The Statistical Design and Interpretation of Microarray Experiments

  • Kevin K. Dobbin
  • Richard M. Simon
Chapter
Part of the Cancer Drug Discovery and Development book series (CDD&D)

Abstract

This chapter reviews major issues related to design and interpretation of microarray experiments. Important aspects of design covered include identification of experimental objectives, treatment of batch effects, selection of replication and pooling levels, determining sample size for class comparison and class prediction, and optimal allocation of samples to arrays and labels in dual label experiments. Aspects of interpretation focus on class prediction issues, including case selection, external vs. internal validation, and pitfalls in cross-validation.

Keywords

Microarrays Experimental design Sample size Prediction Validation  

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Kevin K. Dobbin
    • 1
  • Richard M. Simon
    • 1
  1. 1.Biometric Research BranchNational Cancer InstituteBethesdaUSA

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