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Production of Uniparental Embryonic Stem Cell Lines

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Trends in Stem Cell Biology and Technology

Abstract

Embryonic stem cells, or induced pluripotent cells derived from somatic cells, can yield differentiated progeny with potential applicability for tissue repair. This chapter describes the generation of embryonic stem cells from gamete-derived uniparental embryos. These embryonic stem cells can be patient-derived and potentially histocompatible with the gamete donor. The production of uniparental embryos followed by derivation of embryonic stem cells can be accomplished without producing fertilized zygotes, an advantage that avoids some ethical issues. We describe methods for the generation of uniparental embryonic stem cells from mouse uniparental embryos. We also address evaluation of the integrity of the lines generated, an essential criterion in interpreting differentiation assays in vivo and in vitro.

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  1. Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, 382 West Street Road, Kennett Square, PA, 19348, USA,

    Sigrid Eckardt & K. John McLaughlin

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  1. Sigrid Eckardt
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  2. K. John McLaughlin
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Correspondence to K. John McLaughlin .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Eckardt, S., McLaughlin, K.J. (2009). Production of Uniparental Embryonic Stem Cell Lines. In: Baharvand, H. (eds) Trends in Stem Cell Biology and Technology. Humana Press. https://doi.org/10.1007/978-1-60327-905-5_2

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