Abstract
Sugar cane bagasse consists of hemicellulose (24%) and cellulose (38%), and bioconversion of both fractions to ethanol should be considered for a viable process. We have evaluated the hydrolysis of pretreated bagasse with combinations of cellulase, β-glucosidase, and hemicellulase. Ground bagasse was pretreated either by the AFEX process (2NH3: 1 biomass, 100 °C, 30 min) or with NH4OH (0.5 g NH4OH of a 28% [v/v] per gram dry biomass; 160 °C, 60 min), and composition analysis showed that the glucan and xylan fractions remained largely intact. The enzyme activities of four commercial xylanase preparations and supernatants of four laboratory-grown fungi were determined and evaluated for their ability to boost xylan hydrolysis when added to cellulase and β-glucosidase (10 filter paper units [FPU]: 20 cellobiase units [CBU]/g glucan). At 1% glucan loading, the commercial enzyme preparations (added at 10% or 50% levels of total protein in the enzyme preparations) boosted xylan and glucan hydrolysis in both pretreated bagasse samples. Xylanase addition at 10% protein level also improved hydrolysis of xylan and glucan fractions up to 10% glucan loading (28% solids loading). Significant xylanase activity in enzyme cocktails appears to be required for improving hydrolysis of both glucan and xylan fractions of ammonia pretreated sugar cane bagasse.
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References
Lynd, L. R., Wyman, C. E., & Gerngross, T. U. (1999). Biotechnology Progress, 15, 777–793.
Knauf, M., & Moniruzzaman, M. (2004). International Sugar Journal, 106, 147–150.
Rosgaard, L., Pedersen, S., Cherry, J. R., Harris, P., & Meyer, A. S. (2006). Biotechnology Progress, 22, 493–498.
Martin, C., Galbe, M., Wahlbom, C. F., Hahn-Hägerdal, B., & Jönsson, L. J. (2002). Enzyme and Microbial Technology, 31, 274–282.
Kaar, W. E., Gutierrez, C. V., & Kinoshita, C. M. (1998). Biomass and Bioenergy, 14, 277–287.
Fox, D. J., Gray, P. P., Dunn, N. W., & Marsden, W. L. (1987). Journal of Chemical Technology and Biotechnology, 40, 117–132.
Holtzapple, M. T., Jun, J.-H., Ashok, G., Patibandla, S. L., & Dale, B. E. (1991). Applied Biochemistry and Biotechnology, 28/29, 59–74.
Van Zyl, C., Prior, B. A., & du Preez, J. C. (1988). Applied Biochemistry and Biotechnology, 17, 357–369.
Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002). Microbiology and Molecular Biology Reviews, 66, 506–577.
Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y. Y., Holtzapple, M., et al. (2005). Bioresource Technology, 96, 673–686.
Adney, B., & Baker, J. (1996). NREL laboratory analytical procedure. In Laboratory Analytical Procedure No. 006. Golden, CO: National Renewable Energy Laboratory.
Wood, T. M., & Bhat, K. M. (1988). Methods in Enzymology, 160, 87–112.
Bailey, M. J., Biely, P., & Poutanen, K. (1992). Journal of Biotechnology, 23, 257–270.
Bradford, M. M. (1976). Analytical Biochemistry, 72, 248–254.
Dale, B. E., & Moriera, M. J. (1982). Biotechnology and Bioengineering Symposium, 12, 31–43.
Singh, S., Pillay, B., Dilsook, V., & Prior, B. A. (2000). Journal of Applied Microbiology, 88, 975–982.
Singh, S., Pillay, B., & Prior, B. A. (2000). Enzyme and Microbial Technology, 26, 502–508.
Berlin, A., Gilkes, N., Kilburn, D., Bura, R., Matkov, A., Skomatovsky, A., et al. (2005). Enzyme and Microbial Technology, 37, 175–184.
Dekker, R. F. H. (1986). Biotechnology and Bioengineering, 28, 1438–1442.
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Prior, B.A., Day, D.F. (2007). Hydrolysis of Ammonia-pretreated Sugar Cane Bagasse with Cellulase, β-Glucosidase, and Hemicellulase Preparations. In: Adney, W.S., McMillan, J.D., Mielenz, J., Klasson, K.T. (eds) Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-526-2_28
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DOI: https://doi.org/10.1007/978-1-60327-526-2_28
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