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Nuclear Reprogramming and Stem Cells pp 59–69Cite as

Cell Fusion-Mediated Nuclear Reprogramming of Somatic Cells

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

Epigenetic reprogramming of nuclei from differentiated to pluripotent state can be induced by three experimental approaches: nuclear transfer, cell fusion, and transduction of transcription factors. In cell fusion between embryonic stem and somatic cells, stem cell-derived trans-acting factors function to confer pluripotency on somatic cell nuclei by overwriting the epigenotype of the undifferentiated state. Intensive analyses using inter-subspecific hybrid cells revealed that de-­condensation of nuclear chromatin is a key initial step for acquiring a fully reprogrammed nuclear status. The formation of de-condensed chromatin is a common molecular event seen in cell fusion-mediated reprogramming and the generation of induced pluripotent stem cells via the transduction of transcription factors. Thus, cell fusion is a powerful tool for providing information on the molecular mechanisms of nuclear reprogramming.

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Acknowledgment

Mr. K. Hirano is a research fellow of the Japanese Society for the Promotion of Science.

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Authors and Affiliations

  1. Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

    Kunio Hirano

  2. Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606–8507, Japan

    Takashi Tada

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  1. Kunio Hirano
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  2. Takashi Tada
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Correspondence to Takashi Tada .

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Editors and Affiliations

  1. Leeds Institute of Genetics,, Health & Therapeutics, University of Leeds, Leeds, LS2 9JT, United Kingdom

    Justin Ainscough

  2. Inst. Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Kyoto, 606-8507, Japan

    Shinya Yamanaka

  3. Inst. Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Kyoto, 606-8507, Japan

    Takashi Tada

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Hirano, K., Tada, T. (2011). Cell Fusion-Mediated Nuclear Reprogramming of Somatic Cells. In: Ainscough, J., Yamanaka, S., Tada, T. (eds) Nuclear Reprogramming and Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-225-0_6

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