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Functional Molecular Imaging of Prostate Cancer Lymph Node Metastases: Adenovirus-Mediated Lymph Node Detection

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Book cover From Local Invasion to Metastatic Cancer

Part of the book series: Current Clinical Oncology ((CCO))

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Abstract

Like other solid epithelial tumors, nodal involvement in prostate cancer signifies a poor clinical outcome. Yet, the lack of standard pelvic lymph node (LN)-sampling procedures and specific noninvasive imaging approaches to accurately assess nodal involvement is hampering the management decision. This chapter will discuss the debates in the clinical community on LN dissection and the development of various imaging modalities for prostate cancer. We will also describe a novel approach of using recombinant human adenoviral vectors (Ads) to directly map nodal metastases in experimental models of human prostate cancer. By exploiting the innate lymphotrophic properties of adenovirus and the prostate-restricted expression of imaging reporter genes incorporated into the viral vector, we were able to produce bioluminescent or positron emission tomography (PET) signals that correlate with the presence of metastatic lesions in the draining LNs. Significantly, this approach enables the direct PET visualization of sentinel lymph node (SLN) metastases, without the need for lymphadenectomy. We believe that developing and implementing more effective diagnostic imaging modalities to assess nodal status could improve the outcomes for patients with advanced stage prostate cancer.

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Acknowledgement

We thank Ms. Sok Boon Koh for performing the electron microscopy of adenovirus and providing the images. This work is supported by NCI SPORE program P50 CA092131, RO1 CA101904, and Prostate Cancer Foundation. J.B. is supported by Department of Defense, CDMRP 07-1-0064 (prostate cancer predoctoral training grant). S.P. is supported by the career developmental program of UCLA ICMIC (P50 CA86306 to H.R. Herschman) grant.

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Authors and Affiliations

  1. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA

    Saul J. Priceman MS & Jeremy B. Burton PhD

  2. Department of Molecular and Medical Pharmacology and Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA

    Lily Wu MD, PhD

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  1. Saul J. Priceman MS
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  2. Jeremy B. Burton PhD
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Editors and Affiliations

  1. Division of Surgical Oncology, University of California, San Francisco, Divisadero St. 1600, San Francisco, 94115, USA

    Stanley P. L. Leong

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Priceman, S.J., Burton, J.B., Wu, L. (2009). Functional Molecular Imaging of Prostate Cancer Lymph Node Metastases: Adenovirus-Mediated Lymph Node Detection. In: Leong, S. (eds) From Local Invasion to Metastatic Cancer. Current Clinical Oncology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-087-8_16

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  • DOI: https://doi.org/10.1007/978-1-60327-087-8_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-086-1

  • Online ISBN: 978-1-60327-087-8

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