Abstract
While the culturing of human embryonic stem cells (hESCs) and induced pluripotent cells (iPSCs) with mouse embryonic fibroblasts (MEFs) or human foreskin fibroblasts may be adequate for research purposes, it is less suitable for clinical and industrial use due to the risk of exposure of the cells to animal pathogens and to variations between batches of MEFs and fetal bovine serum (FBS). To establish reproducible and defined cultures, devoid of animal products, feeder layers and animal products need to be replaced. This chapter describes layer-free culture systems that have been developed specifically for iPSCs.
Keywords
- Leukemia Inhibitory Factor
- Human Embryonic Stem Cell
- Feeder Layer
- Invitrogen Corporation
- Supporting Layer
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Amit, M., Itskovitz-Eldor, J. (2012). Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer-Free Conditions. In: Amit, M., Itskovitz-Eldor, J. (eds) Atlas of Human Pluripotent Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-548-0_3
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DOI: https://doi.org/10.1007/978-1-61779-548-0_3
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