Abstract
Eukaryotic cells contain three distinct cytoskeletal filament systems, including actin, that exhibit very different assembly properties, supramolecular architectures, dynamic behavior, and mechanical properties. Actin, which is involved in a plethora of functions, is the most abundant protein in most cell types and is impressively conserved across species. The highest concentrations of actin (about 20% of total protein) are found as stable microfilament systems assembled within myofibrillar contractile structures in striated muscles. In addition to its specialized role in muscle contraction, actin is present in all muscle and nonmuscle cells, where it plays a variety of roles thanks to its ability to assemble and disassemble depending on cell requirements. Actin plays an important role in maintaining cell structure and function by conferring mechanical strength and enabling intracellular contraction and/or tension. In nonmuscle cells, microfilaments are involved in cell motility and cytokinesis. The dynamics of the actin cytoskeleton are maintained by two factors: (1) the ability of actin to undergo reversible transformation from the monomeric state (G-actin) to the polymeric state (F-actin) and (2) the interaction of actin with actin-binding proteins (ABPs), that can inhibit or stimulate actin polymerization, sever the polymers, cross-link actin filaments into bundles or in filamentous three-dimensional networks, and bind them to cell membranes. Considering the multiple cellular functions of actin, alterations in the organization of microfilaments will result in disorganized cell arrangement and orientation, uncontrolled cell growth, and abnormal responses to the environment. Higher vertebrates express six different highly conserved actin isoforms. Over the last decades, numerous studies have tried to elucidate the specific expressions, localizations, regulations, properties, and functions of the different isoactins. The understanding of their specific underlying mechanisms would be of major relevance not only for fundamental research but also for clinical applications, since modulations of actin isoforms are directly or indirectly correlated with severe pathologies.
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Notes
- 1.
Arginylation is a post-translational modification mediated by Arg-tRNA protein transferase (ATE1) which transfers arginine (Arg) from tRNA onto proteins.
- 2.
In embryonic chicken and mouse muscles, α-CAA still accounts for 90% and 60–70%, respectively.
- 3.
- 4.
The transgenically expressed γ-SMA reduces cardiac contractility even in wild-type and heterozygous mice.
- 5.
The differences in amino acid composition between α-CAA and γ-SMA could affect the closure or the opening of the cleft and thus the transition from the weak to the strong binding state.
References
Soufo HJ, Graumann PL (2003) Actin-like proteins MreB and Mbl from Bacillus subtilis are required for bipolar positioning of replication origins. Curr Biol 13:1916–1920
Wang S, Arellano-Santoyo H, Combs PA, Shaevitz JW (2010) Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria. Proc Natl Acad Sci U S A 107:9182–9185
Vandekerckhove J, Weber K (1978a) At least six different actins are expressed in a higher mammal: An analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol 126:783–802
Harborth J, Elbashir SM, Bechert K, Tuschl T, Weber K (2001) Identification of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Sci 114:4557–4565
Straub FB (1942) Actin. Stud Szeged 2:3–15
Hennessey ES, Drummond DR, Sparrow JC (1993) Molecular genetics of actin function. Biochem J 291(3):657–671
Grazi E, Magri E (1979) Phosphorylation of actin and removal of its inhibitory activity on pancreatic DNAase I by liver plasma membranes. FEBS Lett 104:284–286
Wegner A, Aktories K (1988) ADP-ribosylated actin caps the barbed ends of actin filaments. J Biol Chem 263:13739-13742
Sheterline P, Clayton J, Sparrow J (1995) Actin. Protein Profile 2:1–103
Saha S, Mundia MM, Zhang F, Demers RW, Korobova F, Svitkina T, Perieteanu AA, Dawson JF, Kashina A (2010) Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins. Mol Biol Cell 21:1350–1361
Karakozova M, Kozak M, Wong CC, Bailey AO, Yates JR, 3rd, Mogilner A, Zebroski H, Kashina A (2006) Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313:192–196
Murakami K, Yasunaga T, Noguchi TQ, Gomibuchi Y, Ngo KX, Uyeda TQ, Wakabayashi T (2010) Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release. Cell 143:275–287
Brooks FJ, Carlsson AE (2009) Nonequilibrium actin polymerization treated by a truncated rate-equation method. Phys Rev E Stat Nonlin Soft Matter Phys 79:031914
Galinska-Rakoczy A, Wawro B, Strzelecka-Golaszewska H (2009) New aspects of the spontaneous polymerization of actin in the presence of salts. J Mol Biol 387:869–882
Carlier MF (1989) Role of nucleotide hydrolysis in the dynamics of actin filaments and microtubules. Int Rev Cytol 115:139–170
Wegner A, Engel J (1975) Kinetics of the cooperative association of actin to actin filaments. Biophys Chem 3:215–225
Murphy DB, Gray RO, Grasser WA, Pollard TD (1988) Direct demonstration of actin filament annealing in vitro. J Cell Biol 106:1947–1954
Fass J, Pak C, Bamburg J, Mogilner A (2008) Stochastic simulation of actin dynamics reveals the role of annealing and fragmentation. J Theor Biol 252:173–183
Andrianantoandro E, Blanchoin L, Sept D, McCammon JA, Pollard TD (2001) Kinetic mechanism of end-to-end annealing of actin filaments. J Mol Biol 312:721–730
Kueh HY, Mitchison TJ (2009) Structural plasticity in actin and tubulin polymer dynamics. Science 325:960–963
Engel J, Fasold H, Hulla FW, Waechter F, Wegner A (1977) The polymerization reaction of muscle actin. Mol Cell Biochem 18:3–13
Li X, Kierfeld J, Lipowsky R (2009) Actin polymerization and depolymerization coupled to cooperative hydrolysis. Phys Rev Lett 103:048102
Muhlrad A, Ringel I, Pavlov D, Peyser YM, Reisler E (2006) Antagonistic effects of cofilin, beryllium fluoride complex, and phalloidin on subdomain 2 and nucleotide-binding cleft in F-actin. Biophys J 91:4490–4499
Kardos R, Pozsonyi K, Nevalainen E, Lappalainen P, Nyitrai M, Hild G (2009) The effects of ADF/cofilin and profilin on the conformation of the ATP-binding cleft of monomeric actin. Biophys J 96:2335–2343
Laham LE, Lamb JA, Allen PG, Janmey PA (1993) Selective binding of gelsolin to actin monomers containing ADP. J Biol Chem 268:14202–14207
Allen PG, Laham LE, Way M, Janmey PA (1996a) Binding of phosphate, aluminum fluoride, or beryllium fluoride to F-actin inhibits severing by gelsolin. J Biol Chem 271:4665–4670
dos Remedios CG, Chhabra D, Kekic M, Dedova IV, Tsubakihara M, Berry DA, Nosworthy NJ (2003) Actin binding proteins: regulation of cytoskeletal microfilaments. Physiol Rev 83:433–473
Pollard TD, Cooper JA (1986) Actin and actin-binding proteins. A critical evaluation of mechanisms and functions. Annu Rev Biochem 55:987–1035
Amos LA, Schlieper D (2005) Microtubules and maps. Adv Protein Chem 71:257–298
Green KJ, Bohringer M, Gocken T, Jones JC (2005) Intermediate filament associated proteins. Adv Protein Chem 70:143–202
Winder SJ, Ayscough KR (2005) Actin-binding proteins. J Cell Sci 118:651–654
Lee H, Ferrer JM, Lang MJ, Kamm RD (2010) Molecular origin of strain softening in cross-linked F-actin networks. Phys Rev E Stat Nonlin Soft Matter Phys 82:011919
Stricker J, Falzone T, Gardel ML (2010) Mechanics of the F-actin cytoskeleton. J Biomech 43:9–14
Mizuno D, Tardin C, Schmidt CF, Mackintosh FC (2007) Nonequilibrium mechanics of active cytoskeletal networks. Science 315:370–373
Vandekerckhove J, Weber K (1978b) Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins. Proc Natl Acad Sci U S A 75:1106–1110
Kabsch W, Vandekerckhove J (1992) Structure and function of actin. Annu Rev Biophys Biomol Struct 21:49–76
Gunning P, Ponte P, Kedes L, Eddy R, Shows T (1984a) Chromosomal location of the co-expressed human skeletal and cardiac actin genes. Proc Natl Acad Sci U S A 81:1813–1817
Hightower RC, Meagher RB (1986) The molecular evolution of actin. Genetics 114:315–332
Obinata T, Maruyama K, Sugita H, Kohama K, Ebashi S (1981) Dynamic aspects of structural proteins in vertebrate skeletal muscle. Muscle Nerve 4:456–488
Vandekerckhove J, Weber K (1978c) Comparison of the amino acid sequences of three tissue-specific cytoplasmic actins with rabbit skeletal muscle actin (proceedings). Arch Int Physiol Biochim 86:891–892
Vandekerckhove J, Weber K (1979a) Amino-acid sequence analysis of the amino-terminal tryptic peptides of different actins from the same mammal (proceedings). Arch Int Physiol Biochim 87:210–212
Vandekerckhove J, Weber K (1979b) The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation. Differentiation 14:123–133
Garrels JI, Gibson W (1976) Identification and characterization of multiple forms of actin. Cell 9:793–805
Rubenstein PA, Spudich JA (1977) Actin microheterogeneity in chick embryo fibroblasts. Proc Natl Acad Sci U S A 74:120–123
Schutt CE, Myslik JC, Rozycki MD, Goonesekere NC, Lindberg U (1993) The structure of crystalline profilin-beta-actin. Nature 365:810–816
Mounier N, Sparrow JC (1997) Structural comparisons of muscle and nonmuscle actins give insights into the evolution of their functional differences. J Mol Evol 44:89–97
Allen PG, Shuster CB, Kas J, Chaponnier C, Janmey PA, Herman IM (1996b) Phalloidin binding and rheological differences among actin isoforms. Biochemistry 35:14062–14069
Orban J, Lorinczy D, Nyitrai M, Hild G (2008) Nucleotide dependent differences between the alpha-skeletal and alpha-cardiac actin isoforms. Biochem Biophys Res Commun 368:696–702
Khaitlina S, Hinssen H (2008) Difference in polymerization and steady-state dynamics of free and gelsolin-capped filaments formed by alpha- and beta-isoactins. Arch Biochem Biophys 477:279–284
Bergeron SE, Zhu M, Thiem SM, Friderici KH, Rubenstein PA (2010) Ion-dependent polymerization differences between mammalian beta- and gamma-nonmuscle actin isoforms. J Biol Chem 285:16087–16095
Strzelecka-Golaszewska H, Sobieszek A (1981) Activation of smooth muscle myosin by smooth and skeletal muscle actins. FEBS Lett 134:197–202
Mossakowska M, Strzelecka-Golaszewska H (1985) Identification of amino acid substitutions differentiating actin isoforms in their interaction with myosin. Eur J Biochem 153:373–381
Orlova A, Yu X, Egelman EH (1994) Three-dimensional reconstruction of a co-complex of F-actin with antibody Fab fragments to actin’s NH2 terminus. Biophys J 66:276–285
Larsson H, Lindberg U (1988) The effect of divalent cations on the interaction between calf spleen profilin and different actins. Biochim Biophys Acta 953:95–105
Ohshima S, Abe H, Obinata T (1989) Isolation of profilin from embryonic chicken skeletal muscle and evaluation of its interaction with different actin isoforms. J Biochem 105:855–857
Weber A, Nachmias VT, Pennise CR, Pring M, Safer D (1992) Interaction of thymosin beta 4 with muscle and platelet actin: implications for actin sequestration in resting platelets. Biochemistry 31:6179–6185
Prassler J, Stocker S, Marriott G, Heidecker M, Kellermann J, Gerisch G (1997) Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes. Mol Biol Cell 8:83–95
Namba Y, Ito M, Zu Y, Shigesada K, Maruyama K (1992) Human T cell L-plastin bundles actin filaments in a calcium-dependent manner. J Biochem 112:503–507
Shuster CB, Lin AY, Nayak R, Herman IM (1996) Beta cap73: a novel beta actin-specific binding protein. Cell Motil Cytoskeleton 35:175–187
Shuster CB, Herman IM (1995) Indirect association of ezrin with F-actin: isoform specificity and calcium sensitivity. J Cell Biol 128:837–848
Yao X, Cheng L, Forte JG (1996) Biochemical characterization of ezrin-actin interaction. J Biol Chem 271:7224–7229
Winder SJ, Hemmings L, Maciver SK, Bolton SJ, Tinsley JM, Davies KE, Critchley DR, Kendrick-Jones J (1995) Utrophin actin binding domain: analysis of actin binding and cellular targeting. J Cell Sci 108(1):63–71
Tzima E, Trotter PJ, Orchard MA, Walker JH (2000) Annexin V relocates to the platelet cytoskeleton upon activation and binds to a specific isoform of actin. Eur J Biochem 267:4720–4730
Gallant C, Appel S, Graceffa P, Leavis PC, Lin JJ, Gunning PW, Schevzov G, Chaponnier C, Degnore J, Lehman W, Morgan KG (2011) Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol 300(6):1356–1365
Storti RV, Coen DM, Rich A (1976) Tissue-specific forms of actin in the developing chick. Cell 8:521–527
Wiens D, Spooner BS (1983) Actin isotype biosynthetic transitions in early cardiac organogenesis. Eur J Cell Biol 30:60–66
Owens GK, Thompson MM (1986) Developmental changes in isoactin expression in rat aortic smooth muscle cells in vivo. Relationship between growth and cytodifferentiation. J Biol Chem 261:13373–13380
Gunning P, Hardeman E, Jeffrey P, Weinberger R (1998a) Creating intracellular structural domains: spatial segregation of actin and tropomyosin isoforms in neurons. Bioessays 20:892–900
Gunning P, Weinberger R, Jeffrey P, Hardeman E (1998b) Isoform sorting and the creation of intracellular compartments. Annu Rev Cell Dev Biol 14:339–372
Gunning P, Mohun T, Ng SY, Ponte P, Kedes L (1984b) Evolution of the human sarcomeric-actin genes: evidence for units of selection within the 3’ untranslated regions of the mRNAs. J Mol Evol 20:202–214
Yaffe D, Nudel U, Mayer Y, Neuman S (1985) Highly conserved sequences in the 3’ untranslated region of mRNAs coding for homologous proteins in distantly related species. Nucleic Acids Res 13:3723–3737
Parker TG, Chow KL, Schwartz RJ, Schneider MD (1992) Positive and negative control of the skeletal alpha-actin promoter in cardiac muscle. A proximal serum response element is sufficient for induction by basic fibroblast growth factor (FGF) but not for inhibition by acidic FGF. J Biol Chem 267:3343–3350
Blank RS, McQuinn TC, Yin KC, Thompson MM, Takeyasu K, Schwartz RJ, Owens GK (1992) Elements of the smooth muscle alpha-actin promoter required in cis for transcriptional activation in smooth muscle. Evidence for cell type-specific regulation. J Biol Chem 267:984–989
Foster DN, Min B, Foster LK, Stoflet ES, Sun S, Getz MJ, Strauch AR (1992) Positive and negative cis-acting regulatory elements mediate expression of the mouse vascular smooth muscle alpha-actin gene. J Biol Chem 267:11995–12003
Treisman R, Alberts AS, Sahai E (1998) Regulation of SRF activity by Rho family GTPases. Cold Spring Harb Symp Quant Biol 63:643–651
Miano JM (2003) Serum response factor: toggling between disparate programs of gene expression. J Mol Cell Cardiol 35:577–593
Posern G, Treisman R (2006) Actin’ together: serum response factor, its cofactors and the link to signal transduction. Trends Cell Biol 16:588–596
Carson JA, Fillmore RA, Schwartz RJ, Zimmer WE (2000) The smooth muscle gamma-actin gene promoter is a molecular target for the mouse bagpipe homologue, mNkx3–1, and serum response factor. J Biol Chem 275:39061–39072
Kuwahara K, Barrientos T, Pipes GC, Li S, Olson EN (2005) Muscle-specific signaling mechanism that links actin dynamics to serum response factor. Mol Cell Biol 25:3173–3181
Chen CY, Croissant J, Majesky M, Topouzis S, McQuinn T, Frankovsky MJ, Schwartz RJ (1996) Activation of the cardiac alpha-actin promoter depends upon serum response factor, Tinman homologue, Nkx-2.5, and intact serum response elements. Dev Genet 19:119–130
Mack CP, Owens GK (1999) Regulation of smooth muscle alpha-actin expression in vivo is dependent on CArG elements within the 5’ and first intron promoter regions. Circ Res 84:852–861
Miralles F, Visa N (2006) Actin in transcription and transcription regulation. Curr Opin Cell Biol 18:261–266
Singer RH (1992) The cytoskeleton and mRNA localization. Curr Opin Cell Biol 4:15–19
Gunning P, Weinberger R, Jeffrey P (1997) Actin and tropomyosin isoforms in morphogenesis. Anat Embryol (Berl) 195:311–315
Martin KC, Ephrussi A (2009) mRNA localization: gene expression in the spatial dimension. Cell 136:719–730
Latham VM, Jr., Kislauskis EH, Singer RH, Ross AF (1994) Beta-actin mRNA localization is regulated by signal transduction mechanisms. J Cell Biol 126:1211–1219
Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, Bertrand E (2003) Single mRNA molecules demonstrate probabilistic movement in living mammalian cells. Curr Biol 13:161–167
Oleynikov Y, Singer RH (2003) Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization. Curr Biol 13:199–207
Kislauskis EH, Li Z, Singer RH, Taneja KL (1993) Isoform-specific 3’-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments. J Cell Biol 123:165–172
Ross AF, Oleynikov Y, Kislauskis EH, Taneja KL, Singer RH (1997) Characterization of a beta-actin mRNA zipcode-binding protein. Mol Cell Biol 17:2158–2165
Huttelmaier S, Zenklusen D, Lederer M, Dictenberg J, Lorenz M, Meng X, Bassell GJ, Condeelis J, Singer RH (2005) Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1. Nature 438:512–515
Hill MA, Gunning P (1993) Beta and gamma actin mRNAs are differentially located within myoblasts. J Cell Biol 122:825–832
Hannan AJ, Gunning P, Jeffrey PL, Weinberger RP (1998) Structural compartments within neurons: developmentally regulated organization of microfilament isoform mRNA and protein. Mol Cell Neurosci 11:289–304
Fulton AB (1993) Spatial organization of the synthesis of cytoskeletal proteins. J Cell Biochem 52:148–152
Peng I, Fischman DA (1991) Post-translational incorporation of actin into myofibrils in vitro: evidence for isoform specificity. Cell Motil Cytoskeleton 20:158–168
Kashina AS (2006) Differential arginylation of actin isoforms: the mystery of the actin N-terminus. Trends Cell Biol 16:610–615
Wong CC, Xu T, Rai R, Bailey AO, Yates JR, 3rd, Wolf YI, Zebroski H, Kashina A (2007) Global analysis of posttranslational protein arginylation. PLoS Biol 5:e258
Zhang F, Saha S, Shabalina SA, Kashina A (2010) Differential arginylation of actin isoforms is regulated by coding sequence-dependent degradation. Science 329:1534–1537
Rai R, Wong CC, Xu T, Leu NA, Dong DW, Guo C, McLaughlin KJ, Yates JR, 3rd, Kashina A (2008) Arginyltransferase regulates alpha cardiac actin function, myofibril formation and contractility during heart development. Development 135:3881–3889
Lambrechts A, Van Troys M, Ampe C (2004) The actin cytoskeleton in normal and pathological cell motility. Int J Biochem Cell Biol 36:1890–1909
Chaponnier C, Gabbiani G (2004) Pathological situations characterized by altered actin isoform expression. J Pathol 204:386–395
Tondeleir D, Vandamme D, Vandekerckhove J, Ampe C, Lambrechts A (2009) Actin isoform expression patterns during mammalian development and in pathology: insights from mouse models. Cell Motil Cytoskeleton 66:798–815
Perrin BJ, Ervasti JM (2010) The actin gene family: function follows isoform. Cytoskeleton (Hoboken) 67:630–634
Gunning P, Ponte P, Blau H, Kedes L (1983) Alpha-skeletal and alpha-cardiac actin genes are coexpressed in adult human skeletal muscle and heart. Mol Cell Biol 3:1985–1995
Paterson BM, Eldridge JD (1984) Alpha-Cardiac actin is the major sarcomeric isoform expressed in embryonic avian skeletal muscle. Science 224:1436–1438
Vandekerckhove J, Bugaisky G, Buckingham M (1986) Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. A quantitative determination of the two actin isoforms. J Biol Chem 261:1838–1843
Gunning P, Hardeman E, Wade R, Ponte P, Bains W, Blau HM, Kedes L (1987) Differential patterns of transcript accumulation during human myogenesis. Mol Cell Biol 7:4100–4114
McHugh KM, Crawford K, Lessard JL (1991) A comprehensive analysis of the developmental and tissue-specific expression of the isoactin multigene family in the rat. Dev Biol 148:442–458
Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 103:2787–2796
Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton 27:108–116
Dugina V, Zwaenepoel I, Gabbiani G, Clement S, Chaponnier C (2009) Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity. J Cell Sci 122:2980–2988
Franke WW, Stehr S, Stumpp S, Kuhn C, Heid H, Rackwitz HR, Schnolzer M, Baumann R, Holzhausen HJ, Moll R (1996) Specific immunohistochemical detection of cardiac/fetal alpha-actin in human cardiomyocytes and regenerating skeletal muscle cells. Differentiation 60:245–250
Clement S, Orlandi A, Bocchi L, Pizzolato G, Foschini MP, Eusebi V, Gabbiani G (2003) Actin isoform pattern expression: a tool for the diagnosis and biological characterization of human rhabdomyosarcoma. Virchows Arch 442:31–38
Driesen RB, Verheyen FK, Debie W, Blaauw E, Babiker FA, Cornelussen RN, Ausma J, Lenders MH, Borgers M, Chaponnier C, Ramaekers FC (2009) Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13:896–908
Clement S, Chaponnier C, Gabbiani G (1999) A subpopulation of cardiomyocytes expressing alpha-skeletal actin is identified by a specific polyclonal antibody. Circ Res 85:e51–58
Hanft LM, Rybakova IN, Patel JR, Rafael-Fortney JA, Ervasti JM (2006) Cytoplasmic gamma-actin contributes to a compensatory remodeling response in dystrophin-deficient muscle. Proc Natl Acad Sci U S A 103:5385–5390
Sawtell NM, Lessard JL (1989) Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes. J Cell Biol 109:2929–2937
Minty AJ, Alonso S, Caravatti M, Buckingham ME (1982) A fetal skeletal muscle actin mRNA in the mouse and its identity with cardiac actin mRNA. Cell 30:185–192
Carrier L, Boheler KR, Chassagne C, de la Bastie D, Wisnewsky C, Lakatta EG, Schwartz K (1992) Expression of the sarcomeric actin isogenes in the rat heart with development and senescence. Circ Res 70:999–1005
Bertola LD, Ott EB, Griepsma S, Vonk FJ, Bagowski CP (2008) Developmental expression of the alpha-skeletal actin gene. BMC Evol Biol 8:166
Ruzicka DL, Schwartz RJ (1988) Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation. J Cell Biol 107:2575–2586
Woodcock-Mitchell J, Mitchell JJ, Low RB, Kieny M, Sengel P, Rubbia L, Skalli O, Jackson B, Gabbiani G (1988) Alpha-smooth muscle actin is transiently expressed in embryonic rat cardiac and skeletal muscles. Differentiation 39:161–166
Hewett TE, Grupp IL, Grupp G, Robbins J (1994) Alpha-skeletal actin is associated with increased contractility in the mouse heart. Circ Res 74:740–746
Clement S, Stouffs M, Bettiol E, Kampf S, Krause KH, Chaponnier C, Jaconi M (2007) Expression and function of alpha-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation. J Cell Sci 120:229–238
Moll R, Holzhausen HJ, Mennel HD, Kuhn C, Baumann R, Taege C, Franke WW (2006) The cardiac isoform of alpha-actin in regenerating and atrophic skeletal muscle, myopathies and rhabdomyomatous tumors: an immunohistochemical study using monoclonal antibodies. Virchows Arch 449:175–191
Kumar A, Crawford K, Close L, Madison M, Lorenz J, Doetschman T, Pawlowski S, Duffy J, Neumann J, Robbins J, Boivin GP, O’Toole BA, Lessard JL (1997) Rescue of cardiac alpha-actin-deficient mice by enteric smooth muscle gamma-actin. Proc Natl Acad Sci U S A 94:4406–4411
Martin AF, Phillips RM, Kumar A, Crawford K, Abbas Z, Lessard JL, de Tombe P, Solaro RJ (2002) Ca(2+) activation and tension cost in myofilaments from mouse hearts ectopically expressing enteric gamma-actin. Am J Physiol Heart Circ Physiol 283:H642–649
Blau HM, Baltimore D (1991) Differentiation requires continuous regulation. J Cell Biol 112:781–783
Weintraub H, Tapscott SJ, Davis RL, Thayer MJ, Adam MA, Lassar AB, Miller AD (1989) Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. Proc Natl Acad Sci U S A 86:5434–5438
Sassoon D, Lyons G, Wright WE, Lin V, Lassar A, Weintraub H, Buckingham M (1989) Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis. Nature 341:303–307
Lassar AB, Buskin JN, Lockshon D, Davis RL, Apone S, Hauschka SD, Weintraub H (1989) MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer. Cell 58:823–831
Gabbiani G, Ryan GB, Majne G (1971) Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction. Experientia 27:549–550
Majno G, Gabbiani G, Hirschel BJ, Ryan GB, Statkov PR (1971) Contraction of granulation tissue in vitro: similarity to smooth muscle. Science 173:548–550
Schurch W (1999) The myofibroblast in neoplasia. Curr Top Pathol 93:135–148
Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA (2002) Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol 3:349–363
Dugina V, Fontao L, Chaponnier C, Vasiliev J, Gabbiani G (2001) Focal adhesion features during myofibroblastic differentiation are controlled by intracellular and extracellular factors. J Cell Sci 114:3285–3296
Hinz B, Phan SH, Thannickal VJ, Galli A, Bochaton-Piallat ML, Gabbiani G (2007) The myofibroblast: one function, multiple origins. Am J Pathol 170:1807–1816
Hinz B, Gabbiani G (2010) Fibrosis: recent advances in myofibroblast biology and new therapeutic perspectives. F1000 Biol Rep 2:78
Orlandi A, Hao H, Ferlosio A, Clement S, Hirota S, Spagnoli LG, Gabbiani G, Chaponnier C (2009) Alpha actin isoforms expression in human and rat adult cardiac conduction system. Differentiation 77:360–368
Saborio JL, Segura M, Flores M, Garcia R, Palmer E (1979) Differential expression of gizzard actin genes during chick embryogenesis. J Biol Chem 254:11119–11125
Kuroda M (1985) Change of actin isomers during differentiation of smooth muscle. Biochim Biophys Acta 843:208–213
Hirai S, Hirabayashi T (1983) Developmental change of protein constituents in chicken gizzards. Dev Biol 97:483–493
Izant JG, Lazarides E (1977) Invariance and heterogeneity in the major structural and regulatory proteins of chick muscle cells revealed by two-dimensional gel electrophoresis. Proc Natl Acad Sci U S A 74:1450–1454
Stromer MH, Mayes MS, Bellin RM (2002) Use of actin isoform-specific antibodies to probe the domain structure in three smooth muscles. Histochem Cell Biol 118:291–299
Cavaille F, Leger JJ (1983) Characterization and comparison of the contractile proteins from human gravid and non-gravid myometrium. Gynecol Obstet Invest 16:341–353
Skalli O, Vandekerckhove J, Gabbiani G (1987) Actin-isoform pattern as a marker of normal or pathological smooth-muscle and fibroblastic tissues. Differentiation 33:232–238
Shynlova O, Tsui P, Dorogin A, Chow M, Lye SJ (2005) Expression and localization of alpha-smooth muscle and gamma-actins in the pregnant rat myometrium. Biol Reprod 73:773–780
Otey CA, Kalnoski MH, Bulinski JC (1987) Identification and quantification of actin isoforms in vertebrate cells and tissues. J Cell Biochem 34:113–124
Shawlot W, Deng JM, Fohn LE, Behringer RR (1998) Restricted beta-galactosidase expression of a hygromycin-lacZ gene targeted to the beta-actin locus and embryonic lethality of beta-actin mutant mice. Transgenic Res 7:95–103
Bunnell TM, Ervasti JM (2010) Delayed embryonic development and impaired cell growth and survival in Actg1 null mice. Cytoskeleton (Hoboken) 67:564–572
Watanabe H, Kislauskis EH, Mackay CA, Mason-Savas A, Marks SC, Jr (1998) Actin mRNA isoforms are differentially sorted in normal osteoblasts and sorting is altered in osteoblasts from a skeletal mutation in the rat. J Cell Sci 111(9):1287–1292
Bassell GJ, Zhang H, Byrd AL, Femino AM, Singer RH, Taneja KL, Lifshitz LM, Herman IM, Kosik KS (1998) Sorting of beta-actin mRNA and protein to neurites and growth cones in culture. J Neurosci 18:251–265
Lawrence JB, Singer RH (1986) Intracellular localization of messenger RNAs for cytoskeletal proteins. Cell 45:407–415
Shestakova EA, Singer RH, Condeelis J (2001) The physiological significance of beta-actin mRNA localization in determining cell polarity and directional motility. Proc Natl Acad Sci U S A 98:7045–7050
Otey CA, Kalnoski MH, Lessard JL, Bulinski JC (1986) Immunolocalization of the gamma isoform of nonmuscle actin in cultured cells. J Cell Biol 102:1726–1737
Schevzov G, Vrhovski B, Bryce NS, Elmir S, Qiu MR, O’Neill G M, Yang N, Verrills NM, Kavallaris M, Gunning PW (2005) Tissue-specific tropomyosin isoform composition. J Histochem Cytochem 53:557–570
Hoock TC, Newcomb PM, Herman IM (1991) Beta actin and its mRNA are localized at the plasma membrane and the regions of moving cytoplasm during the cellular response to injury. J Cell Biol 112:653–664
Belyantseva IA, Perrin BJ, Sonnemann KJ, Zhu M, Stepanyan R, McGee J, Frolenkov GI, Walsh EJ, Friderici KH, Friedman TB, Ervasti JM (2009) Gamma-actin is required for cytoskeletal maintenance but not development. Proc Natl Acad Sci U S A 106:9703–9708
Peckham M, Miller G, Wells C, Zicha D, Dunn GA (2001) Specific changes to the mechanism of cell locomotion induced by overexpression of beta-actin. J Cell Sci 114:1367–1377
Clement S, Hinz B, Dugina V, Gabbiani G, Chaponnier C (2005) The N-terminal Ac-EEED sequence plays a role in alpha-smooth-muscle actin incorporation into stress fibers. J Cell Sci 118:1395–1404
Hinz B, Gabbiani G, Chaponnier C (2002) The NH2-terminal peptide of alpha-smooth muscle actin inhibits force generation by the myofibroblast in vitro and in vivo. J Cell Biol 157:657–663
Chaponnier C, Goethals M, Janmey PA, Gabbiani F, Gabbiani G, Vandekerckhove J (1995) The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo. J Cell Biol 130:887–895
Hinz B, Dugina V, Ballestrem C, Wehrle-Haller B, Chaponnier C (2003) Alpha-smooth muscle actin is crucial for focal adhesion maturation in myofibroblasts. Mol Biol Cell 14:2508–2519
Kim HR, Gallant C, Leavis PC, Gunst SJ, Morgan KG (2008) Cytoskeletal remodeling in differentiated vascular smooth muscle is actin isoform dependent and stimulus dependent. Am J Physiol Cell Physiol. 295:C768–778
Acknowledgments
The research in the authors’ laboratories is supported by SNF grant # 310030_125320 (CC, RA) and Scientific & Technological Cooperation Programme Switzerland-Russia (CC, VD), Russian Foundation of Basic Investigation grant # 10–04-00227-a (VD) and US NIH GM083272 (PAJ). We thank David Slochower for help with Figs. 1.1 and 1.2.
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Dugina, V., Arnoldi, R., Janmey, P.A., Chaponnier, C. (2012). ACTIN. In: Kavallaris, M. (eds) Cytoskeleton and Human Disease. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-788-0_1
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