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Oral and Intestinal Bacteroidetes

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Molecular Typing in Bacterial Infections

Part of the book series: Infectious Disease ((ID))

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Abstract

Bacteroidetes are a phylum of bacteria including the class Bacteroides which consists of the genera Bacteroides, Porphyromonas, and Prevotella. Whereas the genus Bacteroides is discussed and subjected with infections originating from the intestinal tract, Porphyromonas is clearly more associated with oral or vaginal infections. With both genera (and related such as Bilophila or Prevotella) different methods are described and discussed exemplarily. Critical to this development, however, is a proper understanding and application of the methodologies and knowledge of their limitations. In this chapter, molecular tools based on ITS (Internal Transcribed Spacer) amplification and sequencing as well as PCR fingerprint techniques are described along with examples showing ways to analyze the datasets. Both methods allow the identification of almost any given bacterial species or strain in pure culture or even directly in clinical samples in a sensitive and reproducible way. This chapter is complemented by discussing potential pitfalls that should be taken into consideration for producing proper results along with referring the reader to pertinent literature that will allow an individual deepening into the concept of molecular-typing in clinical bacteriology.

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Author information

Authors and Affiliations

  1. Institute of Medical Microbiology and Infectious Epidemiology (at time of experiments), University of Leipzig, Leipzig, Germany

    Marina C. Claros

  2. Division of Oral Microbiology and Immunology, Department of Operative and Preventive Dentistry & Periodontology and Department of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany

    Georg Conrads

  3. Division of Oral Microbiology and Immunology, University Hospital (RWTH), Pauwelsstrasse 30, Aachen, 52057, Germany

    Georg Conrads

Authors
  1. Marina C. Claros
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  2. Georg Conrads
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Correspondence to Georg Conrads .

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Editors and Affiliations

  1. Instituto Nacional de Controle de, Qualidade em Saúde, Fundação Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Rio de Janeiro, 21045-900, Rio de Janeiro, Brazil

    Ivano de Filippis

  2. Microbiology, BioReliance Corporation, Broschart Road 14920, Rockville, 20850, Maryland, USA

    Marian L. McKee

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Claros, M.C., Conrads, G. (2013). Oral and Intestinal Bacteroidetes . In: de Filippis, I., McKee, M. (eds) Molecular Typing in Bacterial Infections. Infectious Disease. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-185-1_7

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  • DOI: https://doi.org/10.1007/978-1-62703-185-1_7

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