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Basic Considerations with Cell Sheets

  • Masayuki YamatoEmail author
  • Sebastian Sjöqvist
Chapter
Part of the Stem Cell Biology and Regenerative Medicine book series (STEMCELL)

Abstract

The foundation of cell sheet engineering is to harvest cells in a minimally destructive way. This way, the cells that retain their cell-to-cell contact show a higher expression of surface proteins, and retain their more highly developed functions than cells harvested enzymatically. In contrast to using biodegradable scaffolds for tissue engineering, cell sheets triggers a significantly lower immune response and do not cause an excess of extracellular matrix to be secreted around the transplanted cells. Cell sheets also tend to integrate more easily with host tissue. In this chapter, the basic concept and history of cell sheet engineering are discussed, including some of our own studies. This chapter focuses on using cell sheets for constructing corneas, minimizing postoperative complications after esophageal surgery, and transplantation of hormone-producing endocrine tissues.

Keywords

Cell Sheet Support Membrane Biodegradable Scaffold Oral Mucosal Cell Oral Mucosal Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ATP

Adenosine triphosphate

ECM

Extracellular matrix

EDTA

Ethylenediaminetetraacetic acid

EMR

Endoscopic mucosal resection

ESD

Endoscopic submucosal dissection

fT3

Free triiodothyronine

fT4

Free thyroxine

LCST

Lower critical solution temperature

LSCD

Limbal stem cell deficiency

PEG

Poly(ethylene glycol)

PIPAAm

Poly(N-isopropylacrylamide)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Institute of Advanced Biomedical Engineering and ScienceTokyo Women’s Medical UniversityTokyoJapan

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