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Directing Cell Fate Through Biomaterial Microenvironments

  • Kelly Clause
  • Jonathan Lam
  • Tatiana Segura
  • Thomas H. BarkerEmail author
Chapter
Part of the Stem Cell Biology and Regenerative Medicine book series (STEMCELL)

Abstract

Biomaterials offer discrete advantages over standard ECM systems, like matrigel, in the context of both fundamental stem cell biology and control of stem cell fate/phenotype. In particular, one can specifically design features into the 3D microenvironment with high levels of control. The fundamental limitation to date is that we currently lack the design rules for eliciting specific cellular and/or multicellular behaviors that may lead to true regenerative medicine. As the matrix biology and stem cell biology fields mature, biomaterial scientists continually look to these fields for inspiration and understanding of what features should be considered in the design of the “optimal” material for their specific application. In this chapter, we outline the basic emerging biological concepts leading toward a first set of design rules; those include physical or mechanical signals, chemical and/or biochemical signals, spatial orientation and positioning of signals, and finally, time-resolved display of signals. We highlight current biological findings that support these design rules (physical, chemical, and x, y, z, and t) and outline current efforts to develop biomaterial systems that enable both the decoupling or integration of these design criteria for tissue engineering and regenerative medicine applications.

Keywords

Stem Cell Differentiation Stem Cell Biology Integrin Cluster Growth Factor Delivery Regenerative Medicine Application 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ECM

Extracellular matrix

EDC

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

EGF

Epidermal growth factor

MSCs

Mesenchymal stem cells

PDGF

Platelet-derived growth factor

PEG

Polyethylene glycol

PLA

Polylactide

PLGA

Poly(lactic-co-glycolic acid)

RGD

Arginine-glycine-aspartic acid

Shh

Sonic hedgehog

sulfo-NHS

N-Hydroxysulfosuccinimide

VEGF

Vascular endothelial growth factor

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Kelly Clause
    • 1
  • Jonathan Lam
    • 2
  • Tatiana Segura
    • 3
  • Thomas H. Barker
    • 4
    Email author
  1. 1.The Wallace H. Coulter Department of Biomedical Engineering at Georgia TechEmory UniversityAtlantaUSA
  2. 2.Department of Biomedical EngineeringUniversity of CaliforniaLos AngelesUSA
  3. 3.Department of Chemical and Biomolecular EngineeringUniversity of CaliforniaLos AngelesUSA
  4. 4.The Wallace H. Coulter Department of Biomedical Engineering at Georgia TechEmory UniversityAtlantaUSA

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