Abstract
The transport of catalysts (chemicals and enzymes) within plant biomass is believed to be a major bottleneck during thermochemical pretreatment and enzymatic conversion of lignocellulose. Subjecting biomass to size reduction and mechanical homogenization can reduce catalyst transport limitations; however, such processing adds complexity and cost to the overall process. Using high-resolution light microscopy, we have monitored the transport of an aqueous solution of Direct Blue-I (DB-I) dye through intact corn internodes under a variety of impregnation conditions. DB-I is a hydrophilic anionic dye with affinity for cellulose. This model system has enabled us to visualize likely barriers and mechanisms of catalyst transport in corn stems. Microscopic images were compared with calculated degrees of saturation (i.e., volume fraction of internode void space occupied by dye solution) to correlate impregnation strategies with dye distribution and transport mechanisms. Results show the waxy rind exterior and air trapped within individual cells to be the major barriers to dye transport, whereas the vascular bundles, apoplastic continuum (i.e., the intercellular void space at cell junctions), and fissures formed during the drying process provided the most utilized pathways for transport. Although representing only 20–30% of the internode volume, complete saturation of the apoplast and vascular bundles by fluid allowed dye contact with a majority of the cells in the internode interior.
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References
Thomas, S., Porter, S., Jurich, J., et al. (2004), Technical Report FY04-561, National Renewable Energy Laboratory, Golden, CO.
McMillan, J. D. (1994), ACS Symposium Series 566, 292–324.
Kazi, K. M. F., Jollez, P., and Chornet, E. (1998), Biomass Bioenergy 15, 125–141.
Ramos, L. P. (2003), Quimica Nova 26, 863–871.
Malkov, S., Tikka, P., and Gullichsen, J. (2001), Paper Timber 83, 468–473.
Esteghlalian, A., Hashimoto, A. G., Fenske, J. J., and Penner, M. H. (1997), Biores. Technol. 59, 129–136.
Soderstrom, J., Pilcher, L., Galbe, M., and Zacchi, G. (2003), Biomass and Bioenergy 24, 475–486.
Zhu, Y., Lee, Y. Y., and Elander, R. T. (2004), Appl. Biochem. Biotechnol. 117, 103–114
Schell, D. J., Farmer, J., Newman, M., and McMillan, J. D. (2003), Appl. Biochem Biotechnol. 105/108, 69–85.
Aden, A., Ruth, M., Ibsen, K., et al. (2002), Technical Report NREL/TP-510-32438, National Renewable Energy Laboratory, Golden, CO.
Kim, K. H., Tucker, M. P., and Nguyen, Q. A. (2002), Biotechnol. Prog. 18, 489–494.
Decker, S. and Vinzant, T. (2004), Office of Biomass Program E Milestone, ID No. FY04-602.
Raven, P. H., Evert, R. F., and Eichhorn, S. E. (1992), Biology of Plants, 5th edition, Worth Publishers, Inc., New York.
Horobin, R. W. and Kiernan, J. A. (2002), Conn’s Biological Stains, 10th edition, Biological Stain Commission, Oxford.
Mani, S., Tabil, L. G., and Sokhansanj, S. (2004), Canadian Biosys. Eng. 46, 55–61.
Kim, S. B. and Lee, Y. Y. (2002), Biores. Technol. 83, 165–171.
Tucker, M. P., Kim, K. H., Newman, M. M., and Nguyen, Q. A. (2003), Appl. Biochem. Biotechnol. 105, 165–177.
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Viamajala, S. et al. (2006). Catalyst Transport in Corn Stover Internodes. In: McMillan, J.D., Adney, W.S., Mielenz, J.R., Klasson, K.T. (eds) Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-59745-268-7_42
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DOI: https://doi.org/10.1007/978-1-59745-268-7_42
Publisher Name: Humana Press
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