Abstract
Several factors inherent in autopsy pathology conspire to prevent or diminish good tissue preservation. There is the delay in starting the dissection, which may be hoursto several days in duration. In many instances, the body will remain unrefridgerated on the clinical floor during part of that delay. Autolysis is further hastened in obese patients because of decreased dissipation of body heat. Second, the autopsy procedure takes several hours and is often conducted under bright lights, providing a setting for continued autolysis. Third, the large amount of blood accompanying the organs can significantly reduce the efficacy of fixatives and finally, adequate fixation, using a fixative-to-tissue ratio as low as 5–10 to 1 still requires large containers and prohibitively large quantities of fixative.
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References
Prophet EB, Mills B, Arrington JB, Sobin LH, eds. Laboratory Methods in Histotechnology. Armed Forces Institute of Pathology. American Registry of Pathology, Washington, DC, 1992.
Rosen Y, Ahuja SC. Ice crystal distortion of formalin-fixed tissues following freezing. Am J Surg Pathol 1977;1:179–181.
Herzog V, Fahimi HD. The effect of glutaraldehyde on catalase. J Cell Biol 1974;60:303–311.
Leong AS, Daymon ME, Milios J. Microwave irradiation as a form of fixation for light and electron microscopy. J Pathol 1985;146: 313–321.
Leong AS-Y, Milios J, Duncis CG. Antigen preservation in microwave-irradiated tissues: a comparison with formaldehyde fixation. J Pathol 1988;156:275–282.
Leong AS-Y, Duncis CG. A method of rapid fixation of large biopsy specimens using microwave irradiation. Pathology 1986;18:222–225.
Leong AS-Y. Microwave fixation and rapid processing in a large throughput histopathology laboratory. Pathology 1991;23:271–273.
Baker PB. Electron microscopy. In: Hutchins GM, ed. Autopsy, Performance and Reporting. College of American Pathologists, Northfield, UL, 1990, pp. 138–140.
McDowell EM, Tromp BF. Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol Lab Moo 1976;100:405–414.
Robards AW, Wilson AJ (principal eds.). Procedures in Electron Microscopy. Centre for Cell & Tissue Research, The University, York, UK, John Wiley & Sons, New York, 1993.
Baker PB. Special autopsy studies. In: Hutchins GM, ed. Autopsy, Performance and Reporting. College of American Pathologists, Northfield, IL, 1990, pp. 142–146.
Landas S, Turner JW, Moore KC, Mitros FA. Demonstration of iron and thorium in autopsy tissues by X-ray microanalysis. Arch Pathol Lab Med 1984;108:231–233.
Sigee DC, Morgan AJ, Sumner AT, Warley A, eds. X-ray Microanalysis in Biology: Experimental Techniques and Applications. Cambridge University Press, Cambridge, UK, 1993.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Waters, B.L. (2009). Fixation and Transport of Autopsy Material. In: Waters, B.L. (eds) Handbook of Autopsy Practice. Humana Press. https://doi.org/10.1007/978-1-59745-127-7_15
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DOI: https://doi.org/10.1007/978-1-59745-127-7_15
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