Basic Considerations with Cell Sheets

  • Masayuki YamatoEmail author
  • Sebastian Sjöqvist
Part of the Stem Cell Biology and Regenerative Medicine book series (STEMCELL)


The foundation of cell sheet engineering is to harvest cells in a minimally destructive way. This way, the cells that retain their cell-to-cell contact show a higher expression of surface proteins, and retain their more highly developed functions than cells harvested enzymatically. In contrast to using biodegradable scaffolds for tissue engineering, cell sheets triggers a significantly lower immune response and do not cause an excess of extracellular matrix to be secreted around the transplanted cells. Cell sheets also tend to integrate more easily with host tissue. In this chapter, the basic concept and history of cell sheet engineering are discussed, including some of our own studies. This chapter focuses on using cell sheets for constructing corneas, minimizing postoperative complications after esophageal surgery, and transplantation of hormone-producing endocrine tissues.


Cell Sheet Support Membrane Biodegradable Scaffold Oral Mucosal Cell Oral Mucosal Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Adenosine triphosphate


Extracellular matrix


Ethylenediaminetetraacetic acid


Endoscopic mucosal resection


Endoscopic submucosal dissection


Free triiodothyronine


Free thyroxine


Lower critical solution temperature


Limbal stem cell deficiency


Poly(ethylene glycol)




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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Institute of Advanced Biomedical Engineering and ScienceTokyo Women’s Medical UniversityTokyoJapan

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