Proteomic Analysis in Cancer Patients

  • Yasuhiro Kuramitsu
  • Kazuyuki Nakamura
Part of the Cancer Drug Discovery and Development™ book series (CDD&D)

Summary

The term proteome means the total protein complement of a genome, and proteomics means the analysis for proteome. The combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) is a proteomic method of high-throughput analysis of protein expression. By using this 2-DE and MS, proteomic studies have identified many proteins that may be involved in the pathogenic mechanism of cancers. These studies analyzed cancer cell lines, as well as cancer tissues or serum from patients.

In the present study, we analyzed proteome in hepatocellular carcinoma (HCC), esophageal cancer, and pancreatic cancer tissues. We identified many proteins whose expression in cancer tissues was different from corresponding non-cancerous tissues by using 2-DE and MS. Furthermore, we identified some auto-antibodies reacting to proteins in HCC cancer tissues. In this chapter, we will describe the method, our experimental result, and reports from other researchers about proteomic analysis in cancer patients.

Key Words

Proteomics hepatocellular carcinoma HCC esophageal cancer pancreatic cancer auto-antibody 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yasuhiro Kuramitsu
    • 1
  • Kazuyuki Nakamura
    • 1
  1. 1.Department of Biochemistry and Functional ProteomicsYamaguchi University Graduate School of MedicineYamaguchiJapan

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