Abstract
Induced pluripotent stem cells (iPSCs) are pluripotent stem cells that are artificially derived from non-pluripotent cells by inducing “forced” expression of specific genes. Since first reported in 2006, iPSC technology has attracted great interest for its potential application in regenerative medicine and cell therapy. Cell types that require harvesting by medical intervention, such as skin fibroblasts and blood, have been the most common sources for deriving human iPSCs. The need to identify an alternative cell source that can be easily obtained from patients without an invasive procedure is well recognized. Here, we describe a simple method for generating human iPSCs from hair keratinocytes derived from the outer root sheath of plucked hair follicles, using a single polycistronic excisable lentiviral vector—the “STEMCCA” cassette.
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Novak, A. (2012). Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes. In: Amit, M., Itskovitz-Eldor, J. (eds) Atlas of Human Pluripotent Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-548-0_9
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DOI: https://doi.org/10.1007/978-1-61779-548-0_9
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