Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Corbel MJ, WJB-M (1984) Genus Brucella. In: Bergey's Manual of Systematic Bacteriology Krieg NR, ed), pp. 377–388. Williams and Wilkins, Baltimore, MD.
Corbel MJ (1985) DNA analysis of Brucella : Present and future. Brucella melitensis Plommet JMVM, ed),pp. 21–27. Martinus Nijhoff, Dordecht, The Netherlands.
Verger JM, Grimont F, Grimont PA, Grayon M (1987) Taxonomy of the genus Brucella. Ann Inst Pasteur Microbiol 138:235–238.
Gandara B, Merino AL, Rogel MA, Martinez-Romero E (2001) Limited genetic diversity of Brucella spp. J Clin Microbiol 39:235–240.
Yagupsky P, Baron EJ (2005) Laboratory exposures to bru-cellae and implications for bioterrorism. Emerg Infect Dis 11:1180–1185.
Jensen AE, Cheville NF, Thoen CO, MacMillan AP, Miller WG (1999) Genomic fingerprinting and development of a dendrogram for Brucella spp. isolated from seals, porpoises, and dolphins. J Vet Diagn Invest 11:152–157.
Allardet-Servent A, Bourg G, Ramuz M, Pages M, Bellis M, Roizes G (1988) DNA polymorphism in strains of the genus Brucella. J Bacteriol 170:4603–4607.
Bricker BJ, Halling SM (1994) Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Bru-cella suis bv. 1 by PCR. J Clin Microbiol 32:2660–2666.
Dees S, Thanabalasundrum S, Moss CW, Hollis DG, Weaver RE (1980) Cellular fatty acid composition of group IVe, a non-saccharolytic organism from clinical sources. J Clin Microbiol 11:664–668.
Ouahrani S, Michaux S, Sri Widada J, Bourg G, Tournebize R, Ramuz M, Liautard JP (1993) Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies. J Gen Microbiol 139:3265–3273.
Roop II RM Price ML, Dunn BE, Boyle SM, Sriranganathan N, Schurig GG (1992) Molecular cloning and nucleotide sequence analysis of the gene encoding the immunoreactive Brucella abortus Hsp60 protein, BA60K. Microb Pathog 12: 47 – 62.
Vizcaino N, Cloeckaert A, Verger J, Grayon M, Fernandez-Lago L (2000) DNA polymorphism in the genus Brucella. Microbes Infect 2:1089–1100.
Hoyer BH, McCullough NB (1968) Polynucleotide homologies of Brucella deoxyribonucleic acids. J Bacteriol 95: 444–448.
Tcherneva E, Rijpens N, Jersek B, Herman LM (2000) Differentiation of Brucella species by random amplified polymorphic DNA analysis. J Appl Microbiol 88:69–80.
Michaux-Charachon S, Bourg G, Jumas-Bilak E, Guigue-Talet P, Allardet-Servent A, O'Callaghan D, Ramuz M (1997) Genome structure and phylogeny in the genus Brucella. J Bacteriol 179:3244–3249.
Bricker BJ, Ewalt DR, Halling SM (2003) Brucella ‘HOOF-Prints’: strain typing by multi-locus analysis of variable number tandem repeats (VNTRs). BMC Microbiol 3:15.
Whatmore AM, Shankster S, Perrett LL, Murphy TJ, Brew SD, Thirlwall RE, Cutler SJ, MacMillan A. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J Clin Microbiol 44:1982–1993.
Keim P, Van Ert MN, Pearson T, Vogler AJ, Huynh LY, W a g -ner DM (2004) Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. Infect Genet Evol 4:205–213.
Klevytska AM, Price LB, Schupp JM, Worsham PL, Wong J, Keim P (2001) Identification and characterization of variable- number tandem repeats in the Yersinia pestis genome. J Clin Microbiol 39:3179–3185.
Johansson A, Farlow J, Larsson P, Dukerich M, Chambers E, Bystrom M, Fox J, Chu M, Forsman M, Sjostedt A, Keim P (2004) Worldwide genetic relationships among Francisella tularensis isolates determined by multiple-locus variable-number tandem repeat analysis. J Bacteriol 186:5808–5818.
Girard JM, Wagner DM, Vogler AJ, Keys C, Allender CJ, Drickamer LC, Keim P (2004) Differential plague-transmission dynamics determine Yersinia pestis population genetic structure on local, regional, and global scales. Proc Natl Acad Sci USA 101:8408–8413.
Keim P, Klevytska AM, Price LB, Schupp JM, Zinser G, Smith KL, Hugh-Jones ME, Okinaka R, Hill KK, Jackson PJ (1999) Molecular diversity in Bacillus anthracis. J Appl Microbiol 87:215–217.
Keim P, Price LB, Klevytska AM, Smith KL, Schupp JM, Oki-naka R, Jackson PJ, Hugh-Jones ME (2000) Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J Bacteriol 182:2928–2936.
Hoffmaster AR, Fitzgerald CC, Ribot E, Mayer LW, Popovic T (2002) Molecular subtyping of Bacillus anthracis and the 2001 bioterrorism-associated anthrax outbreak, United States. Emerg Infect Dis 8:1111–1116.
Lowell JL, Wagner DM, Atshabar B, Antolin MF, Vogler AJ, Keim P, Chu MC, Gage KL (2005) Identifying sources of human exposure to plague. J Clin Microbiol 43:650–656.
Farlow J, Wagner DM, Dukerich M, Stanley M, Chu M, Kubota K, Petersen J, Keim P (2005) Francisella tularensis in the United States. Emerg Infect Dis 11:1835–1841.
Gur-Arie R, Cohen CJ, Eitan Y, Shelef L, Hallerman EM, Kashi Y (2000) Simple sequence repeats in Escherichia coli: abundance, distribution, composition, and polymorphism. Genome Res 10:62–71.
Boyer JC, Yamada NA, Roques CN, Hatch SB, Riess K, Farber RA (2002) Sequence dependent instability of mononucleotide microsatellites in cultured mismatch repair proficient and deficient mammalian cells. Hum Mol Genet 11:707–713.
Vogler AJ, Keys C, Nemoto Y, Colman RE, Jay Z, Keim P (2006) Effect of repeat copy number on variable-number tandem repeat mutations in Escherichia coli O157:H7. J Bacteriol 188:4253–4263.
DeWoody JA, Schupp J, Kenefic L, Busch J, Murfitt L, Keim P (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. Biotechniques 37:348, –350, 352.
Weir BS (1990) Genetic Data Analysis, Sinauer Associates, Inc., Sunderland, MA.
Ficht TA, Husseinen HS, Derr J, Bearden SW (1996) Species-specific sequences at the omp2 locus of Brucella type strains. Int J Syst Bacteriol 46:329–331.
Ouahrani-Bettache S, Soubrier MP, Liautard JP (1996) IS6501-anchored PCR for the detection and identification of Brucella species and strains. J Appl Bacteriol 81:154–160.
DelVecchio VG, Kapatral V, Elzer P, Patra G, Mujer CV (2002) The genome of Brucella melitensis. Vet Microbiol 90:587–592.
Acknowledgements
This work was supported by the Department of Energy, Chemical and Biological Non-Proliferation program (Analysis for Bacterial Strain Identification Sponsor Award DE-FG03-00NN2010), the Department of Homeland Security—HSARPA, and the Northern Arizona University Raymond and Ruth Cowden Endowment for Microbiology. We thank Gery Allan (Northern Arizona University) for discussion on various methods for phylogenetic analysis.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Humana Press, a part of Springer Science+Business Media, LLC
About this paper
Cite this paper
Huynh, L.Y. et al. (2008). Multiple Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) of Brucella spp. Identifies Species-Specific Markers and Insights into Phylogenetic Relationships. In: Georgiev, V.S., Western, K.A., McGowan, J.J. (eds) National Institute of Allergy and Infectious Diseases, NIH. Infectious Disease. Humana Press. https://doi.org/10.1007/978-1-59745-569-5_6
Download citation
DOI: https://doi.org/10.1007/978-1-59745-569-5_6
Publisher Name: Humana Press
Print ISBN: 978-1-934115-77-0
Online ISBN: 978-1-59745-569-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)