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Clinical Application of Autologous Epithelial Stem Cells in Disorders of Squamous Epithelia

  • Nicolas Grasset
  • Yann Barrandon
Chapter
Part of the Stem Cell Biology and Regenerative Medicine book series (STEMCELL)

Abstract

Epidermis and epithelia lining the ocular surface, the oral cavity, the pharynx, the oesophagus, the larynx, and the vagina, called stratified squamous epithelia (SSE), contain stem/progenitor cells that support renewal and repair. Under appropriate conditions, these cells can be massively expanded in culture. Restoration of the integrity and the function of SSE is obtained by transplantation and engraftment of the autologous cultivated stem cells in case of several severe clinical conditions (e.g., extensive third-degree burns, limbal deficiency). Successful gene therapy for hereditary SSE disorders has also been achieved by means of correction of the genetic defect in cultivated autologous keratinocyte stem cells. However, the mechanisms controlling engraftment of the transplanted stem cells remain poorly understood, leading to unpredictable clinical results. Further fundamental investigations to explore the behaviour of the transplanted stem cells and their plasticity, anticipating the regulatory affairs main concerns, are needed for successful cell and gene therapy.

Keywords

Skin Cornea Engraftment Cell therapy Gene therapy 

Notes

Acknowledgments:

We are grateful to Ariane Rochat and François Gorostidi for helpful ­discussions. The work was supported by grants to Yann Barrandon from the EPFL, the CHUV and OptiStem, a consortium of the European Economic Community 7th Framework Program.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Nicolas Grasset
    • 1
    • 2
  • Yann Barrandon
    • 1
    • 2
  1. 1.Laboratory of Stem Cell Dynamics, School of Life SciencesEcole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland
  2. 2.Department of Experimental SurgeryCentre Hospitalier Universitaire Vaudois (CHUV)LausanneSwitzerland

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