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Stem Cells & Regenerative Medicine pp 141–153Cite as

Function of MicroRNA-145 in Human Embryonic Stem Cell Pluripotency

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

MicroRNAs are posttranscriptional regulators of gene expression, but their roles in controlling self-renewal and pluripotency of human embryonic stem cells (hESCs) remain unclear. Our recent study indicates that the level of microRNA-145 (miR-145) is low in hESCs but highly upregulated during differentiation. Endogenous miR-145 is sufficient to repress the 3′ untranslated regions of OCT4, SOX2, and KLF4. By downregulating OCT4 and SOX2, increased miR-145 inhibits hESC self-renewal, represses pluripotency, and induces lineage-restricted differentiation. Furthermore, the miR-145 promoter is bound and repressed by OCT4 in hESCs. Thus, miR-145 plays key roles in repressing hESC pluripotency and promoting differentiation. This work reveals a direct link between the core reprogramming factors and miR-145 and uncovers a double-negative feedback loop involving OCT4, SOX2, KLF4, and miR-145.

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Author information

Authors and Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, Neuroscience Research Institute, University of California at Santa Barbara, Santa Barbara, CA, 93106, USA

    Na Xu

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  1. Na Xu
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  2. Kenneth S. Kosik
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Correspondence to Na Xu .

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Editors and Affiliations

  1. Gene Expression Systems, Inc., Waltham, Massachusetts, USA

    Krishnarao Appasani

  2. Student Science, Wyllys Ave 45, Middletown, 06459, Connecticut, USA

    Raghu K. Appasani

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Xu, N., Kosik, K.S. (2011). Function of MicroRNA-145 in Human Embryonic Stem Cell Pluripotency. In: Appasani, K., Appasani, R. (eds) Stem Cells & Regenerative Medicine. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-860-7_9

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