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Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources

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Applied Biochemistry and Biotecnology

Part of the book series: ABAB Symposium ((ABAB))

Abstract

Polyhydroxyalkanoates (PHAs) represent an environmentally effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85, 53, and 10% of the cell dry weight from methanol-enriched pulp and paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Using denaturing gradient gel electrophoresis of 16S-rDNA from polymerase chain reaction-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment. Most importantly, functional stability was maintained despite differing and contrasting microbial populations.

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Author information

Authors and Affiliations

  1. Department of Civil Engineering, University of Idaho, PO Box 441022, Moscow, Idaho, 83844-1022

    Erik R. Coats

  2. Department of Civil and Environmental Engineering, University of California Davis, 1 Shields Avenue, Davis, CA, 95616

    Frank J. Loge

  3. Biotechnology Department, Idaho National Laboratory, P. O. Box 1625, Idaho Falls, ID, 83415-2203

    William A. Smith & David N. Thompson

  4. Department of Civil and Environmental Engineering, Washington State University, Pullman, WA, 99164-2910

    Michael P. Wolcott

Authors
  1. Erik R. Coats
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  2. Frank J. Loge
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  3. William A. Smith
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  4. David N. Thompson
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  5. Michael P. Wolcott
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Corresponding author

Correspondence to Frank J. Loge .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Jonathan R. Mielenz

  2. Southern Regional Research Laboratory, USDA-ARS, USA

    K. Thomas Klasson

  3. National Renewable Energy Laboratory, USA

    William S. Adney  & James D. McMillan  & 

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© 2007 Humana Press Inc.

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Coats, E.R., Loge, F.J., Smith, W.A., Thompson, D.N., Wolcott, M.P. (2007). Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources. In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_74

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