Abstract
Production of biodiesel from waste cooking oils by a magnetic cell biocatalyst (MCB) immobilized in hydrophobic magnetic polymicrosphere is studied here. The cells of lipase-producing Bacillus subtilis were encapsulated within the net of hydrophobic carrier with magnetic particles (Fe3O4), and the secreted lipase can be conjugated with carboxyl at the magnetic polymicrosphere surface. Environmental scanning electron microscope, transmission electron microscope, and vibrating magnetometer, and so on were used to characterize the MCB. The MCB was proved to be superparamagnetic; and could be recovered by magnetic separation; moreover it could be regenerated under 48 h of cultivation. When methanolysis is carried out using MCB with waste cooking oils under stepwise additions of methanol, the methyl esters in the reaction mixture reaches about 90% after 72h reaction in a solvent-free system. The process presented here is environmentally friendly and simple without purification and immobilized process required by the current lipase-catalyzed process. Therefore, the process is very promising for development of biodiesel fuel industry.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Du, W., Xu, Y. Y., Liu, D. H., and Zeng, J. (2004), J. Mol. Catalysis B: Enzymatic 30, 125–129.
Köse, Ö., Tüter, M., and Aksoy, A. H. (2002), Bioresour. Technol. 83, 125–129.
Iso, M., Chen, B. X., Eguchi, M., Kudo, T., and Shrestha, S. (2001), J. Mol. Catalysis B: Enzymatic 16, 53–58.
Oda, M., Kaieda, M., Hama, S., et al (2005), Biochem. Eng. J.. 23, 45–51.
Bahar, T. and Celebi, S. (2000). Enzyme Microb. Technol. 26, 28–37.
Kondo, A., Liu, Y., Furuta, M., Fujita, Y., Matsumoto, T., and Fukuda, H., (2000), Enzyme Microbial. Technol. 27, 806–811.
Ban, K., Hama, S., Nishizuka, K., et al. (2002), J. Mol. Catalysis B: Enzymatic 17, 157–165.
Ban, K., Kaieda, M., Matsumotoc, T., Kondo, A., and Fukuda, H. (2001), Biochem. Eng. J. 8, 39–43.
Ruiz, C., Blanco, A., Pastor, F. I. J., and. Diaz, P. (2002), FEMS Microbiol. Lett. 217, 263–267.
Eggert, T., Brockmeier, U., Dröge, M. J., Quax, W. J., and Jaeger, K. E. (2003), FEMS Microbiol. Lett. 225, 319–324.
Ruiz, C., Pastor, F. I. J., and Diaz, P. (2003), Lett. Appl. Microbiol. 37, 354–359.
Eggert, T., Pouderoyen, G. V., Pencreac’h, G., et al. (2002)., Colloids Surf. B: Biointerfaces. 26, 37–46.
Pouderoyen, G. V., Eggert, T., Jaeger, K. E., and Dijkstral, B. W. (2001), J. Mol. Biol. 309, 215–226.
Eggert, T., Pouderoyen, G. V., Dijkstrab, B. W. and Jaeger, K. E. (2001), FEBS Lett. 502, 89–92.
M. Z. Y. Guan, Y. P., Liu, X. Q., and Liu, H. Z. (2005), Chin. J. Chem. Eng. 13, 239–243.
Bean, C. P. and Livingston, J. D. (1959), J. Appl. Phys. 30, 120–129.
Xue, B. and Sun, Y. (2002), J. Chromatogr. A. 947, 185–193.
Chantrell, R. W. and Popplewell, J. (1978), IEEE Trans. Mag. 14, 975–980.
Zhang, M. L. and Sun, Y. (2001), J. Chromatogr. A 912, 31–38.
Kobayashi, H. and Matsunaga, T. (1991), J. Colloid Interface Sci. 141, 505–511.
Kröbitz, W. (1999), Renewasble Energy 16, 1078–1083.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this chapter
Cite this chapter
Ying, M., Chen, G. (2007). Study on the Production of Biodiesel by Magnetic Cell Biocatalyst Based on Lipase-Producing Bacillus subtilis . In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_65
Download citation
DOI: https://doi.org/10.1007/978-1-60327-181-3_65
Publisher Name: Humana Press
Print ISBN: 978-1-60327-180-6
Online ISBN: 978-1-60327-181-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)