Abstract
All eukaryotic chromosome DNA ends have telomere DNA consisting of double-stranded telomere DNA repeats, which terminate in single-stranded 3′-overhangs called G-tails. Unprotected and exposed G-tails may be recognized by DNA damage signaling, inducing apoptotic signals that may cause dysfunction of tissues or initiation of carcinogenesis. Although G-tail length is essential for telomere end protection mechanism, the involvement of G-tail length in diseases is poorly understood. In this review, we introduce the importance of G-tail length and various protocols for G-tail measurement.
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Acknowledgments
This work was founded in part by a Grant-in-Aid for Scientific Research from the Ministory of Education, Culture, Sports, Science and Technology, Japan, and Japan Science and Technology Agency, Japan.
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Shimamoto, A., Aoki, E., Sera, A.M., Tahara, H. (2009). Protocol II: Importance and Methods of Telomere G-Tail Length Quantification. In: Hiyama, K. (eds) Telomeres and Telomerase in Cancer. Cancer Drug Discovery and Development. Humana Press. https://doi.org/10.1007/978-1-60327-879-9_15
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DOI: https://doi.org/10.1007/978-1-60327-879-9_15
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