical Laboratory SciencesUniversity of OxfordOxfordUK

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